Hygromycine B (50 mg/ml)
Hygromycine B (50 mg/ml)
Citations et références (38)
Gibco™

Hygromycine B (50 mg/ml)

L’hygromycine B est un antibiotique hydrosoluble obtenu par purification de la bactérie Streptomyces hydroscopicus. L’antibiotique Gibco™ Hygromycin B est utiliséAfficher plus
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1068701020 ml
Référence 10687010
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376,65
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384,00
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Quantité:
20 ml
Prix (EUR)
376,65
Online Exclusive
384,00
Économisez 7,35 (2%)
Each
Ajouter au panier
L’hygromycine B est un antibiotique hydrosoluble obtenu par purification de la bactérie Streptomyces hydroscopicus. L’antibiotique Gibco™ Hygromycin B est utilisé comme antibiotique de sélection des cellules bactériennes dans la plage de concentrations comprise entre 200 et 500 μg / ml. Ce produit est fourni sous forme de solution de 50 mg / ml.

La résistance à l’hygromycine B est conférée par le gène de résistance à l’hygromycine E. coli (hyg ou hph). L’hygromycine B présente un mode d’action différent des réactifs de sélection de Geneticin™, Blasticidin S ou Zeocin™, ce qui en fait un excellent choix pour des expériences à double sélection.(voir les antibiotiques de sélection).

Autres choix et informations complémentaires
Nous proposons une large gamme d’antibiotiques et d’antimycosiques en formats poudre et liquide.

Consultez la liste complèteou recherchez des produits pour :
• Contrôle de la contamination
• Sélection eucaryote et bactérienne

Voir les recommandations pour les concentrations de travail pour les antibiotiques de sélection.

En savoir plus sur l’utilisation d’antibiotiques et d’antimycotiques dans la culture cellulaire et consulter les directives pour la décontamination des cultures.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Concentration50 mg/ml
Type de cultureCulture de cellules de mammifères, culture de cellules d’insectes
À utiliser avec (application)Sélection eucaryote ⁄ génération de lignées cellulaires stables
Quantité20 ml
Conditions d’expéditionTempérature ambiante
FormeLiquide
Type de produitAntibiotique
StérilitéStérile
Unit SizeEach
Contenu et stockage
Conditions de stockage : 2 à 8°C
Conditions d’expédition : Température ambiante

Foire aux questions (FAQ)

Which of your antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for stable selection in mammalian cells?

All of our antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for making multiple stable cell lines. However, kill curves will need to be performed for each combination of antibiotics since sensitivity to a given antibiotic tends to increase when combined with other antibiotics.

What are the recommended concentrations of antibiotics to use for selection in prokaryotes and eukaryotes?

For best results, optimal concentrations for selection should be determined empirically in each unique experiment through dose response curves. However, to get a general idea of concentrations that have worked for individual cell types, please click on the following url: http://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/selection.html or type in “Selection Antibiotics” into our main search on www.thermofisher.com.

What is the range of specific activity of Hygromycin B?

The specific activity range of Hygromycin B is from 386-482 micrograms/mg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What solvent is used to prepare your Hygromycin B solution? How should it be stored?

Hygromycin B is supplied as a 50 mg/mL solution in phosphate buffered saline. It is manufactured aseptically and sterile filtered, and is suitable for use in tissue culture applications. We recommend storing it at 2-8 degrees C.

What is the mode of action on the following antibiotics: Blasticidin, Geneticin (G418), Hygromycin, and Zeocin?

Blasticidin: Nucleoside Inhibits protein synthesis in prokaryotic and eukaryotic cells by interfering with peptidyl transfer reaction of protein synthesis, causing early termination of translation.

Geneticin (G418): Aminoglycoside Blocks protein synthesis in mammalian cells by interfering with ribosomal function.

Hygromycin: Aminocyclitol Inhibits protein synthesis by disrupting translocation and promoting mistranslation.

Zeocin: Intercalates with DNA and cleaves it.

View all Hygromycine B (50 mg/ml) FAQs

Citations et références (38)

Citations et références
Abstract
WAF1, a potential mediator of p53 tumor suppression.
Authors:el-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, Lin D, Mercer WE, Kinzler KW, Vogelstein B
Journal:Cell
PubMed ID:8242752
The ability of p53 to activate transcription from specific sequences suggests that genes induced by p53 may mediate its biological role as a tumor suppressor. Using a subtractive hybridization approach, we identified a gene, named WAF1, whose induction was associated with wild- type but not mutant p53 gene expression in ... More
CC chemokine receptor 5-mediated signaling and HIV-1 Co-receptor activity share common structural determinants. Critical residues in the third extracellular loop support HIV-1 fusion.
Authors:Alkhatib G, Ahuja SS, Light D, Mummidi S, Berger EA, Ahuja SK
Journal:J Biol Chem
PubMed ID:9242636
'There is a close correspondence between the ability of RANTES and macrophage inflammatory proteins 1alpha and 1beta to activate CC chemokine receptor 5 (CCR5) and the ability to inhibit CCR5-dependent membrane fusion mediated by the envelope glycoprotein of human immunodeficiency virus (HIV), type 1. This finding suggests that some of ... More
Dimerization of a selectin and its ligand stabilizes cell rolling and enhances tether strength in shear flow.
Authors: Ramachandran V; Yago T; Epperson T K; Kobzdej M M; Nollert M U; Cummings R D; Zhu C; McEver R P;
Journal:Proc Natl Acad Sci U S A
PubMed ID:11526223
'Selectins mediate rolling of leukocytes by rapid formation and dissociation of selectin-ligand bonds, which are assumed to require high mechanical strength to prevent premature dissociation by the forces applied in shear flow. This assumption is based largely on the observation that increasing wall shear stress increases only modestly the dissociation ... More
Reconstitution of stretch-activated cation channels by expression of the alpha-subunit of the epithelial sodium channel cloned from osteoblasts [published erratum appears in Proc Natl Acad Sci U S A 1997 Apr 15;94(8):4233]
Authors:Kizer N, Guo XL, Hruska K
Journal:Proc Natl Acad Sci U S A
PubMed ID:9023374
'Osteoblasts respond to repetitive strain by activating stretch- activated, nonselective cation channels (SA-CAT) and increasing matrix protein production. SA-CAT channels are thought to be responsible for mechano-transduction in osteoblasts, although the molecular identity of the SA-CAT channel has previously been unknown. We have demonstrated that both the UMR-106 osteoblast-like cell ... More
Genetic analysis of the herpes simplex virus type 1 UL20 protein domains involved in cytoplasmic virion envelopment and virus-induced cell fusion.
Authors:Melancon JM, Foster TP, Kousoulas KG,
Journal:J Virol
PubMed ID:15220406
'The herpes simplex virus type 1 UL20 protein (UL20p) is an important determinant for cytoplasmic virion morphogenesis and virus-induced cell fusion. To delineate the functional domains of the UL20 protein, we generated a panel of single and multiple (cluster) alanine substitutions as well as UL20p carboxyl-terminal truncations. The UL20 mutant ... More
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