Mélange d’antibiotiques Pénicilline-Streptomycine-Néomycine (PSN)

Citations et références (9)

Gibco™

Mélange d’antibiotiques Pénicilline-Streptomycine-Néomycine (PSN)

Les antibiotiques pénicilline, streptomycine et néomycine sont utilisés pour éviter la contamination bactérienne des cultures cellulaires en raison de leurAfficher plus

Référence	Quantité
15640055	100 ml

Référence 15640055

Prix (EUR)

72,00

Quantité:

100 ml

Prix (EUR)

72,00

Les antibiotiques pénicilline, streptomycine et néomycine sont utilisés pour éviter la contamination bactérienne des cultures cellulaires en raison de leur action combinée effective contre les bactéries à Gram positif et à Gram négatif. À l’origine, la pénicilline a été purifiée à partir du champignon Penicillium et agit en interférant directement avec l’activité des parois des cellules bactériennes et indirectement en déclenchant la libération d’enzymes qui continuent à modifier la paroi cellulaire. À l’origine, la streptomycine a été purifiée à partir de Streptomyces griseus et la néomycine à partir de Streptomyces fradiae. La streptomycine et la néomycine agissent en se liant à la sous-unité 30S du ribosome bactérien, ce qui conduit à l’inhibition de la synthèse protéique et à la mort des bactéries sensibles. Nous proposons une variété d’antibiotiques et d’antimycotiques pour les applications de culture cellulaire.

Cette solution contient 5 mg de pénicilline, 5 mg de streptomycine et 10 mg de néomycine par ml dans une solution saline à 0,85 %.

Utilisation du produit
Usage exclusivement réservé à la recherche : Ne pas utiliser à des fins diagnostiques ni thérapeutiques humaines ou animales.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Concentration100 X

Type de cultureCulture de cellules de mammifères

À utiliser avec (application)Prévention de la contamination par culture cellulaire

Quantité100 ml

Conditions d’expéditionGlace carbonique

FormeLiquide

Type de produitAntibiotique

StérilitéStérilisation par filtration

Unit SizeEach

Contenu et stockage

Conserver au congélateur (-5°C à -20°C).

Foire aux questions (FAQ)

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations et références (9)

Citations et références

Abstract

Characterization of the 46-kDa intermediates of matrix metalloproteinase 3 (stromelysin 1) obtained by site-directed mutation of phenylalanine 83.

Authors: Benbow U; ButticÃ¨ G; Nagase H; Kurkinen M;

Journal:J Biol Chem

PubMed ID:8631880

The precursor of matrix metalloproteinase 3 (MMP-3/ stromelysin 1) is activated in vitro by proteinases or mercurial compounds by stepwise processes which include the initial formation of short-lived intermediates and the subsequent intermolecular cleavage of the His82-Phe83 bond to generate the fully activated mature MMP-3 (Nagase, H., Enghild, J. J., ... More

Pyridoxine induces glutathione synthesis via PKM2-mediated Nrf2 transactivation and confers neuroprotection.

Authors:Wei Y, Lu M, Mei M, Wang H, Han Z, Chen M, Yao H, Song N, Ding X, Ding J, Xiao M, Hu G

Journal:Nat Commun

PubMed ID:32071304

'Oxidative stress is a major pathogenic mechanism in Parkinson''s disease (PD). As an important cellular antioxidant, glutathione (GSH) balances the production and incorporation of free radicals to protect neurons from oxidative damage. GSH level is decreased in the brains of PD patients. Hence, clarifying the molecular mechanism of GSH deficiency ... More

Isorhynchophylline ameliorates cognitive impairment via modulating amyloid pathology, tau hyperphosphorylation and neuroinflammation: Studies in a transgenic mouse model of Alzheimer's disease.

Authors:Li HQ, Ip SP, Yuan QJ, Zheng GQ, Tsim KKW, Dong TTX, Lin G, Han Y, Liu Y, Xian YF, Lin ZX

Journal:Brain Behav Immun

PubMed ID:31476414

'Isorhynchophylline (IRN) has been demonstrated to have distinct anti-Alzheimer''s disease (AD) activity in several animal models of AD. In this study, we aimed at evaluating the preventive effect of IRN on the cognitive deficits and amyloid pathology in TgCRND8 mice. Male TgCRND8 mice were administered with IRN (20 or 40?mg/kg) ... More

miR-210 promotes progression of endometrial carcinoma by regulating the expression of NFIX.

Authors:Yang L, Yang Z, Yao R, Li Y, Liu Z, Chen X, Zhang G

Journal:Int J Clin Exp Pathol

PubMed ID:31949601

'microRNA-210 (miR-210) plays an important role in human disease, but its function in endometrial cancer (EC) is still unclear. Similarly, the nuclear factor I/X (NFIX) plays an important role in various biological functions of cells, but its function in EC is not yet known. In this study, we detected the ... More

Regulation of proapoptotic proteins Bak1 and p53 by miR-125b in an experimental model of Alzheimer's disease: Protective role of 17ß-estradiol.

Authors:Micheli F, Palermo R, Talora C, Ferretti E, Vacca A, Napolitano M

Journal:Neurosci Lett

PubMed ID:27235580

Alzheimer's disease has become one of the most impacting disorders since world population is rapidly aging. MicroRNA-125b plays a crucial role in many cellular processes and pathologies, but, to date, its role in Alzheimer's disease is controversial. In this study, we demonstrated, for the first time, that the down regulation ... More

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