Citations et références (5)

Gibco™

Sulfate de streptomycine

Le sulfate de streptomycine est un antibiotique hydrosoluble purifié à l’origine par la bactérie Streptomyces griseus. Le sulfate de streptomycineAfficher plus

Référence	Quantité
11860038	100 g

Référence 11860038

Prix (EUR)

154,00

Quantité:

100 g

Prix (EUR)

154,00

Le sulfate de streptomycine est un antibiotique hydrosoluble purifié à l’origine par la bactérie Streptomyces griseus. Le sulfate de streptomycine agit en se liant à la sous-unité 30S du ribosome bactérien, ce qui conduit à l’inhibition de la synthèse protéique et à la mort des bactéries sensibles. Le sulfate de streptomycine agit très activement contre les bactéries à gram-négatif avec une certaine activité contre les bactéries à gram-positif. Le sulfate de streptomycine Gibco™ est utilisé seul ou en association avec la pénicilline, un antibiotique hautement actif contre les bactéries à gram-positif, pour la prévention de la contamination bactérienne des cultures cellulaires. Les plages de concentration recommandées s’échelonnent entre 50 et 100 µg / ml. Nous proposons un grand choix d’antibiotiques et d’antimycotiques pour les applications de culture cellulaire.

Utilisation du produit
uniquement à des fins de recherche : Non destiné au diagnostic ou à l’usage thérapeutique chez l’animal ou l’humain.

Fabrication conforme aux BPFa sur deux sites
Afin de garantir la continuité de la chaîne d’approvisionnement, nous manufacturons le sulfate de streptomycine Gibco™ sur deux sites différents : un situé à Grand Island, État de New York, États-Unis, l’autre en Écosse, au Royaume-Uni. Les deux sites sont conformes aux BPFa, certifiés ISO 13485 et homologués par la FDA comme fabricants de dispositifs médicaux.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Concentration50 à 100 μg/ml

Type de cultureCulture de cellules de mammifères, culture de cellules d’insectes

À utiliser avec (application)Sélection bactérienne

Quantité100 g

Durée de conservation24 mois

Conditions d’expéditionTempérature ambiante

FormePoudre

Type de produitAntibiotique

Unit SizeEach

Contenu et stockage

Conditions de stockage : 2 à 8°C
Conditions d’expédition : température ambiante
Durée de conservation : 24 mois à compter de la date de fabrication

Foire aux questions (FAQ)

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations et références (5)

Citations et références

Abstract

The cyclin-dependent kinase inhibitor p21WAF1/Cip1 is an antiestrogen-regulated inhibitor of Cdk4 in human breast cancer cells.

Authors: Skildum Andrew J; Mukherjee Shibani; Conrad Susan E;

Journal:J Biol Chem

PubMed ID:11741909

'The MCF-7 cell line is a model of estrogen-dependent, antiestrogen-sensitive human breast cancer. Antiestrogen treatment of MCF-7 cells causes dramatic decreases in both Cdk4 and Cdk2 activities, which leads to a G(1) phase cell cycle arrest. In this report, we investigate the mechanism(s) by which Cdk4 activity is regulated in ... More

Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats.

Authors: Lund R D; Adamson P; SauvÃ© Y; Keegan D J; Girman S V; Wang S; Winton H; Kanuga N; Kwan A S; BeauchÃ¨ne L; Zerbib A; Hetherington L; Couraud P O; Coffey P; Greenwood J;

Journal:Proc Natl Acad Sci U S A

PubMed ID:11504951

'Royal College of Surgeons rats are genetically predisposed to undergo significant visual loss caused by a primary dysfunction of retinal pigment epithelial (RPE) cells. By using this model, we have examined the efficacy of subretinal transplantation of two independent human RPE cell lines each exhibiting genetic modifications that confer long-term ... More

Exercise-induced a-ketoglutaric acid stimulates muscle hypertrophy and fat loss through OXGR1-dependent adrenal activation.

Authors:Yuan Y, Xu P, Jiang Q, Cai X, Wang T, Peng W, Sun J, Zhu C, Zhang C, Yue D, He Z, Yang J, Zeng Y, Du M, Zhang F, Ibrahimi L, Schaul S, Jiang Y, Wang J, Sun J, Wang Q, Liu L, Wang S, Wang L, Zhu X, Gao P, Xi Q, Yin C, Li F, Xu G, Zhang Y, Shu G

Journal:EMBO J

PubMed ID:32104923

'Beneficial effects of resistance exercise on metabolic health and particularly muscle hypertrophy and fat loss are well established, but the underlying chemical and physiological mechanisms are not fully understood. Here, we identified a myometabolite-mediated metabolic pathway that is essential for the beneficial metabolic effects of resistance exercise in mice. We ... More

Human GBP1 is a microbe-specific gatekeeper of macrophage apoptosis and pyroptosis.

Authors:Fisch D, Bando H, Clough B, Hornung V, Yamamoto M, Shenoy AR, Frickel EM

Journal:EMBO J

PubMed ID:31268602

'The guanylate binding protein (GBP) family of interferon-inducible GTPases promotes antimicrobial immunity and cell death. During bacterial infection, multiple mouse Gbps, human GBP2, and GBP5 support the activation of caspase-1-containing inflammasome complexes or caspase-4 which trigger pyroptosis. Whether GBPs regulate other forms of cell death is not known. The apicomplexan ... More

Mycobacterial Mutagenesis and Drug Resistance Are Controlled by Phosphorylation- and Cardiolipin-Mediated Inhibition of the RecA Coprotease.

Authors:Wipperman MF, Heaton BE, Nautiyal A, Adefisayo O, Evans H, Gupta R, van Ditmarsch D, Soni R, Hendrickson R, Johnson J, Krogan N, Glickman MS

Journal:Mol Cell

PubMed ID:30174294

'Infection with Mycobacterium tuberculosis continues to cause substantial human mortality, in part because of the emergence of antimicrobial resistance. Antimicrobial resistance in tuberculosis is solely the result of chromosomal mutations that modify drug activators or targets, yet the mechanisms controlling the mycobacterial DNA-damage response (DDR) remain incompletely defined. Here, we ... More