Citations et références (4)

Invitrogen™

ADN polymérase Taq, recombinante

Name: ADN polymérase Taq , recombinante
SKU: 10342020
Price: 385.00 EUR

L’ADN polymérase Taq est une enzyme thermostable, qui permet de synthétiser l’ADN des modèles premier brins en présence de dNTPAfficher plus

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Référence	Nbre de réactions
10342020	500 réactions
10342053	100 réactions
10342046	1500 réactions
10342178	5000 réactions

4 options

Référence 10342020

Prix (EUR)

385,00

Each

Nbre de réactions:

500 réactions

Grand volume ou format personnalisé

Prix (EUR)

385,00

Each

L’ADN polymérase Taq est une enzyme thermostable, qui permet de synthétiser l’ADN des modèles premier brins en présence de dNTP et d’une amorce. Cette enzyme est constituée d’un polypeptide unique, dont le poids moléculaire est de 94 kDa. Elle présente une activité d’ADN polymérase 5´→3´ et une activité d’exonucléase 5´→3´

Avec notre ADN polymérase Taq, vous obtenez :

• Votre choix d’enzymes recombinantes ou natives
• Amplification des produits PCR jusqu’à 5 kb
• Une enzyme sous licence et qualifiée pour PCR

Applications
L’ADN polymérase Taq convient à l’amplification d’ADN provenant de génomiques complexes, de modèles viraux et plasmidiques, de RT-PCR, le séquençage de l’ADNsb et le séquençage cyclique.

Source
L’enzyme recombinante est purifiée à partir du gène cloné de l’ADN polymérase Thermus aquaticus exprimé dans E. coli.

Définition d’unité
Une unité d’ADN polymérase Taq est la quantité d’enzyme nécessaire pour incorporer 10 nmol de désoxyribonucléotide dans l’ADN en 30 min à 74°C.

Pour des performances PCR supérieures, nous recommandons l’ADN polymérase DreamTaq.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Activité exonucléasique5’ à 3’

Fidélité (par rapport à Taq)1 X

FormatEnzyme autonome

Hot StartNon

Nbre de réactions500 réactions

En surplomb3’-A

PolyméraseADN polymérase Taq

Type de produitADN polymérase

Quantité500 unités

Format de réactionComposants séparés

Conditions d’expéditionHomologué pour des expéditions sur de la glace carbonique ou mouillée

Taille (produit final)5 kb ou moins

Matériau de départADN

Concentration5 U/μl

À utiliser avec (application)Standard PCR

GC-Rich PCR PerformanceFaible

Méthode PCRqPCR, PCR standard

Vitesse de réactionÉtalon

Unit SizeEach

Contenu et stockage

Contenu :
• 100 μl d’ADN polymérase Taq (5 U/μl)
• 2,5 ml de tampon de PCR 10X (200 mM de Tris-HCl pH 8,4, 500 mM de KCl)
• 1 ml de chlorure de magnésium (50 mM)

Conserver entre -30°C et -10°C dans un congélateur sans système de dégivrage. Garantie stable pendant 6 mois sous réserve d’un stockage correct.

Foire aux questions (FAQ)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

View all ADN polymérase Taq, recombinante FAQs

Citations et références (4)

Citations et références

Abstract

alpha-Methylacyl coenzyme A racemase as a tissue biomarker for prostate cancer.

Authors: Rubin Mark A; Zhou Ming; Dhanasekaran Saravana M; Varambally Sooryanarayana; Barrette Terrence R; Sanda Martin G; Pienta Kenneth J; Ghosh Debashis; Chinnaiyan Arul M;

Journal:JAMA

PubMed ID:11926890

'Edited due to space constraints: CONTEXT: Molecular profiling of prostate cancer has led to the identification of candidate biomarkers and regulatory genes. Discoveries from these genome-scale approaches may have applicability in the analysis of diagnostic prostate specimens. OBJECTIVES: To determine the expression and clinical utility of alpha-methylacyl coenzyme A racemase ... More

Novel isoforms of the sodium channels Nav1.8 and Nav1.5 are produced by a conserved mechanism in mouse and rat.

Authors:Kerr NC, Holmes FE, Wynick D,

Journal:J Biol Chem

PubMed ID:15047701

The voltage-gated sodium channel Nav1.8 is only expressed in subsets of neurons in dorsal root ganglia (DRG), trigeminal and nodose ganglia. We have isolated mouse partial-length Nav1.8 cDNA clones spanning exon 17 sequence which have 17 nucleotide substitutions and 12 predicted amino acid differences from the published sequence. The absence ... More

Low density lipoprotein receptor-related protein mediates apolipoprotein E inhibition of smooth muscle cell migration.

Authors: Swertfeger Debi K; Bu Guojun; Hui David Y;

Journal:J Biol Chem

PubMed ID:11739389

This research was undertaken to identify the cell surface receptor responsible for mediating apolipoprotein E (apoE) inhibition of platelet-derived growth factor (PDGF)-directed smooth muscle cell migration. Initial studies revealed the expression of the low density lipoprotein receptor (LDLR), the LDL receptor-related protein (LRP), the very low density lipoprotein receptor (VLDL), ... More

Association between CYP2C9 genetic variants and anticoagulation-related outcomes during warfarin therapy.

Authors: Higashi Mitchell K; Veenstra David L; Kondo L Midori; Wittkowsky Ann K; Srinouanprachanh Sengkeo L; Farin Fred M; Rettie Allan E;

Journal:JAMA

PubMed ID:11926893

Edited for space constraints: Warfarin is a commonly used anticoagulant that requires careful clinical management to balance the risks of overanticoagulation and bleeding with those of underanticoagulation and clotting. The principal enzyme involved in warfarin metabolism is CYP2C9, and 2 relatively common variant forms with reduced activity have been identified, ... More