In vitro RNA transcription reactions are generally used for two distinct purposes: the synthesis of labeled probes, and the synthesis of large amounts of unlabeled RNA. Additionally, capped RNA synthesized in transcription reactions is used for microinjection, in vitro translation, and transfection. Our RNA experts have developed nucleotides, polymerases, modifying enzymes, and kits for high-yield capped RNA transcription, large-scale transcription, and transcription of short DNA templates. We also offer a complete line of Ambion products for in vitro transcription and translation studies.

Tools for In Vitro Transcription & Translation

In Vitro Transcription

The ability to synthesize RNA in the laboratory is critical to many techniques. Radiolabeled and nonisotopically labeled RNA probes, generated in small-scale transcription reactions, can be used in blot hybridizations and nuclease protection assays. mRNA amplification for gene array analysis requires the use of large-scale transcription reactions.

In Vitro Translation

In vitro synthesis of proteins in cell-free extracts is an important tool for molecular biologists and has a variety of applications, including the rapid identification of gene products (e.g., proteomics), localization of mutations through synthesis of truncated gene products, protein folding studies, and incorporation of modified or unnatural amino acids for functional studies.