Milieu AIM V™, liquide (niveau de recherche)
Gibco™

Milieu AIM V™, liquide (niveau de recherche)

Contient de la L-glutamine, du sulfate de streptomycine à 50 µg / ml et du sulfate de gentamicine à 10Afficher plus
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RéférenceQuantité
120550831 000 mL
12055091500 mL
Référence 12055083
Prix (EUR)
246,65
キャンペーン価格
268,00
Économisez 21,35 (8%)
Each
Quantité:
1 000 mL
Customize this product
Prix (EUR)
246,65
キャンペーン価格
268,00
Économisez 21,35 (8%)
Each
Contient de la L-glutamine, du sulfate de streptomycine à 50 µg / ml et du sulfate de gentamicine à 10 µg / ml.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Type de celluleMonocytes, cellules dendritiques, cellules T, hybridomes, PBMC, fibroblastes, macrophages, cellules de myélome, lymphocytes
Antibiotiques inclusSulfate de streptomycine, gentamicine
Gamme de produitsAIM V
Type de produitMilieu sans sérum (SFM)
Pureté ou qualitéNiveau de recherche
Quantité1 000 mL
Conditions d’expéditionTempérature ambiante
Culture TypeCulture de cellules de mammifères
FormeLiquide
Avec additifsGlutamine, Rouge de phénol, HSA
Unit SizeEach
Contenu et stockage
Stocker au réfrigérateur (2 – 8°C) à l’abri de la lumière.

Foire aux questions (FAQ)

What is the difference between AIM V Medium (Cat. Nos. 12055083, 12055091) and CTS AIM V SFM (Cat. Nos. 0870112DK, 0870112BK)?

The difference is in the intended usage of the two media. AIM V Medium (Cat. Nos. 12055083, 12055091) is for research use only whereas CTS AIM V SFM (Cat. Nos. 0870112DK, 0870112BK) is for research use or manufacturing of cell, gene, or tissue-based products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free medium would you recommend for culture and expansion of mouse T cells?

You can try using AIM V Medium (Cat. No. 12055083 or 12055091) for growth of mouse T cells. For mouse cells, we recommend adding 50 µM beta-mercaptoethanol (Cat. No. 21985023).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations et références (26)

Citations et références
Abstract
Low oxygen tension and autologous plasma enhance T-cell proliferation and CD49d expression density in serum-free media.
Authors: Haddad H; Papoutsakis E T;
Journal:Cytotherapy
PubMed ID:11953028
'Background As cellular immunotherapy with ex vivo expanded cells becomes more widely used to treat a variety of illnesses, optimization of culture parameters, to maximize cell production and function, is essential for continued success. The effects of reduced oxygen tension and autologous plasma on T-cell expansion, receptor expression, apoptosis, and ... More
Effects of interleukin 4 upon human tumoricidal cells obtained from patients bearing solid tumors.
Authors: Jadus M R; Good R W; Crumpacker D B; Yannelli J R;
Journal:J Leukoc Biol
PubMed ID:1991997
'The use of human interleukin 4 (IL4) in the generation of tumoricidal effector cells derived from cancer patients undergoing either interleukin 2/lymphokine activated killer cells (IL2/LAK) therapy or tumor derived activated cell (TDAC) therapy was examined in the present study. Human IL4 alone did not generate LAK cells from patients ... More
T(84) epithelial cells respond to 5-hydroxytryptamine when grown in serum-free media.
Authors: Burleigh D; Fernandes K; Perrett D;
Journal:Eur J Pharmacol
PubMed ID:10708712
The aim of this study was to establish the cause of insensitivity of T(84) human colonic epithelial cells to 5-hydroxytryptamine (5-HT). Monolayers of T(84) cells were placed in modified Ussing chambers for measurement of short-circuit current, an index of secretion. When grown in serum-supplemented media, T(84) cells gave secretory responses ... More
Generation of human autologous melanoma-specific cytotoxic T cells from tumor-involved lymph nodes.
Authors: Maeda K; Lafreniere R; McKinnon J G; Jerry L M;
Journal:Mol Biother
PubMed ID:1910625
Cytotoxic T lymphocytes (CTL) specific for autologous human melanoma have been successfully generated in vitro from tumor bearing lymph nodes without any stimulation by the autologous tumor. Tumor-involved lymph node cells (LNC) were cultured in serum free medium (AIM-V) containing 1,000 U/ml of recombinant interleukin-2. The best expansion and specific ... More
Extracellular mRNA induces dendritic cell activation by stimulating tumor necrosis factor-alpha secretion and signaling through a nucleotide receptor.
Authors: Ni Houping; Capodici John; Cannon Georgetta; Communi Didier; Boeynaems Jean-Marie; Karikó Katalin; Weissman Drew;
Journal:J Biol Chem
PubMed ID:11821398
We previously demonstrated that dendritic cell (DC) pulsing with antigen-encoded mRNA resulted in the loading of both major histocompatibility complex class I and II antigen presentation pathways and the delivery of an activation signal. Coculture of mRNA-pulsed DC with T cells led to the induction of a potent primary immune ... More