Gentamicine (10 mg/ml)
Gibco™

Gentamicine (10 mg/ml)

Le sulfate de gentamicine est un antibiotique hydrosoluble issu à l’origine de la purification du champignon Micromonospora purpurea. La gentamicineAfficher plus
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RéférenceQuantité
1571006410 ml
Référence 15710064
Prix (EUR)
16,65
キャンペーン価格
17,87
Économisez 1,22 (7%)
Each
Quantité:
10 ml
Prix (EUR)
16,65
キャンペーン価格
17,87
Économisez 1,22 (7%)
Each
Le sulfate de gentamicine est un antibiotique hydrosoluble issu à l’origine de la purification du champignon Micromonospora purpurea. La gentamicine agit en se liant à la sous-unité 30S du ribosome bactérien, ce qui conduit à l’inhibition de la protéosynthèse et à la mort des bactéries sensibles. La gentamicine Gibco™ est efficace contre un vaste éventail de bactéries à Gram positif et certaines bactéries à Gram négatif ; elle est utilisée en prévention de la contamination bactérienne des cultures cellulaires. Les plages de concentration de travail recommandées s’échelonnent entre 0,5 et 50 µg/ml. Nous proposons un grand choix d’antibiotiques et d’antimycotiques pour les applications de culture cellulaire.

Utilisation du produit
uniquement à des fins de recherche : Ne pas utiliser à des fins diagnostiques ni thérapeutiques humaines ou animales.

Fabrication conforme aux BPFa sur deux sites
La gentamycine Gibco™ est fabriqué sur un site conforme aux BPFa situé à Grand Island, New York (États-Unis). Ce site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485. Pour assurer la continuité de la chaîne d’approvisionnement, nous proposons un produit comparable à la gentamicine Gibco™ fabriqué sur notre site situé en Écosse (15710-049). Ce site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications
Concentration10 mg/ml
Type de cultureCulture de cellules de mammifères
À utiliser avec (application)Prévention de la contamination par culture cellulaire
Quantité10 ml
Durée de conservation24 mois
Conditions d’expéditionTempérature ambiante
FormeLiquide
Type de produitAntibiotique
StérilitéStérilisation par filtration
Unit SizeEach
Contenu et stockage
Conditions de stockage : 15 à 30°C
Conditions d’expédition : température ambiante
Durée de conservation : 24 mois à compter de la date de fabrication

Foire aux questions (FAQ)

If Gentamicin (10 mg/mL) is accidentally stored at 2-8 degrees C, would it affect the stability of the antibiotic?

No, storing Gentamicin solution for several days at 2-8 degrees C will not have any negative impact on its performance or stability. However, as Gentamicin solution has been shown to be stable at room temperature, the recommended storage temperature is ~25 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations et références (6)

Citations et références
Abstract
Novel Cav2.1 splice variants isolated from Purkinje cells do not generate P-type Ca2+ current.
Authors: Tsunemi Taiji; Saegusa Hironao; Ishikawa Kinya; Nagayama Shin; Murakoshi Takayuki; Mizusawa Hidehiro; Tanabe Tsutomu;
Journal:J Biol Chem
PubMed ID:11756409
'The alpha(1)2.1 (alpha(1A)) subunits of P-type and Q-type Ca(2+) channels are encoded by a single gene, Cacna1a. Although these channels differ in the inactivation kinetics and sensitivity to omega-agatoxin IVA, the mechanism underlying these differences remains to be clarified. Alternative splicings of the Cacna1a transcript have been postulated to contribute ... More
Cell-autonomous impact of polysialic acid-producing enzyme ST8SIA2 on developmental migration and distribution of cortical interneurons.
Authors:Schuster UE, Rossdam C, Röckle I, Schiff M, Hildebrandt H
Journal:J Neurochem
PubMed ID:31608978
'In humans, variations in the polysialic acid-producing enzyme ST8SIA2 and disturbances in the cortical inhibitory system are associated with neurodevelopmental psychiatric disorders such as schizophrenia and autism. In mice, the ST8SIA2-dependent formation of polysialic acid during embryonic development is crucial for the establishment of interneuron populations of the medial prefrontal ... More
Identification of Novel Protein Targets of Dimethyl Fumarate Modification in Neurons and Astrocytes Reveals Actions Independent of Nrf2 Stabilization.
Authors:Piroli GG, Manuel AM, Patel T, Walla MD, Shi L, Lanci SA, Wang J, Galloway A, Ortinski PI, Smith DS, Frizzell N
Journal:Mol Cell Proteomics
PubMed ID:30587509
'The fumarate ester dimethyl fumarate (DMF) has been introduced recently as a treatment for relapsing remitting multiple sclerosis (RRMS), a chronic inflammatory condition that results in neuronal demyelination and axonal loss. DMF is known to act by depleting intracellular glutathione and modifying thiols on Keap1 protein, resulting in the stabilization ... More
Vesicular Stomatitis Virus Transcription Is Inhibited by TRIM69 in the Interferon-Induced Antiviral State.
Authors:Kueck T, Bloyet LM, Cassella E, Zang T, Schmidt F, Brusic V, Tekes G, Pornillos O, Whelan SPJ, Bieniasz PD
Journal:J Virol
PubMed ID:31578292
'Interferons (IFNs) induce the expression of interferon-stimulated genes (ISGs), many of which are responsible for the cellular antiviral state in which the replication of numerous viruses is blocked. How the majority of individual ISGs inhibit the replication of particular viruses is unknown. We conducted a loss-of-function screen to identify genes ... More
Use of Precision-Cut Lung Slices as an
Authors:Rosales Gerpe MC, van Vloten JP, Santry LA, de Jong J, Mould RC, Pelin A, Bell JC, Bridle BW, Wootton SK
Journal:Mol Ther Methods Clin Dev
PubMed ID:30112421
Organotypic slice cultures recapitulate many features of an intact organ, including cellular architecture, microenvironment, and polarity, making them an ideal tool for the