Stealth RNAi™ siRNA Negative Control Lo GC

Citations et références (3)

Invitrogen™

Stealth RNAi™ siRNA Negative Control Lo GC

Les contrôles négatifs de siARN Stealth RNAi™ constituent un excellent moyen de mesurer l’effet de votre duplex expérimental de siARNAfficher plus

Référence	Quantité
12935200	250 μlitres

Référence 12935200

Prix (EUR)

324,65

Online Exclusive

353,00

Économisez 28,35 (8%)

Ajouter au panier

Quantité:

250 μlitres

Prix (EUR)

324,65

Online Exclusive

353,00

Économisez 28,35 (8%)

Ajouter au panier

Les contrôles négatifs de siARN Stealth RNAi™ constituent un excellent moyen de mesurer l’effet de votre duplex expérimental de siARN Stealth RNAi™ par rapport au fond. Ces contrôles sont : –Conçus en 3 niveaux de teneur en GC (basse, moy. et haute) avec 3 séquences disponibles par niveau afin que les chercheurs puissent faire correspondre la teneur en GC à leur siARN expérimental Stealth RNAi™ –Non homologues à quoi que ce soit dans le transcriptome des vertébrés –Testé pour ne pas induire de réponse au stress pour les siARN Stealth RNAi™ Le kit de contrôle négatif contient les trois contrôles (teneur en GC basse, moy. et haute, à l’exception des séquences n 2 et n 3), chaque contrôle étant également disponible séparément. Obtenez des contrôles négatifs parfaits pour votre expérience RNAi afin de pouvoir évaluer vos résultats avec précision.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

À utiliser avec (application)ARNi, Transfection

Marqueur ou colorantNon conjugué(e)

Gamme de produitssiARN Stealth RNAi

Type de produitARNsi

Quantité250 μlitres

Type de contrôleContrôle négatif

RNAi TypesiARN

Unit SizeEach

Contenu et stockage

Fourni en format de 250 µl à 20 µM. Stocker à -20°C. Garanti stable pendant 6 mois sous réserve d’un stockage correct.

Foire aux questions (FAQ)

What are the benefits of using a vector to deliver RNAi?

Vector technologies allow you to:

Achieve transient or stable target knockdown
Perform RNAi in any cell type, even hard-to-transfect, primary, and non-dividing cells
Regulate gene inhibition with inducible siRNA expression
Select for a pure population of cells stably expressing an siRNA sequence
Control gene expression in vivo with tissue-specific promoters

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Why are my cells dying after transfection?

We would suggest running a transfection reagent control only to determine if your cells are sensitive to the transfection reagent. Additionally, you can try using different cell densities and siRNA concentrations to diminish any toxic effects from the transfection itself.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

I transfected my siRNA and the mRNA levels are down, but the protein is not. Why is that?

In some cases, knockdown of a protein can be affected by other variables such as protein turnover rate, even though the RNA is knocked down. Additionally, a longer time course may be needed to see an effect on protein compared to mRNA.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

I am not getting my target knockdown. What could be the cause of this?

Please see the following possibilities and suggestions:

- How many siRNA did you test? Is there any knockdown? If there is no knockdown (<10%) in any of the siRNA, then the assay is likely the problem. Try using a different qRT-PCR assay to assess knockdown.
- What was the positioning of the qRT-PCR assay target site relative to the cut site for the siRNA? If greater than 3,000 bases away, the problem could be alternative splice transcripts.
- What are the Cts for the experiment? They should be below 35 in a 40-cycle qRT-PCR experiment.
- Did you confirm the siRNA got into the cell? We recommend using a validated positive control siRNA to check the transfection efficiency.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

I am not getting any knockdown with my siRNA. What do you suggest I try?

Please see the following possibilities and suggestions:

- Were the mRNA levels checked? The most reliable method is real-time PCR. In some cases, knockdown of a protein can be affected by other variables, such as protein turnover rate, even though the RNA is knocked down.
- How is the RNA being isolated? Has the quality of the isolated RNA been checked? Ensure that the RNA has not been degraded.
- Was a positive control used? This can help to determine whether the reagents are working and whether the siRNA was delivered correctly to the cell. Run your experiment in parallel with the positive control siRNA.
- Was a transfection control used? What is the percentage of transfected cells?
- Was a time course used? Generally, gene silencing can be assessed as early as 24 hours posttransfection. However, the duration and level of knockdown are dependent on cell type and concentration of siRNA.
- Was optimization of transfection conditions performed? You can try using different cell densities and siRNA concentrations.
- Which concentration of siRNA did you use? We recommend testing multiple concentrations between 5 nM and 100 nM.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

View all Stealth RNAi™ siRNA Negative Control Lo GC FAQs

Citations et références (3)

Citations et références

Abstract

The Nck-interacting kinase phosphorylates ERM proteins for formation of lamellipodium by growth factors.

Authors:Baumgartner M, Sillman AL, Blackwood EM, Srivastava J, Madson N, Schilling JW, Wright JH, Barber DL,

Journal:Proc Natl Acad Sci U S A

PubMed ID:16938849

The mammalian Ste20-like Nck-interacting kinase (NIK) and its orthologs Misshapen in Drosophila and Mig-15 in Caenorhabditis elegans have a conserved function in regulating cell morphology, although through poorly understood mechanisms. We report two previously unrecognized actions of NIK: regulation of lamellipodium formation by growth factors and phosphorylation of the ERM ... More

Cellular and gene expression responses involved in the rapid growth inhibition of human cancer cells by RNA interference-mediated depletion of telomerase RNA.

Authors:Li S, Crothers J, Haqq CM, Blackburn EH,

Journal:J Biol Chem

PubMed ID:15831499

Inhibition of the up-regulated telomerase activity in cancer cells has previously been shown to slow cell growth but only after prior telomere shortening. Previously, we have reported that, unexpectedly, a hairpin short interfering RNA specifically targeting human telomerase RNA rapidly inhibits the growth of human cancer cells independently of p53 ... More

IpgB1 is a novel Shigella effector protein involved in bacterial invasion of host cells. Its activity to promote membrane ruffling via Rac1 and Cdc42 activation.

Authors:Ohya K, Handa Y, Ogawa M, Suzuki M, Sasakawa C,

Journal:J Biol Chem

PubMed ID:15849186

Shigella, the causative agent of bacillary dysentery, is capable of inducing the large scale membrane ruffling required for the bacterial invasion of host cells. Shigella secrete a subset of effectors via the type III secretion system (TTSS) into the host cells to induce membrane ruffling. Here, we show that IpgB1 ... More