Gentamicine (50 mg/ml)
Gentamicine (50 mg/ml)
Citations et références (5)
Gibco™

Gentamicine (50 mg/ml)

Le sulfate de gentamycine est un antibiotique hydrosoluble obtenu à l’origine par purification du champignon Micromonospora purpurea.Il agit en seAfficher plus
Have Questions?
Modifier l'affichagebuttonViewtableView
RéférenceQuantité
1575007810 x 10 ml
1575006010 ml
Référence 15750078
Prix (EUR)
584,65
Online Exclusive
666,00
Économisez 81,35 (12%)
Each
Ajouter au panier
Quantité:
10 x 10 ml
Prix (EUR)
584,65
Online Exclusive
666,00
Économisez 81,35 (12%)
Each
Ajouter au panier
Le sulfate de gentamycine est un antibiotique hydrosoluble obtenu à l’origine par purification du champignon Micromonospora purpurea.Il agit en se liant à la sous-unité 30S du ribosome bactérien, conduisant à l’inhibition de la protéosynthèse et à la mort des bactéries sensibles.La gentamycine Gibco™ est efficace contre de très nombreuses bactéries à Gram positif et à Gram négatif : elle est utilisée pour empêcher la contamination des cultures cellulaires par des bactéries.Les plages de concentration de travail recommandées s’échelonnent entre 0,5 et 50 μg/ml.

Nous proposons une vaste gamme d’antibiotiques et d’antimycotiques dans les formats poudre et liquide.

En savoir plus sur l’utilisation d’antibiotiques et d’antimycotiques dans la culture cellulaire et consulter les directives pour la décontamination des cultures.

Fabrication conforme aux BPFa sur deux sites
La gentamicine Gibco™ est fabriqué dans une installation conforme aux BPFa, située à Grand Island, dans l’État de New York.Le site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485.Pour assurer la continuité de la chaîne d’approvisionnement, nous proposons un produit comparable à la gentamicine Gibco™ fabriqué sur notre site situé en Écosse (15750-037).Ce site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Concentration50 mg/mL
Température de stockageConditions de stockage : 15°C à 30°C
Conditions d’expédition : température ambiante
Durée de conservation : 24 mois à compter de la date de fabrication
Conditions d’expéditionTempérature ambiante
Stérilisée par filtrationYes
Application validéePrévention de la contamination par culture cellulaire
Forme physiqueLiquide
Quantité10 x 10 ml
TypeGentamicine
Unit SizeEach

Foire aux questions (FAQ)

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations et références (5)

Citations et références
Abstract
Involvement of c-Src Tyrosine Kinase Upstream of Class I Phosphatidylinositol (PI) 3-Kinases in Salmonella Enteritidis Rck Protein-mediated Invasion.
Authors:Wiedemann A, Rosselin M, Mijouin L, Bottreau E, Velge P,
Journal:J Biol Chem
PubMed ID:22810232
'The Salmonella outer membrane protein Rck mediates a Zipper entry mechanism controlled by tyrosine phosphorylation and class I phosphatidylinositol 3-kinase (PI 3-kinase). However, the underlying mechanism leading to this signaling cascade remains unclear. The present study showed that using Rck-coated beads or Rck-overexpressing Escherichia coli, Rck-mediated actin polymerization and invasion ... More
Isolation of carbohydrate-specific CD4(+) T cell clones from mice after stimulation by two model glycoconjugate vaccines.
Authors:Avci FY, Li X, Tsuji M, Kasper DL,
Journal:Nat Protoc
PubMed ID:23196974
Here we describe how to isolate carbohydrate-specific T cell clones (for which we propose the designation 'Tcarbs') after stimulation by two glycoconjugate vaccines. We describe how to prepare, purify and characterize two model glycoconjugate vaccines that can be used to generate Tcarbs. These glycoconjugate vaccines (GBSIII-OVA and GBSIII-TT) are synthesized ... More
A three-dimensional co-culture system to investigate macrophage-dependent tumor cell invasion.
Authors:Dwyer AR, Ellies LG, Holme AL, Pixley FJ
Journal:J Biol Methods
PubMed ID:31453214
'Macrophages infiltrate cancers and promote progression to invasion and metastasis. To directly examine tumor-associated macrophages (TAMs) and tumor cells interacting and co-migrating in a three-dimensional (3D) environment, we have developed a co-culture model that uses a PyVmT mouse mammary tumor-derived cell line and mouse bone marrow-derived macrophages (BMM). The Py8119 ... More
Characterizing the Antimicrobial Function of a Dairy-Originated Probiotic,
Authors:Nair DVT, Kollanoor Johny A
Journal:Front Microbiol
PubMed ID:30050507
'Antimicrobial potential of a dairy-origin probiotic bacteria,'
Palbociclib treatment alters nucleotide biosynthesis and glutamine dependency in A549 cells.
Authors:Conroy LR, Lorkiewicz P, He L, Yin X, Zhang X, Rai SN, Clem BF
Journal:Cancer Cell Int
PubMed ID:32624705
Aberrant activity of cell cycle proteins is one of the key somatic events in non-small cell lung cancer (NSCLC) pathogenesis. In most NSCLC cases, the retinoblastoma protein tumor suppressor (RB) becomes inactivated via constitutive phosphorylation by cyclin dependent kinase (CDK) 4/6, leading to uncontrolled cell proliferation. Palbociclib, a small molecule ... More