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Solution Versene
Citations et références (2)
Gibco™

Solution Versene

Versene est une solution EDTA à utiliser comme réactif doux et non enzymatique de dissociation des cellules. La solution Gibco™Afficher plus
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RéférenceQuantité
15040066100 ml
Référence 15040066
Prix (EUR)
25,65
Online Exclusive
28,33
Économisez 2,68 (9%)
Each
Quantité:
100 ml
Prix (EUR)
25,65
Online Exclusive
28,33
Économisez 2,68 (9%)
Each
Versene est une solution EDTA à utiliser comme réactif doux et non enzymatique de dissociation des cellules. La solution Gibco™ Versene (0,48 mM) est formulée comme suit : 0,2 g EDTA(Na4) par litre de solution saline dans un tampon phosphate (PBS).

Utilisation du produit
Usage exclusivement réservé à la recherche. Ne pas utiliser dans les procédures de diagnostic.

Fabrication et système de qualité conformes aux BPFa sur deux sites
La solution Versene Gibco™ est fabriqué sur un site conforme aux BPFa situé à Grand Island, New York, États-Unis. Ce site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485. Pour assurer la continuité de la chaîne d’approvisionnement, nous proposons une solution Gibco™ Versene identique fabriquée sur notre site situé en Écosse (15040-033). Ce site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
ChélateursEDTA
Qualité de fabricationBPFa pour dispositifs médicaux, 21 CFR Partie 820 et ISO 13485
Quantité100 ml
Durée de conservation18 mois
Conditions d’expéditionTempérature ambiante
ClassificationAucun composant d’origine animale
Type de produitRéactif de dissociation de culture cellulaire
StérilitéStérilisation par filtration
Avec additifsEDTA
Unit SizeEach
Contenu et stockage
Conditions de stockage : 2 à 8°C
Conditions d’expédition : température ambiante
Durée de conservation : 18 mois à compter de la date de fabrication

Foire aux questions (FAQ)

Can PSCs transitioned over into Essential 8 Medium on recombinant human laminin-521 (rhLaminin-521) from feeder-dependent cultures, then be transferred onto vitronectin (VTN-N)?

Yes. Following 2 passages on the rhLaminin-521 matrix, Versene or EDTA passaging should be used to subculture PSCs.

What are the main differences to be taken into consideration when culturing cells in Gibco Essential 8 Medium on Gibco Vitronectin (VTN-N) compared to other feeder-free systems?

Here are three major differences to be taken into consideration when culturing cells in Gibco Essential 8 Medium on Gibco Vitronectin (VTN-N) compared to other feeder-free systems:
- Cells should be typically passaged ~24 hours sooner than they would be with other feeder-free media.
- Passaging should take place when cells are at ~85% confluency. If cells are passaged when they are more than 85% confluent, the health of the cells and final cell yield may be compromised.
- Cells must be passaged in EDTA. Collagenase and dispase are not recommended.

Can pluripotent stem cells (PSCs) previously cultured in other media and on other substrates be cultured in Gibco Essential 8 Medium and on Gibco Vitronectin?

Cells cultured in other feeder-free media systems, such as mTeSR Medium with Matrigel Basement Membrane Matrix, or StemPro hESC SFM with Geltrex Matrix, can be successfully cultured in Gibco Essential 8 Medium and VTN-N. In addition, PSCs grown on feeders with KnockOut SR have also been shown to be successfully cultured in Gibco Essential 8 Medium on VTN-N. However, when changing media systems, cells must be passaged either manually, or with EDTA prior to culturing in Gibco Essential 8 Medium on VTN-N.

What is the recommended passaging method to use with Gibco Essential 8 Medium and on Gibco Vitronectin (VTN-N)?

Cells cultured in Gibco Essential 8 Medium and VTN-N need to be passaged with EDTA.

I currently use mTeSR1. How difficult would it be to adapt my cells to the StemFlex Medium system?

This transition is very straightforward. We recommend at least a 2-passage transition into the StemFlex Medium system. Briefly, if you have cryopreserved cells previously cultured in the mTeSR1/Matrigel system, we recommend thawing the cells back into mTeSR1/Matrigel until fully recovered. Upon reaching ~70-85% confluency, passage using Versene solution and seed directly into the StemFlex Medium system.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Solution Versene FAQs

Citations et références (2)

Citations et références
Abstract
Decreased metallation and activity in subsets of mutant superoxide dismutases associated with familial amyotrophic lateral sclerosis.
Authors: Hayward Lawrence J; Rodriguez Jorge A; Kim Ji W; Tiwari Ashutosh; Goto Joy J; Cabelli Diane E; Valentine Joan Selverstone; Brown Robert H Jr;
Journal:J Biol Chem
PubMed ID:11854284
Over 90 different mutations in the gene encoding copper/zinc superoxide dismutase (SOD1) cause approximately 2% of amyotrophic lateral sclerosis (ALS) cases by an unknown mechanism. We engineered 14 different human ALS-related SOD1 mutants and obtained high yields of biologically metallated proteins from an Sf21 insect cell expression system. Both the ... More
Molecular determinants of receptor binding and signaling by the CX3C chemokine fractalkine.
Authors: Mizoue L S; Sullivan S K; King D S; Kledal T N; Schwartz T W; Bacon K B; Handel T M;
Journal:J Biol Chem
PubMed ID:11432858
Fractalkine/CX3CL1 is a membrane-tethered chemokine that functions as a chemoattractant and adhesion protein by interacting with the receptor CX3CR1. To understand the molecular basis for the interaction, an extensive mutagenesis study of fractalkine's chemokine domain was undertaken. The results reveal a cluster of basic residues (Lys-8, Lys-15, Lys-37, Arg-45, and ... More