ADN polymérase Taq, recombinante
ADN polymérase <i>Taq</i>, recombinante
ADN polymérase <i>Taq</i>, recombinante
ADN polymérase <i>Taq</i>, recombinante
Citations et références (3)
Invitrogen™

ADN polymérase Taq, recombinante

L’ADN polymérase Taq est une enzyme thermostable, qui permet de synthétiser l’ADN des modèles premier brins en présence de dNTPAfficher plus
Have Questions?
Modifier l'affichagebuttonViewtableView
RéférenceNbre de réactions
103420461500 réactions
10342053100 réactions
10342020500 réactions
103421785000 réactions
Référence 10342046
Prix (EUR)
1 006,00
Each
Ajouter au panier
Nbre de réactions:
1500 réactions
Grand volume ou format personnalisé
Prix (EUR)
1 006,00
Each
Ajouter au panier
L’ADN polymérase Taq est une enzyme thermostable, qui permet de synthétiser l’ADN des modèles premier brins en présence de dNTP et d’une amorce. Cette enzyme est constituée d’un polypeptide unique, dont le poids moléculaire est de 94 kDa. Elle présente une activité d’ADN polymérase 5´→3´ et une activité d’exonucléase 5´→3´

Avec notre ADN polymérase Taq, vous obtenez :

• Votre choix d’enzymes recombinantes ou natives
• Amplification des produits PCR jusqu’à 5 kb
• Une enzyme sous licence et qualifiée pour PCR

Applications
L’ADN polymérase Taq convient à l’amplification d’ADN provenant de génomiques complexes, de modèles viraux et plasmidiques, de RT-PCR, le séquençage de l’ADNsb et le séquençage cyclique.

Source
L’enzyme recombinante est purifiée à partir du gène cloné de l’ADN polymérase Thermus aquaticus exprimé dans E. coli.

Définition d’unité
Une unité d’ADN polymérase Taq est la quantité d’enzyme nécessaire pour incorporer 10 nmol de désoxyribonucléotide dans l’ADN en 30 min à 74°C.

Pour des performances PCR supérieures, nous recommandons l’ADN polymérase DreamTaq.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Activité exonucléasique5’ à 3’
Fidélité (par rapport à Taq)1 X
FormatEnzyme autonome
Hot StartNon
Nbre de réactions1500 réactions
En surplomb3’-A
PolyméraseADN polymérase Taq
Type de produitADN polymérase
Quantité3 x 500 unités
Format de réactionComposants séparés
Conditions d’expéditionDry Ice
Taille (produit final)5 kb ou moins
Matériau de départADN
Concentration5 U/μl
À utiliser avec (application)Standard PCR
GC-Rich PCR PerformanceFaible
Méthode PCRqPCR, PCR standard
Vitesse de réactionÉtalon
Unit SizeEach
Contenu et stockage
• Taq DNA Polymerase (5 U/μL), 3 x 100 μL
• 10X PCR buffer, 7.5 mL
• 50 mM MgCl2, 3 mL

Store at -10°C to -30°C in a non-frost-free freezer.
Guaranteed stable for 6 months when stored properly.

Foire aux questions (FAQ)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

View all ADN polymérase Taq, recombinante FAQs

Citations et références (3)

Citations et références
Abstract
alpha-Methylacyl coenzyme A racemase as a tissue biomarker for prostate cancer.
Authors: Rubin Mark A; Zhou Ming; Dhanasekaran Saravana M; Varambally Sooryanarayana; Barrette Terrence R; Sanda Martin G; Pienta Kenneth J; Ghosh Debashis; Chinnaiyan Arul M;
Journal:JAMA
PubMed ID:11926890
'Edited due to space constraints: CONTEXT: Molecular profiling of prostate cancer has led to the identification of candidate biomarkers and regulatory genes. Discoveries from these genome-scale approaches may have applicability in the analysis of diagnostic prostate specimens. OBJECTIVES: To determine the expression and clinical utility of alpha-methylacyl coenzyme A racemase ... More
Molecular cross-talk between the TRAIL and interferon signaling pathways.
Authors: Kumar-Sinha Chandan; Varambally Sooryanarayana; Sreekumar Arun; Chinnaiyan Arul M;
Journal:J Biol Chem
PubMed ID:11677236
TRAIL/APO-2L induces apoptosis in a variety of transformed cells and has potential as an anti-cancer therapeutic. The physiologic role of TRAIL is presumably more complex than merely activating caspase-mediated cell death. To shed light into TRAIL-mediated signaling, we used DNA microarrays to profile gene expression mediated by TRAIL in breast ... More
Association between CYP2C9 genetic variants and anticoagulation-related outcomes during warfarin therapy.
Authors: Higashi Mitchell K; Veenstra David L; Kondo L Midori; Wittkowsky Ann K; Srinouanprachanh Sengkeo L; Farin Fred M; Rettie Allan E;
Journal:JAMA
PubMed ID:11926893
Edited for space constraints: Warfarin is a commonly used anticoagulant that requires careful clinical management to balance the risks of overanticoagulation and bleeding with those of underanticoagulation and clotting. The principal enzyme involved in warfarin metabolism is CYP2C9, and 2 relatively common variant forms with reduced activity have been identified, ... More