AuthorsSimard M, Arcuino G, Takano T, Liu QS, Nedergaard M
JournalJ Neurosci
PubMed ID14534260
'Advances in fluorescent calcium indicating dyes over the past decade have identified calcium signaling as the tool by which astrocytes communicate among themselves and with neighboring neurons. Studies of astrocyte-neuron interactions have shown that calcium signaling is a potent modulator of the strength of both excitatory and inhibitory synapses. The ... More
Phosphatidylserine-dependent engulfment by macrophages of nuclei from erythroid precursor cells.
AuthorsYoshida H, Kawane K, Koike M, Mori Y, Uchiyama Y, Nagata S
JournalNature
PubMed ID16193055
'Definitive erythropoiesis usually occurs in the bone marrow or fetal liver, where erythroblasts are associated with a central macrophage in anatomical units called ''blood islands''. Late in erythropoiesis, nuclei are expelled from the erythroid precursor cells and engulfed by the macrophages in the blood island. Here we show that the ... More
Trogocytosis by Entamoeba histolytica contributes to cell killing and tissue invasion.
AuthorsRalston KS, Solga MD, Mackey-Lawrence NM, Somlata, Bhattacharya A, Petri WA,
Journal
PubMed ID24717428
'Entamoeba histolytica is the causative agent of amoebiasis, a potentially fatal diarrhoeal disease in the developing world. The parasite was named '
Delivery of macromolecules into live cells by simple co-incubation with a peptide.
AuthorsLee YJ, Erazo-Oliveras A, Pellois JP,
JournalChembiochem
PubMed ID20029930
'n this report, we test the hypothesis that optimized cell-penetrating peptides (CPPs) might deliver macromolecules to the cytosol of live cells by simple co-incubation and without the requirement for any type of conjugation, whether covalent or noncovalent. This hypothesis is based on the observation that the binding of TAT and ... More
Assessment of fluorochromes for two-photon laser scanning microscopy of biofilms.
AuthorsNeu TR, Kuhlicke U, Lawrence JR
JournalAppl Environ Microbiol
PubMed ID11823234
A major limitation for the use of two-proton laser scanning microscopy (2P-LSM) in biofilm and other studies is the lack of a thorough understanding of the excitation-emission responses of potential fluorochromes. In order to use 2P-LSM, the utility of various fluorochromes and probes specific for a range of biofilm constituents ... More
A simple way to identify non-viable cells within living plant tissue using confocal microscopy.
AuthorsTruernit E, Haseloff J,
JournalPlant Methods
PubMed ID18573203
ABSTRACT: BACKGROUND: Plant cell death is a normal process during plant development. Mutant plants may exhibit misregulation of this process, which can lead to severe growth defects. Simple ways of visualising cell death in living plant tissues can aid the study of plant development and physiology. RESULTS: Spectral variants of ... More
Essential role of histidine 20 in the catalytic mechanism of Escherichia coli peptidyl-tRNA hydrolase.
AuthorsGoodall JJ, Chen GJ, Page MG
JournalBiochemistry
PubMed ID15078105
The peptidyl-tRNA hydrolase (Pth) enzyme plays an essential role in recycling tRNA from peptidyl-tRNA that has prematurely dissociated from the ribosome. In this study of Escherichia coli Pth, the critical role of histidine 20 was investigated by site-directed mutagenesis, stopped-flow kinetic measurements, and chemical modification. The histidine residue at position ... More
Potential treatment of cerebral global ischemia with Oct-4+ umbilical cord matrix cells.
AuthorsJomura S, Uy M, Mitchell K, Dallasen R, Bode CJ, Xu Y
JournalStem Cells
PubMed ID16960128
Potential therapeutic effects of Oct-4-positive rat umbilical cord matrix (RUCM) cells in treating cerebral global ischemia were evaluated using a reproducible model of cardiac arrest (CA) and resuscitation in rats. Animals were randomly assigned to four groups: A, sham-operated; B, 8-minute CA without pretreatment; C, 8-minute CA pretreated with defined ... More
Optoinjection for efficient targeted delivery of a broad range of compounds and macromolecules into diverse cell types.
AuthorsClark IB, Hanania EG, Stevens J, Gallina M, Fieck A, Brandes R, Palsson BO, Koller MR
JournalJ Biomed Opt
PubMed ID16526911
Efficient delivery of compounds and macromolecules into living cells is essential in many fields including basic research, applied drug discovery, and clinical gene therapy. Unfortunately, current delivery methods, such as cationic lipids and electroporation, are limited by the types of macromolecules and cells that can be employed, poor efficiency, and/or ... More
Analysis of the ataxia telangiectasia mutated-mediated DNA damage response in murine cerebellar neurons.
AuthorsDar I, Biton S, Shiloh Y, Barzilai A
JournalJ Neurosci
PubMed ID16855104
The DNA damage response is a network of signaling pathways that affects many aspects of cellular metabolism after the induction of DNA damage. The primary transducer of the cellular response to the double-strand break, a highly cytotoxic DNA lesion, is the nuclear protein kinase ataxia telangiectasia (A-T) mutated (ATM), which ... More
Role of Dicer and Drosha for endothelial microRNA expression and angiogenesis.
AuthorsKuehbacher A, Urbich C, Zeiher AM, Dimmeler S
JournalCirc Res
PubMed ID17540974
MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression by binding to the cellular transcript leading to translational repression or degradation of the target mRNA. Dicer and Drosha are the miRNA processing enzymes that are required for the maturation of miRNAs. Here, we investigated the role of Dicer and ... More
Increase in cytosolic Ca2+ levels through the activation of non-selective cation channels induced by oxidative stress causes mitochondrial depolarization leading to apoptosis-like death in Leishmania donovani promastigotes.
AuthorsMukherjee SB, Das M, Sudhandiran G, Shaha C
JournalJ Biol Chem
PubMed ID11983701
Reactive oxygen species are important regulators of protozoal infection. Promastigotes of Leishmania donovani, the causative agent of Kala-azar, undergo an apoptosis-like death upon exposure to H2O2. The present study shows that upon activation of death response by H2O2, a dose- and time-dependent loss of mitochondrial membrane potential occurs. This loss ... More
Lineage-specific enhancers activate self-renewal genes in macrophages and embryonic stem cells.
Authors
JournalScience
PubMed ID26797145
Dynamic visualization of the recovery of mouse hepatobiliary metabolism to acetaminophen-overdose damage.
AuthorsLi FC, Lee SL, Lin HM, Lin CJ, Wang SS, Lee YY, Lo SY, Sun TL, Chen WL, Lo W, Horton N, Xu C, Chiang SJ, Chen YF, Lee HS, Dong CY
JournalJ Biophotonics
PubMed ID30302934
'Acetaminophen (APAP) overdose is one of the world''s leading causes of drug-induced hepatotoxicity. Although traditional methods such as histological imaging and biochemical assays have been successfully applied to evaluate the extent of APAP-induced liver damage, detailed effect of how APAP overdose affect the recovery of hepatobiliary metabolism and is not ... More
Elucidation of a sialic acid metabolism pathway in mucus-foraging Ruminococcus gnavus unravels mechanisms of bacterial adaptation to the gut.
AuthorsBell A, Brunt J, Crost E, Vaux L, Nepravishta R, Owen CD, Latousakis D, Xiao A, Li W, Chen X, Walsh MA, Claesen J, Angulo J, Thomas GH, Juge N
JournalNat Microbiol
PubMed ID31636419
'Sialic acid (N-acetylneuraminic acid (Neu5Ac)) is commonly found in the terminal location of colonic mucin glycans where it is a much-coveted nutrient for gut bacteria, including Ruminococcus gnavus. R. gnavus is part of the healthy gut microbiota in humans, but it is disproportionately represented in diseases. There is therefore a ... More
Treatment with mRNA coding for the necroptosis mediator MLKL induces antitumor immunity directed against neo-epitopes.
Authors
JournalNat Commun
PubMed ID30143632
Targeted immunotherapy for HER2-low breast cancer with 17p loss.
Authors
JournalSci Transl Med
PubMed ID33568521
Specifically differentiated T cell subset promotes tumor immunity over fatal immunity.
Authors
JournalJ Exp Med
PubMed ID29038366
Evaluating totipotency using criteria of increasing stringency.
Authors
JournalNat Cell Biol
PubMed ID33420491
Engineering of a bona fide light-operated calcium channel.
Authors
JournalNat Commun
PubMed ID33431868
Colorectal Cancer Cells Enter a Diapause-like DTP State to Survive Chemotherapy.
Authors
JournalCell
PubMed ID33417860
Cancer cells dying from ferroptosis impede dendritic cell-mediated anti-tumor immunity.
Authors
JournalNat Commun
PubMed ID35760796
Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS.
Authors
JournalNat Protoc
PubMed ID31996843
Cytoplasmic condensation induced by membrane damage is associated with antibiotic lethality.
Authors
JournalNat Commun
PubMed ID33875652
Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche.
AuthorsBalzano M, De Grandis M, Vu Manh TP, Chasson L, Bardin F, Farina A, Sergé A, Bidaut G, Charbord P, Hérault L, Bailly AL, Cartier-Michaud A, Boned A, Dalod M, Duprez E, Genever P, Coles M, Bajenoff M, Xerri L, Aurrand-Lions M, Schiff C, Mancini SJC
JournalCell Rep
PubMed ID30893599
In the bone marrow, CXCL12 and IL-7 are essential for B cell differentiation, whereas hematopoietic stem cell (HSC) maintenance requires SCF and CXCL12. Peri-sinusoidal stromal (PSS) cells are the main source of IL-7, but their characterization as a pro-B cell niche remains limited. Here, we characterize pro-B cell supporting stromal cells ... More
Immunodominant AH1 Antigen-Deficient Necroptotic, but Not Apoptotic, Murine Cancer Cells Induce Antitumor Protection.
AuthorsAaes TL, Verschuere H, Kaczmarek A, Heyndrickx L, Wiernicki B, Delrue I, De Craene B, Taminau J, Delvaeye T, Bertrand MJM, Declercq W, Berx G, Krysko DV, Adjemian S, Vandenabeele P
JournalJ Immunol
PubMed ID31900335
Immunogenic cell death (ICD) occurs when a dying cell releases cytokines and damage-associated molecular patterns, acting as adjuvants, and expresses Ags that induce a specific antitumor immune response. ICD is studied mainly in the context of regulated cell death pathways, especially caspase-mediated apoptosis marked by endoplasmic reticulum stress and calreticulin ... More
Host Lymphotoxin-ß Receptor Signaling Is Crucial for Angiogenesis of Metanephric Tissue Transplanted into Lymphoid Sites.
AuthorsFrancipane MG, Han B, Lagasse E
JournalAm J Pathol
PubMed ID31585070
The mouse lymph node (LN) can provide a niche to grow metanephric kidney to maturity. Here, we show that signaling through the lymphotoxin-ß receptor (LTßR) is critical for kidney organogenesis both in the LN and the omentum. By transplanting kidney rudiments either in the LNs of mice undergoing LTßR antagonist ... More