Milieu de culture à faible osmolalité sans tampon L-glutamine ou HEPES optimisé pour la croissance de cellules souches embryonnaires etAfficher plus
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Quantité
12660012
500 mL
Référence 12660012
Prix (EUR)
36,18
Each
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Quantité:
500 mL
Customize this product
Prix (EUR)
36,18
Each
Ajouter au panier
Milieu de culture à faible osmolalité sans tampon L-glutamine ou HEPES optimisé pour la croissance de cellules souches embryonnaires et pluripotentes induites humaines.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Type de celluleCellules souches (embryonnaires), cellules souches (iPS - souches pluripotentes induites)
Qualité de fabricationBPFa pour dispositifs médicaux, 21 CFR Part 820 et ISO 13485
Gamme de produitsGibco
Type de produitKnockOut DMEM / F-12
Quantité500 mL
Conditions d’expéditionTempérature ambiante
FormeLiquide
Avec additifsRouge de phénol
Sans additifSans glutamine, Sans HEPES
Unit SizeEach
Contenu et stockage
Stocker entre 2° et 8°C et à l’abri de la lumière.
Foire aux questions (FAQ)
What is the concentration of glucose in KnockOut DMEM/F-12 Medium?
KnockOut DMEM/F-12 Medium contains 3151.0 mg/L of glucose.
We do not offer DMEM/F12 medium without folic acid as a standard catalog product. However, our Customs group can make it for you as a custom media. If you are interested, please complete this Custom order inquiry form PDF (https://www.thermofisher.com/content/dam/LifeTech/global/applied-sciences/pdfs/Bioproduction/Gibco-Custom-Media-Buffers-Request-Form.pdf) and email it to custommedia@thermofisher.com. Our Customs group will review your requirements and get back to you within a couple of days with pricing, ordering information, and lead time. After that, you have the choice of proceeding with an official order or declining.
How do I characterize human embryonic stem ( ES) cells?
Human ES cells are generally characterized by their typical morphology (they grow as tightly packed clusters of small cells with high ratio of nucleus to cytoplasm); surface marker expression; RT-PCR detection of stem cell-specific gene expression (such as Oct3/4, Sox2, and Nanog); alkaline phosphatase staining, and telomerase activity assay. The most commonly used ES specific surface markers include stage-specific embryonic antigens SSEA-3 and SSEA-4 for human ES cells. Other ES-specific surface antigens also include TRA-1-60 and TRA-1-81. (Science 282:1145 (1998).
How are human embryonic stem (ES) cells derived?
Human ES cells are derived from human blastocyst inner cell masses, isolated by immunosurgery with rabbit antiserum to BeWO cells (a human trophoblast cell line) (Science 282:1145 (1998)).