SYTOX™ Blue Nucleic Acid Stain - 5 mM Solution in DMSO
SYTOX™ Blue Nucleic Acid Stain - 5 mM Solution in DMSO
Citations & References (27)
Invitrogen™

SYTOX™ Blue Nucleic Acid Stain - 5 mM Solution in DMSO

SYTOX® Blue nucleic acid stain is an excellent blue-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, makingRead more
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Catalog NumberQuantity
S11348250 μL
Catalog number S11348
Price (EUR)
542,00
Each
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Quantity:
250 μL
Price (EUR)
542,00
Each
Add to cart
SYTOX® Blue nucleic acid stain is an excellent blue-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population. The excitation/emission maxima for the dye bound to DNA are 444/480 nm.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorBlue
Detection MethodFluorescence
Dye TypeCell-Permeant
Emission480 nm
Excitation Wavelength Range444 nm
For Use With (Equipment)Fluorescence Microscope
FormSolution
FormatTube(s)
Product LineSYTOX
Quantity250 μL
Shipping ConditionRoom Temperature
Volume (Metric)250 μL
Label TypeFluorescent Dye
Product TypeNucleic Acid Stain
SubCellular LocalizationNucleic Acids
Unit SizeEach
Contents & Storage
Store in freezer at -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am using SYTOX AAdvanced as a dead cell stain, but all of my cells are labeling even though I am certain that they are supposed to be alive. These are adherent cells that I have trypsinized. Why am I getting false-dead signals?

SYTOX AAdvanced labels only dead cells because it is a cell impermeant dye. The dye can only enter cells that have a compromised plasma membrane. Trypsinization may cause temporary disruption of the plasma membrane, sufficient to allow staining with a cell impermeant dye. You can reduce the “false-dead” problem by either reducing the amount of trypsin and/or reduce the incubation time for trypsinization or use a gentler dissociation reagent such as TrypLE Express, TrypLESelect reagents, or Versene. After trypsinization, wash well, and if possible, allow a recovery time in normal culture media before staining with any of the SYTOX dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long can I leave SYTOX Blue Nucleic Acid Stain - 5 mM Solution in DMSO on my cells during imaging?

SYTOX dyes are only tested for end-point assays, not for leaving in cell solutions long term. Therefore, we do not recommend using SYTOX Blue Nucleic Acid Stain - 5 mM Solution in DMSO for long-term imaging.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (27)

Citations & References
Abstract
Signaling at the gliovascular interface.
Authors:Simard M, Arcuino G, Takano T, Liu QS, Nedergaard M
Journal:J Neurosci
PubMed ID:14534260
'Advances in fluorescent calcium indicating dyes over the past decade have identified calcium signaling as the tool by which astrocytes communicate among themselves and with neighboring neurons. Studies of astrocyte-neuron interactions have shown that calcium signaling is a potent modulator of the strength of both excitatory and inhibitory synapses. The ... More
Phosphatidylserine-dependent engulfment by macrophages of nuclei from erythroid precursor cells.
Authors:Yoshida H, Kawane K, Koike M, Mori Y, Uchiyama Y, Nagata S
Journal:Nature
PubMed ID:16193055
'Definitive erythropoiesis usually occurs in the bone marrow or fetal liver, where erythroblasts are associated with a central macrophage in anatomical units called ''blood islands''. Late in erythropoiesis, nuclei are expelled from the erythroid precursor cells and engulfed by the macrophages in the blood island. Here we show that the ... More
Trogocytosis by Entamoeba histolytica contributes to cell killing and tissue invasion.
Authors:Ralston KS, Solga MD, Mackey-Lawrence NM, Somlata, Bhattacharya A, Petri WA,
Journal:
PubMed ID:24717428
'Entamoeba histolytica is the causative agent of amoebiasis, a potentially fatal diarrhoeal disease in the developing world. The parasite was named '
Delivery of macromolecules into live cells by simple co-incubation with a peptide.
Authors:Lee YJ, Erazo-Oliveras A, Pellois JP,
Journal:Chembiochem
PubMed ID:20029930
'n this report, we test the hypothesis that optimized cell-penetrating peptides (CPPs) might deliver macromolecules to the cytosol of live cells by simple co-incubation and without the requirement for any type of conjugation, whether covalent or noncovalent. This hypothesis is based on the observation that the binding of TAT and ... More
Assessment of fluorochromes for two-photon laser scanning microscopy of biofilms.
Authors:Neu TR, Kuhlicke U, Lawrence JR
Journal:Appl Environ Microbiol
PubMed ID:11823234
A major limitation for the use of two-proton laser scanning microscopy (2P-LSM) in biofilm and other studies is the lack of a thorough understanding of the excitation-emission responses of potential fluorochromes. In order to use 2P-LSM, the utility of various fluorochromes and probes specific for a range of biofilm constituents ... More
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