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SuperScript V VILO Master Mix, 1250 rxns
Invitrogen™

SuperScript V VILO Master Mix, 1250 rxns

Invitrogen™ SuperScript™ V VILO™ Master Mix is a ready-to-use master mix designed for fast, sensitive, and reproducible cDNA synthesis in two-step RT-qPCR applications.
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RéférenceNbre de réactionsComprend
11800002500 ReactionsSuperScript V VILO Master Mix, supplied with No RT Control and Nuclease-Free Water
1180000150 ReactionsSuperScript V VILO Master Mix, supplied with No RT Control and Nuclease-Free Water
118000032000 ReactionsSuperScript V VILO Master Mix, supplied with No RT Control and Nuclease-Free Water
1180011150 ReactionsSuperScript V VILO Master Mix, supplied with No RT Control, ezDNase Master Mix and Nuclease-Free Water
11800112500 ReactionsSuperScript V VILO Master Mix, supplied with No RT Control, ezDNase Master Mix and Nuclease-Free Water
118001132000 ReactionsSuperScript V VILO Master Mix, supplied with No RT Control, ezDNase Master Mix and Nuclease-Free Water
118000041250 ReactionsSuperScript V VILO Master Mix
Référence 11800002
Prix (EUR)
6 380,00
Each
Nbre de réactions:
500 Reactions
Comprend:
SuperScript V VILO Master Mix, supplied with No RT Control and Nuclease-Free Water
Prix (EUR)
6 380,00
Each

Invitrogen™ SuperScript™ V VILO™ Master Mix is a ready-to-use master mix designed for fast, sensitive, and reproducible cDNA synthesis in two-step RT-qPCR applications. Powered by SuperScript™ V Reverse Transcriptase, the optimized master mix combines robust performance across a broad range of RNA samples with simplified reaction setup, enhanced reagent stability, and greater workflow flexibility. SuperScript V VILO Master Mix is also available with ezDNase Master Mix for fast and convenient genomic DNA removal prior to reverse transcription.

Features of SuperScript V VILO Master Mix include:

  • Broad RNA input range—supports sensitive cDNA synthesis from 0.1 pg to 5 μg of total RNA
  • Robust performance with challenging RNA—efficient cDNA synthesis from highly structured, GC-rich, degraded and inhibitor-containing RNA
  • Fast cDNA synthesis—complete reverse transcription in as little as 1-10 minutes
  • Enhanced reagent stability—supports ambient shipping and room-temperature storage for up to 1 month
  • Simple, flexible workflow—ready-to-use format simplifies reaction setup, while room-temperature assembly and extended stability of assembled reactions enable greater workflow flexibility

SuperScript V VILO Master Mix combines SuperScript V RT, RNase inhibitor, buffer, dNTPs, and an optimized blend of oligo(dT) and random primers in a single formulation, simplifying reaction setup and helping reduce variability. Its combination of fast cDNA synthesis, robust performance, and flexible room-temperature handling makes it well suited for routine, high-throughput, and automated RT-qPCR workflows.

SuperScript V VILO Master Mix with gDNA Removal allows streamlined genomic DNA removal step. The dsDNA-specific ezDNase enzyme removes contaminating genomic DNA in as little as 2 minutes without affecting RNA, further simplifying the two-step RT-qPCR workflow.

A DTT-free, animal origin-free formulation combined with reduced cold chain requirements further simplifies reagent handling and supports more sustainable workflows.

For Research Use Only. Not for use in diagnostic procedures.
Spécifications
Type de produit finalFirst-Strand cDNA
FormatMaster Mix
ComprendSuperScript V VILO Master Mix, supplied with No RT Control and Nuclease-Free Water
Inhibitor Tolerance (Simple)High
Nbre de réactions500 Reactions
Température de réaction optimale50°
Type de produitReverse Transcription Reagent
Type de réactifReverse Transcription
Transcriptase inverseSuperScript V
Activité de la ribonucléase HReduced
Sensibilité0.1 pg total RNA
Conditions d’expéditionDry Ice, Wet Ice, Ambient
Taille (produit final)Up to 10 kb
Matériau de départRNA
TechniqueReverse Transcription
ThermostabilitéUp to 65°
Concentration5X
Méthode de détectionqPCR, NGS, PCR
À utiliser avec (application)Gene Expression, Real Time PCR (qPCR), RT-PCR, cDNA Synthesis
FormeLiquid
GC-Rich PCR PerformanceHigh
Marqueur ou colorantNot Applicable
Méthode PCR2-step RT-qPCR, RT-PCR
Vitesse de réaction10 min.
Unit SizeEach
Contenu et stockage
• SuperScript V VILO Master Mix (2 x 1 mL)
• SuperScript V VILO No RT Control (1 mL)
• Nuclease-free water (1.25 mL)

Store all contents at:
• -20°C for long-term storage until expiry date
• +5°C for up to 5 months
• Room temperature for up to 1 month