Antibiotique-antimycotique (100X)
Gibco™

Antibiotique-antimycotique (100X)

L’antibiotique-antimycosique Gibco™ est utilisé pour empêcher la contamination bactérienne et fongique. Cette solution contient 10 000 unités / ml deAfficher plus
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RéférenceQuantité
15240062100 mL
1524009620 mL
Référence 15240062
Prix (EUR)
92,65
Offre exceptionnelle en ligne
109,00
Économisez 16,35 (15%)
Each
Quantité:
100 mL
Prix (EUR)
92,65
Offre exceptionnelle en ligne
109,00
Économisez 16,35 (15%)
Each
L’antibiotique-antimycosique Gibco™ est utilisé pour empêcher la contamination bactérienne et fongique. Cette solution contient 10 000 unités / ml de pénicilline, 10 000 µg / ml de streptomycine et 25 µg / ml d’amphotéricine Gibco B. Les antibiotiques pénicilline et streptomycine empêchent la contamination bactérienne des cultures cellulaires en raison de leur action combinée efficace contre les bactéries à Gram-positif et Gram-négatif. L’amphotéricine B empêche la contamination fongique des cultures cellulaires en raison de son inhibition de champignons multicellulaires et de levures.

À l’origine, la pénicilline a été purifiée à partir du champignon Penicillium et agit en interférant directement avec l’activité des parois des cellules bactériennes et indirectement en déclenchant la libération d’enzymes qui continuent à modifier la paroi cellulaire. À l’origine, la streptomycine a été purifiée à partir de Streptomyces griseus. Elle agit en se liant à la sous-unité 30S du ribosome bactérien, ce qui conduit à l’inhibition de la synthèse protéique et à la mort des bactéries sensibles. L’amphotéricine B est un agent antifongique qui inhibe la croissance des champignons et des levures en augmentant la perméabilité de la membrane du plasma fongique.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Concentration100 X
Type de cultureCulture de cellules de mammifères
Quantité100 mL
Durée de conservation12 mois
Conditions d’expéditionGlace carbonique
FormeLiquide
Type de produitAntibiotique-antimycosique
StérilitéStérilisation par filtration
Sterilization MethodStérilisation par filtration
Unit SizeEach
Contenu et stockage
Conditions de stockage : -5°C à -20°C
Conditions d’expédition : Glace carbonique
Durée de conservation : 12 mois à compter de la date de fabrication

Foire aux questions (FAQ)

Can I use pen/strep or other antibiotics for episomal reprogramming of cells?

The use of antibiotics is not recommended during episomal reprogramming, especially Fungizone antimycotic. If antibiotics must be used during the reprogramming process, pen/strep could be used. For established iPSCs (after reprogramming is complete), either antibiotic/antimycotic or pen/strep should be fine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use antibiotics such as Pen-Strep or Antibiotic-Antimycotic (100X) when culturing Sf9 insect cells?

Yes, however, we do not recommend adding antibiotics to the medium at the time of thawing. Thaw cells into medium without antibiotic and allow the cells to recover from the thawing process. After that, you can add antibiotics by diluting into the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the solvent used in Antibiotic-Antimycotic (100X)?

The components are in a 0.85% saline solution.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the components of Antibiotic-Antimycotic (100X)?

This solution contains 10,000 units/mL of penicillin, 10,000 µg/mL of streptomycin, and 25 µg/mL of Gibco Amphotericin B.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the recommended storage condition for Antibiotic-Antimycotic (100X)?

The recommended condition is storage at -5 to -20 degrees C and when stored as recommended, the shelf life is 12 months from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations et références (13)

Citations et références
Abstract
Common variation at 6q16 within HACE1 and LIN28B influences susceptibility to neuroblastoma.
Authors:Diskin SJ, Capasso M, Schnepp RW, Cole KA, Attiyeh EF, Hou C, Diamond M, Carpenter EL, Winter C, Lee H, Jagannathan J, Latorre V, Iolascon A, Hakonarson H, Devoto M, Maris JM,
Journal:Nat Genet
PubMed ID:22941191
Neuroblastoma is a cancer of the sympathetic nervous system that accounts for approximately 10% of all pediatric oncology deaths. Here, we report a genome-wide association study of 2,817 neuroblastoma cases and 7,473 controls. We identified two new associations at 6q16, the first within HACE1 (rs4336470; combined P=2.7×10(-11); odds ratio 1.26, ... More
Patient-derived glioblastoma organoids as real-time avatars for assessing responses to clinical CAR-T cell therapy
Authors:Meghan Logun, Xin Wang, Yusha Sun, Stephen J Bagley, Nannan Li, Arati Desai, Daniel Y Zhang, MacLean P Nasrallah, Emily Ling-Lin Pai, Bike Su Oner, Gabriela Plesa, Donald Siegel, Zev A Binder, Guo-Li Ming, Hongjun Song, Donald M O'Rourke
Journal:Cell Stem Cell
PubMed ID:39657679
Patient-derived tumor organoids have been leveraged for disease modeling and preclinical studies but rarely applied in real time to aid with interpretation of patient treatment responses in clinics. We recently demonstrated early efficacy signals in a first-in-human, phase 1 study of dual-targeting chimeric antigen receptor (CAR)-T cells (EGFR-IL13Rα2 CAR-T cells) ... More
Non-viral-mediated gene transfer of OX40 ligand for tumor immunotherapy.
Authors:Rakitina OA,Kuzmich AI,Bezborodova OA,Kondratieva SA,Pleshkan VV,Zinovyeva MV,Didych DA,Sass AV,Snezhkov EV,Kostina MB,Koksharov MO,Alekseenko IV
Journal:Frontiers in immunology
PubMed ID:39007148
BACKGROUND: Immune checkpoint blockade (ICB) is rapidly becoming a standard of care in the treatment of many cancer types. However, the subset of patients who respond to this type of therapy is limited. Another way to promote antitumoral immunity is the use of immunostimulatory molecules, such as cytokines or T ... More
Protocol to generate human liver spheroids to study liver fibrosis induced by metabolic stress.
Authors:Kim HY,Lee W,Liu X,Jang H,Sakane S,Carvalho-Gontijo Weber R,Diggle K,Kerk SA,Metallo CM,Kisseleva T,Brenner DA
Journal:STAR protocols
PubMed ID:38833372
Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models. Here, we present a protocol to generate 3D human liver spheroids that contain all the liver cell types and mimic “livers in a dish.” We describe strategies to induce ... More
Single-Cell Transcriptome Atlas of Murine Endothelial Cells.
Authors:Kalucka J, de Rooij LPMH, Goveia J, Rohlenova K, Dumas SJ, Meta E, Conchinha NV, Taverna F, Teuwen LA, Veys K, García-Caballero M, Khan S, Geldhof V, Sokol L, Chen R, Treps L, Borri M, de Zeeuw P, Dubois C, Karakach TK, Falkenberg KD, Parys M, Yin X, Vinckier S, Du Y, Fenton RA, Schoonjans L, Dewerchin M, Eelen G, Thienpont B, Lin L, Bolund L, Li X, Luo Y, Carmeliet P
Journal:Cell
PubMed ID:32059779
'The heterogeneity of endothelial cells (ECs) across tissues remains incompletely inventoried. We constructed an atlas of >32,000 single-EC transcriptomes from 11 mouse tissues and identified 78 EC subclusters, including Aqp7' ... More