PBS, pH de 7,4
Gibco™

PBS, pH de 7,4

La solution PBS (solution saline dans un tampon phosphate) est une solution saline équilibrée utilisée dans une variété d’applications deAfficher plus
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RéférenceQuantité
10010023500 mL
100100311 000 mL
Référence 10010023
Prix (EUR)
42,65
線上優惠
47,94
Économisez 5,29 (11%)
Each
Quantité:
500 mL
Prix (EUR)
42,65
線上優惠
47,94
Économisez 5,29 (11%)
Each
La solution PBS (solution saline dans un tampon phosphate) est une solution saline équilibrée utilisée dans une variété d’applications de culture cellulaire telles que le lavage des cellules avant dissociation, le transport des cellules et des tissus, la dilution des cellules pour comptage et la préparation des réactifs. La solution PBS est formulée sans calcium ni magnésium pour rincer les chélateurs de la culture avant la dissociation des cellules. Nous proposons une variété de formulations de PBS pour une gamme d’applications de culture cellulaire. Trouvez la formulation la mieux adaptée à l’aide de l’outil de sélection de milieux de culture.

Cette solution PBS est fabriquée comme suit :
Sans
• Calcium
• Magnésium
• Rouge de phénol

La formulation complète est disponible.

Système de fabrication et de Qualité BPFa
PBS est fabriqué dans un établissement conforme aux BPFa, situé à Paisley, en Écosse, au Royaume-Uni. Ce site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485. Pour assurer la continuité de la chaîne d’approvisionnement, nous proposons un produit identique à la solution PBS fabriquée sur notre site de Grand Island (10010015). Ce site est également homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485.
À des fins de recherche ou de commercialisation avancée Non destiné aux diagnostics ou à l’administration directe à des humains ou des animaux.
Spécifications
Nom chimique ou matériauSolution saline dans un tampon phosphate
CouleurTransparents
ComprendNo Calcium, No Magnesium
Qualité de fabricationcGMP-compliant under the ISO 13485 standard
Osmolalité280 à 315 mOsm/kg
ConditionnementBottle
Température de stockageConditions de stockage : 15°C à 30°C
Conditions d’expédition : température ambiante
Durée de conservation : 24 mois à compter de la date de fabrication
StérilitéStérilisation par filtration
Concentration1 X
À utiliser avec (application)Lavage des cellules avant dissociation ; dilution des cellules pour le comptage et la préparation des réactifs ; transport des cellules ou des tissus
Forme physiqueLiquide
Gamme de produitsGibco
Quantité500 mL
Type de solutionSolution saline dans un tampon phosphate
pH7,4
Unit SizeEach

Foire aux questions (FAQ)

How can I make isolation buffer for cell isolation?

To prepare this buffer:

(1) Make up 100 mL of a stock solution of 0.5 M EDTA by dissolving 14.6 g EDTA in approximately 80 mL of ddH2O. Adjust the pH to 7.4, then adjust the volume to 100 mL. Autoclave and filter 25 mL aliquots through a sterilized filter into 4 sterile 50 mL tubes. Store one at 4 degrees C and the rest at -20 degrees C for future use.
(2) Make up a stock solution of 20% BSA by putting 5 g of BSA in a 50 mL tube and filling it with ddH2O up to 25 mL. Leave on a roller at room temperature until dissolved. Filter through a sterile 0.2 µm filter into a sterile 50 mL tube and adjust the volume to 30 mL with sterile H2O.
(3) Make the isolation buffer by taking a 500 mL bottle of 1x PBS, pH 7.4 without Ca2+ or Mg2+ (Cat. No. 10010023). Add 2.5 mL 20% BSA and 2 mL 0.5 M EDTA. Mix by turning the bottle end over end 10 times. Store at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My PBS, PH 7.4 (Cat. No. 10010072) was accidentally frozen. Can I still use it?

This product should be stored at 2-8 degrees C and should not be frozen. The biggest issue with accidentally freezing this product is its solubility. If this product was accidentally frozen, we recommend placing it in a 2-8 degree environment and allowing it to slowly thaw overnight. If the product is fully thawed and shows no signs of precipitation, then it should still be usable, but we cannot guarantee effectiveness.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your PBS products tested for endotoxin levels?

Our PBS, pH 7.2 and PBS, pH 7.4 products are tested for endotoxin and the endotoxin levels are shown on the Certificate of Analysis (CoA) for the particular lot number of the product.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your PBS products sterile and suitable for cell culture?

We offer PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free (Cat. Nos. AM9624 and AM9625), which is molecular biology grade and is certified to be RNase-free. However, it is not sterile and hence is not cell culture-grade.

All our other PBS products (Cat. Nos. 70011xxx, 10010xxx, 70013xxx, 20012xxx) are filter-sterilized using a 0.1 µm filter and are cell culture grade (ready to use directly on cells). However, they are not tested for nuclease activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your PBS products DNase- and RNase-free?

We offer PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free (Cat. Nos. AM9624 and AM9625) that is molecular biology grade and is certified to be RNase-free. Our other PBS products are made with distilled water and are filter-sterilized, but are not tested for RNase and DNase activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations et références (6)

Citations et références
Abstract
A comprehensive protocol for efficient differentiation of human NPCs into electrically competent neurons.
Authors:Romito E,Battistella I,Plakhova V,Paplekaj A,Forastieri C,Toffolo E,Musio C,Conti L,Battaglioli E,Rusconi F
Journal:Journal of neuroscience methods
PubMed ID:39053772
Aldose reductase mediates mitogenic signaling in vascular smooth muscle cells.
Authors: Ramana Kota V; Chandra Deepak; Srivastava Sanjay; Bhatnagar Aruni; Aggarwal Bharat B; Srivastava Satish K;
Journal:J Biol Chem
PubMed ID:12063254
'Abnormal vascular smooth muscle cell (VSMC) proliferation is a key feature of atherosclerosis and restenosis; however, the mechanisms regulating growth remain unclear. Herein we show that inhibition of the aldehyde-metabolizing enzyme aldose reductase (AR) inhibits NF-kappa B activation during restenosis of balloon-injured rat carotid arteries as well as VSMC proliferation ... More
Blocking HES1 expression initiates GABAergic differentiation and induces the expression of p21(CIP1/WAF1) in human neural stem cells.
Authors: Kabos Peter; Kabosova Andrea; Neuman Toomas;
Journal:J Biol Chem
PubMed ID:11809764
Mammalian neural stem cells can develop into a variety of neuronal and glial cell types. This involves a highly coordinated process of differentiation in which the Notch signaling pathway and the system of helix-loop-helix (HLH) transcriptional regulators play a key role. By exercising control over proliferation, initiation of differentiation, neurite ... More
Cholesterol modulates the membrane binding and intracellular distribution of annexin 6.
Authors: de Diego Iñaki; Schwartz Felix; Siegfried Heide; Dauterstedt Paul; Heeren Joerg; Beisiegel Ulrike; Enrich Carlos; Grewal Thomas;
Journal:J Biol Chem
PubMed ID:12070178
Annexins are Ca(2+)- and phospholipid-binding proteins that are widely expressed in mammalian tissues and that bind to different cellular membranes. In recent years its role in membrane traffic has emerged as one of its predominant functions, but the regulation of its intracellular distribution still remains unclear. We demonstrated that annexin ... More
A 2-D liquid separations/mass mapping method for interlysate comparison of ovarian cancers.
Authors: Kachman Maureen T; Wang Haixing; Schwartz Donald R; Cho Kathleen R; Lubman David M;
Journal:Anal Chem
PubMed ID:11985308
A two-dimensional liquid phase separation of proteins from whole cell lysates coupled on-line to an electrospray-ionization time-of-flight (ESI-TOF) mass spectrometer (MS) is used to map the protein content of ovarian surface epithelial cells (OSE) and an ovarian carcinoma-derived cell line (ES2). The two dimensions involve the use of liquid isoelectric ... More