Ambion™ mMESSAGE mMACHINE™ High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 μg of 7-methyl guanosine capped RNA from 1 μg of template DNA. Each kit includes sufficient reagents for 25 reactions. • High yields of capped RNA—up to 39 μg of RNA per reaction • Extra convenience—cap analog-nucleotide mix provided • Simplified reaction format • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 μl reaction during a 2 hour incubation, mMESSAGE mMACHINE™ High Yield Capped RNA Transcription will yield up to 35 μg of capped RNA. This is 10–50 times the yield obtained with conventional in vitro transcription reactions. The ratio of cap analog to GTP has been optimized to allow the best compromise between yield (15–35 μg) and proportion of transcripts that are capped (80%). The mMESSAGE mMACHINE™ kits also contain a LiCl precipitation solution that is efficient for separating proteins and unincorporated nucleotides (including free cap analog) from the capped RNA, allowing an increased efficiency of translation. mMESSAGE mMACHINE™ Kits are only optimized for use with the polymerases included in the kit. Using a different polymerase than what is supplied in the kit may result in low yields. Each mMESSAGE mMACHINE™ Kit contains 57 U of cap analog, an outstanding value for the cost.
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Tipo de producto final
ARNm sintético, ARNm
Formato
Kit
Tipo de muestra
ARNm, ARNm sintético
Para utilizar con (aplicación)
Transcripción in vitro, ARNm sintético, ARNm, transfección, LNP, GENEArt, plasmídico de ADN linealizado, suministro, productos de PCR
N.º de reacciones
25 reacciones
Línea de productos
Ambion™, mMESSAGE mMACHINE™
Tipo de producto
Kit de transcripción in vitro T3
Cantidad
25 reacciones
Promotor
T3
Condiciones de envío
Hielo seco
Producción
20-30 µg
Material de partida
Productos de PCR, plásmido de ADN linealizado
Contenido y almacenamiento
Mezcla de enzimas T3, tampón de reacción 10X 2XNTP⁄CAP, solución ATP, solución CAP, solución GTP, pTRI-Xef, ADNasa TURBO, solución de parada de acetato de amonio, solución de cloruro de litio y tampón de carga de gel II. Todos se almacenan a –20 °C. El agua sin nucleasas puede almacenarse a cualquier temperatura.
Figuras
Documentos y descargas
Certificados
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