Gentamicine (50 mg/ml)
Gentamicine (50 mg/ml)
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Citations et références (13)
Gibco™

Gentamicine (50 mg/ml)

Le sulfate de gentamycine est un antibiotique hydrosoluble obtenu à l’origine par purification du champignon Micromonospora purpurea.Il agit en seAfficher plus
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RéférenceQuantité
1575006010 ml
1575007810 x 10 ml
Référence 15750060
Prix (EUR)
72,65
Online Exclusive
83,25
Économisez 10,60 (13%)
Each
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Quantité:
10 ml
Prix (EUR)
72,65
Online Exclusive
83,25
Économisez 10,60 (13%)
Each
Ajouter au panier
Le sulfate de gentamycine est un antibiotique hydrosoluble obtenu à l’origine par purification du champignon Micromonospora purpurea.Il agit en se liant à la sous-unité 30S du ribosome bactérien, conduisant à l’inhibition de la protéosynthèse et à la mort des bactéries sensibles.La gentamycine Gibco™ est efficace contre de très nombreuses bactéries à Gram positif et à Gram négatif : elle est utilisée pour empêcher la contamination des cultures cellulaires par des bactéries.Les plages de concentration de travail recommandées s’échelonnent entre 0,5 et 50 μg/ml.

Nous proposons une vaste gamme d’antibiotiques et d’antimycotiques dans les formats poudre et liquide.

En savoir plus sur l’utilisation d’antibiotiques et d’antimycotiques dans la culture cellulaire et consulter les directives pour la décontamination des cultures.

Fabrication conforme aux BPFa sur deux sites
La gentamicine Gibco™ est fabriqué dans une installation conforme aux BPFa, située à Grand Island, dans l’État de New York.Le site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485.Pour assurer la continuité de la chaîne d’approvisionnement, nous proposons un produit comparable à la gentamicine Gibco™ fabriqué sur notre site situé en Écosse (15750-037).Ce site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Concentration50 mg/mL
Température de stockageConditions de stockage : 15°C à 30°C
Conditions d’expédition : température ambiante
Durée de conservation : 24 mois à compter de la date de fabrication
Conditions d’expéditionTempérature ambiante
Stérilisée par filtrationYes
Application validéePrévention de la contamination par culture cellulaire
Forme physiqueLiquide
Quantité10 ml
TypeGentamicine
Unit SizeEach

Foire aux questions (FAQ)

Once Gentamicin (50 mg/mL; Cat. No. 15750060) is added to the cell culture medium, can this resulting medium, including gentamicin, be frozen and thawed before using it in cell culture without affecting the performance of the antibiotic?

Yes, gentamicin can be frozen in a prepared medium. However, we don't have any stability data on this process with media not made here in our facility. So, you would have to determine the effectiveness of the product upon thawing empirically.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations et références (13)

Citations et références
Abstract
A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.
Authors:Bonanini F,Singh M,Yang H,Kurek D,Harms AC,Mardinoglu A,Hankemeier T
Journal:Experimental cell research
PubMed ID:38499143
Biosynthetic studies of A2E, a major fluorophore of retinal pigment epithelial lipofuscin.
Authors: Ben-Shabat Shimon; Parish Craig A; Vollmer Heidi R; Itagaki Yasuhiro; Fishkin Nathan; Nakanishi Koji; Sparrow Janet R;
Journal:J Biol Chem
PubMed ID:11756445
'We have examined questions related to the biosynthesis of A2E, a fluorophore that accumulates in retinal pigment epithelial cells with aging and in some retinal disorders. The use of in vitro preparations revealed that detectable levels of A2-PE, the A2E precursor, are formed within photoreceptor outer segments following light-induced release ... More
Growth-dependent Regulation of Mammalian Pyrimidine Biosynthesis by the Protein Kinase A and MAPK Signaling Cascades.
Authors: Sigoillot Frederic D; Evans David R; Guy Hedeel I;
Journal:J Biol Chem
PubMed ID:11872754
'The carbamoyl phosphate synthetase domain of the multifunctional protein CAD catalyzes the initial, rate-limiting step in mammalian de novo pyrimidine biosynthesis. In addition to allosteric regulation by the inhibitor UTP and the activator PRPP, the carbamoyl phosphate synthetase activity is controlled by mitogen-activated protein kinase (MAPK)- and protein kinase A ... More
The potent anti-HIV protein cyanovirin-N contains two novel carbohydrate binding sites that selectively bind to Man(8) D1D3 and Man(9) with nanomolar affinity: implications for binding to the HIV envelope protein gp120.
Authors: Bewley C A; Otero-Quintero S
Journal:J Am Chem Soc
PubMed ID:11457139
'Cyanovirin-N (CVN) is a monomeric 11 kDa cyanobacterial protein that potently inactivates diverse strains of human immunodeficiency virus (HIV) at the level of cell fusion by virtue of high affinity interactions with the surface envelope glycoprotein gp120. Several lines of evidence have suggested that CVN-gp120 interactions are in part mediated ... More
Isolation of carbohydrate-specific CD4(+) T cell clones from mice after stimulation by two model glycoconjugate vaccines.
Authors:Avci FY, Li X, Tsuji M, Kasper DL,
Journal:Nat Protoc
PubMed ID:23196974
Here we describe how to isolate carbohydrate-specific T cell clones (for which we propose the designation 'Tcarbs') after stimulation by two glycoconjugate vaccines. We describe how to prepare, purify and characterize two model glycoconjugate vaccines that can be used to generate Tcarbs. These glycoconjugate vaccines (GBSIII-OVA and GBSIII-TT) are synthesized ... More
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