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Citations & References (7)
Invitrogen™
pAc5.1/V5-His A, B, & C Vectors
pAC5.1/V5-His vectors are designed for use in Drosophila cells to achieve high-level transient expression of recombinant proteins. The vector offersRead more
| Catalog Number | Quantity |
|---|---|
| V411020 | 20 μg |
Catalog number V411020
Price (EUR)
1 176,00
20 µg
Quantity:
20 μg
Price (EUR)
1 176,00
20 µg
pAC5.1/V5-His vectors are designed for use in Drosophila cells to achieve high-level transient expression of recombinant proteins. The vector offers the strong, constitutive promoter from the Drosophila actin 5C gene. The pAC5.1/V5-His vectors can be used with the DES™-Inducible Kits (K512001 and K412001) for constitutive expression of your protein of interest. The pAc5.1/V5-His vectors also offer the following features:
• Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency
• C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
• C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag.
• Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency
• C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
• C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatLyophilized
Product TypeDrosophila Expression Vector
Quantity20 μg
VectorpAC
Cloning MethodRestriction Enzyme/MCS
PromoterpAC5
Protein TagHis Tag (6x), V5 Epitope Tag
Unit Size20 µg
Contents & Storage
Contains 20 µg each of lyophilized pAc5.1/V5-His A, B, C and pAc5.1/V5-His/LacZ.
Store at -20°C. All components are guaranteed stable for 6 months when properly stored.
Store at -20°C. All components are guaranteed stable for 6 months when properly stored.
Citations & References (7)
Citations & References
Abstract
Gata factor translation is the final downstream step in the amino acid/tor-mediated vitellogenin gene expression in the anautogenous mosquito aedes aegypti.
Journal:J Biol Chem
PubMed ID:16490782
'Ingestion of blood is required for vector mosquitoes to initiate reproductive cycles determining their role as vectors of devastating human diseases. Nutritional signaling plays a pivotal role in regulating mosquito reproduction. Transcription of yolk protein precursor genes is repressed until mosquitoes take blood. Previously, we have shown that to signal
Inwardly rectifying K+ (Kir) channels in Drosophila. A crucial role of cellular milieu factors Kir channel function.
Journal:J Biol Chem
PubMed ID:11964404
'Three cDNAs encoding inwardly rectifying potassium (Kir) channels were isolated from Drosophila melanogaster. The protein sequences of Drosophila KirI (dKirI) and dKirII are moderately (<44%) and dKirIII sequence is weakly (<27%) identical to human Kir channel subunits. During fly development, five dKir channel transcripts derived from three genes are differentially
Methylation-mediated transcriptional silencing in euchromatin by methyl-CpG binding protein MBD1 isoforms.
Journal:Mol Cell Biol
PubMed ID:10454587
DNA methylation of promoter-associated CpG islands is involved in the transcriptional repression of vertebrate genes. To investigate the mechanisms underlying gene inactivation by DNA methylation, we characterized a human MBD1 protein, one of the components of MeCP1, which possesses a methyl-CpG binding domain (MBD) and cysteine-rich (CXXC) domains. Four novel
A baculovirus superinfection system: efficient vehicle for gene transfer into drosophila S2 cells.
Journal:J Virol
PubMed ID:11090187
The baculovirus expression vector system is considered to be a safe, powerful, but cell-lytic heterologous protein expression system in insect cells. We show here that there is a new baculovirus system for efficient gene transfer and expression using the popular and genetically well-understood Drosophila S2 cells. The recombinant baculovirus was
The eukaryotic DNMT2 genes encode a new class of cytosine-5 DNA methyltransferases.
Journal:J Biol Chem
PubMed ID:12819212
DNMT2 is a subgroup of the eukaryotic cytosine-5 DNA methyltransferase gene family. Unlike the other family members, proteins encoded by DNMT2 genes were not known before to possess DNA methyltransferase activities. Most recently, we have shown that the genome of Drosophila S2 cells stably expressing an exogenous Drosophila dDNMT2 cDNA