Citations et références (23)

Gibco™

Pénicilline-streptomycine (5 000 U/ml)

Les antibiotiques pénicilline et streptomycine sont utilisés pour éviter la contamination bactérienne des cultures cellulaires en raison de leur actionAfficher plus

Référence	Quantité
15070063	100 ml

Référence 15070063

Prix (EUR)

25,33

Quantité:

100 ml

Prix (EUR)

25,33

Les antibiotiques pénicilline et streptomycine sont utilisés pour éviter la contamination bactérienne des cultures cellulaires en raison de leur action combinée effective contre les bactéries à Gram positif et à Gram négatif. À l’origine, la pénicilline a été purifiée à partir du champignon Penicillium et agit en interférant directement avec l’activité des parois des cellules bactériennes et indirectement en déclenchant la libération d’enzymes qui continuent à modifier la paroi cellulaire. À l’origine, la streptomycine a été purifiée à partir de Streptomyces griseus. Elle agit en se liant à la sous-unité 30S du ribosome bactérien, ce qui conduit à l’inhibition de la synthèse protéique et à la mort des bactéries sensibles. Cette solution contient 5 000 unités / ml de pénicilline et 5 000 µg / ml de streptomycine.

Nous proposons une large gamme d’antibiotiques et d’antimycotiques dans les formats poudre et liquide. Consultez la liste complète, ou trouvez les produits pour :

• Contrôle de la contamination
• Sélection eucaryotique et bactérienne

Voir les concentrations de travail recommandées pour les antibiotiques de sélection.

En savoir plus sur l’utilisation des antibiotiques et des antimycosiques dans la culture cellulaire et consultez les directives pour la décontamination des cultures.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Concentration100 X

Type de cultureCulture de cellules de mammifères, culture de cellules d’insectes

À utiliser avec (application)Prévention de la contamination par culture cellulaire

Quantité100 ml

Durée de conservation12 mois

Conditions d’expéditionGlace carbonique

FormeLiquide

Type de produitAntibiotique

StérilitéStérilisation par filtration

Unit SizeEach

Contenu et stockage

Conditions de stockage : -5°C à -20°C
Conditions d’expédition : Glace carbonique
Durée de conservation : 12 mois à compter de la date de fabrication

Foire aux questions (FAQ)

My Penicillin-Streptomycin solution is not colorless. Is this normal?

Yes, this is normal and will not affect the potency or application of the product. This solution is typically colorless. However, it can have a pink to yellow color tint. The coloring is a carry-over from the manufacturing process of Streptomycin - the genus that Steptomycin is isolated from (Actinomycetes Streptomyces griseus) is responsible for a wide variety of pigments.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations et références (23)

Citations et références

Abstract

Identification of a novel redox-sensitive gene, Id3, which mediates angiotensin II-induced cell growth.

Authors:Mueller Cornelius; Baudler Stephanie; Welzel Hilke; BÃƒÂ¶hm Michael; Nickenig Georg;

Journal:Circulation

PubMed ID:12021231

BACKGROUND: Reactive oxygen species, such as superoxide (O(2)(-)), are involved in the abnormal growth of various cell types. Angiotensin II (Ang II) is one of the most potent inducers of oxidative stress in the vasculature. The molecular events involved in Ang II-induced proliferation of vascular smooth muscle cells (VSMCs) are ... More

Directed differentiation of pancreatic δ cells from human pluripotent stem cells.

Authors:Chen L,Wang N,Zhang T,Zhang F,Zhang W,Meng H,Chen J,Liao Z,Xu X,Ma Z,Xu T,Liu H

Journal:Nature communications

PubMed ID:39068220

Dysfunction of pancreatic Î´ cells contributes to the etiology of diabetes. Despite their important role, human Î´ cells are scarce, limiting physiological studies and drug discovery targeting Î´ cells. To date, no directed Î´-cell differentiation method has been established. Here, we demonstrate that fibroblast growth factor (FGF) 7 promotes pancreatic ... More

Involvement of c-Src Tyrosine Kinase Upstream of Class I Phosphatidylinositol (PI) 3-Kinases in Salmonella Enteritidis Rck Protein-mediated Invasion.

Authors:Wiedemann A, Rosselin M, Mijouin L, Bottreau E, Velge P,

Journal:J Biol Chem

PubMed ID:22810232

'The Salmonella outer membrane protein Rck mediates a Zipper entry mechanism controlled by tyrosine phosphorylation and class I phosphatidylinositol 3-kinase (PI 3-kinase). However, the underlying mechanism leading to this signaling cascade remains unclear. The present study showed that using Rck-coated beads or Rck-overexpressing Escherichia coli, Rck-mediated actin polymerization and invasion ... More

A differential role for the mitogen-activated protein kinases in lipopolysaccharide signaling: the MEK/ERK pathway is not essential for nitric oxide and interleukin 1beta production.

Authors: Watters Jyoti J; Sommer Julie A; Pfeiffer Zachary A; Prabhu Usha; Guerra Alma N; Bertics Paul J;

Journal:J Biol Chem

PubMed ID:11786532

'Endotoxin (lipopolysaccharide, LPS) is a component of the outer membrane of Gram-negative bacteria and promotes the activation of macrophages and microglia. Although these cells are highly LPS-responsive, they serve unique tissue-specific functions and exhibit different LPS sensitivities. Accordingly, it was of interest to evaluate whether these biological differences reside in ... More

Electrophoretic profiling of both RNA and protein from a single 250-pL sample.

Authors: Zabzdyr Jennifer L; Lillard Sheri J;

Journal:Anal Chem

PubMed ID:11985318

'A novel approach is described that uses capillary electrophoresis (CE) to electrophoretically sample and separate both protein and RNA from a single injected plug of cell lysate. A 250-pL sample of lysate from Chinese hamster ovary cells (9.6 x 10(7) cells/mL) was hydrodynamically injected into a capillary containing a Tris-based ... More

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