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Sérum de veau fœtal, certifié, États-Unis
Citations et références (10)
Gibco™

Sérum de veau fœtal, certifié, États-Unis

Les sérums de veau fœtal Gibco offrent une excellente valeur pour la culture cellulaire de base, la recherche spécialisée etAfficher plus
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RéférenceQuantitéFormat
16000044500 mLFlacon
A316040150 mLOne Shot
A316040210 x 50 mLOne Shot
Our improved Premium Plus Fetal Bovine Serum (FBS) features enhanced specifications with unchanged stability, manufacturing, storage, and transport processes.
Référence 16000044
Les sérums de veau fœtal Gibco offrent une excellente valeur pour la culture cellulaire de base, la recherche spécialisée et les dosages spécifiques. Ils bénéficient de la confiance des chercheurs grâce à un soutien primé et d’une qualité constante, répondant à vos besoins en matière de recherche et de budget

Sérums Gibco : Qualité fiable pour une performance constante

Catégorie du sérum : Premium (Performance Plus)
• Utilisés dans une vaste gamme de types de cellules, particulièrement dans les lignées cellulaires sensibles
• Nos sérums les plus caractérisés offrent les plus bas niveaux d’endotoxine et d’hémoglobine
• Niveau d’endotoxine : ≤ 5 UE/ml
• Niveau d’hémoglobine : ≤ 15 mg/dl
• Origine : États-Unis

Les sérum Gibco sont
• Certifiés ISO 13485 et traités dans des installations homologuées par la FDA
• Marqués pour le diagnostic in vitro (conformes aux niveaux de contrôles de stérilité USP les plus élevés)
• Filtrés trois fois à 0,1 µm
• Le flacon Gibco est plus facile à utiliser dans la hotte, réduisant le risque de contamination et vous aidant à réaliser une culture cellulaire plus régulière
• L’autocollant détachable sur l’étiquette fournit une référence pratique permettant d’enregistrer le numéro de lot et la date de péremption dans le cahier de laboratoire
À des fins de recherche ou de commercialisation avancée Non destiné aux diagnostics ou à l’administration directe à des humains ou des animaux.
Spécifications
Concentration en endotoxines≤5 UE/mL
Concentration d’hémoglobine≤ 15 mg/dl
Pureté ou qualitéCertifiés
Conditions d’expéditionCongelé
EspècesBovine
AgeFœtal
Pays d’origineÉtats-Unis
FormeLiquide
FormatFlacon
Type de produitSérum de veau fœtal
Quantité500 mL
Serum TreatmentStandard (filtration stérile)
StérilitéStérile
Sterilization MethodTriple filtration, 0,1 μm
Unit SizeEach
Contenu et stockage
Conditions de stockage : ≤-10°C
Conditions d’expédition : Congelé

Foire aux questions (FAQ)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How much of the total protein measured in Fetal Bovine Serum (heat inactivated or otherwise) is attributed to albumin?

Our CoAs only capture the total protein content not specifically the amount attributed to albumin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How much Fetal Bovine Serum (FBS) do I need to add to my medium?

FBS is added to culture medium at a concentration of 2-10% to provide attachment factors, nutrients, and hormones for mammalian cells, as well as to be a buffer against disruptions like pH changes and endotoxins. FBS has significant amounts of embryonic growth promoting factors like hormones, carrier proteins, and macromolecular proteins. It also has low levels of antibodies and other growth-inhibiting components. For most basal medium, 10% FBS is used. Some applications require up to 20% supplementation, so researchers need to determine what is optimal for their specific application and cell line.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Sérum de veau fœtal, certifié, États-Unis FAQs

Citations et références (10)

Citations et références
Abstract
Fibroblast growth factor receptor-1 signaling induces osteopontin expression and vascular smooth muscle cell-dependent adventitial fibroblast migration in vitro.
Authors: Li Guohong; Oparil Suzanne; Kelpke Stacey S; Chen Yiu-Fai; Thompson John A;
Journal:Circulation
PubMed ID:12176960
'BACKGROUND: Increased expression of osteopontin (OPN), fibroblast growth factors (FGFs), and their type-1 receptor (FGFR-1) is associated with neointima formation and atherosclerosis. This study tested the hypothesis that ligand activation of FGFR-1 stimulates OPN expression in rat aortic smooth muscle cells (RASMCs), explored the signaling pathway involved, and assessed the ... More
Ultraviolet-induced junD activation and apoptosis in myeloblastic leukemia ML-1 cells.
Authors:Li T, Dai W, Lu L.
Journal:J Biol Chem
PubMed ID:12082101
'The exposure of mammalian cells to UV irradiation induces the expression of immediate early genes such as c-jun and c-fos and activates the transcription factors AP-1 and NF-kappaB. JunD is one of the three members of the Jun family and shares some functional characteristics with c-Jun. In the present study, ... More
Dystrophin deficiency markedly increases enterovirus-induced cardiomyopathy: a genetic predisposition to viral heart disease.
Authors: Xiong Dingding; Lee Gil-Hwan; Badorff Cornel; Dorner Andrea; Lee Sang; Wolf Paul; Knowlton Kirk U;
Journal:Nat Med
PubMed ID:12118246
Both enteroviral infection of the heart and mutations in the dystrophin gene can cause cardiomyopathy. Little is known, however, about the interaction between genetic and acquired forms of cardiomyopathy. We previously demonstrated that the enteroviral protease 2A cleaves dystrophin; therefore, we hypothesized that dystrophin deficiency would predispose to enterovirus-induced cardiomyopathy. ... More
Ascorbic-acid transporter Slc23a1 is essential for vitamin C transport into the brain and for perinatal survival.
Authors: Sotiriou Sotiria; Gispert Suzana; Cheng Jun; Wang Yaohui; Chen Amy; Hoogstraten-Miller Shelley; Miller Georgina F; Kwon Oran; Levine Mark; Guttentag Susan H; Nussbaum Robert L;
Journal:Nat Med
PubMed ID:11984580
The only proven requirement for ascorbic acid (vitamin C) is in preventing scurvy, presumably because it is a cofactor for hydroxylases required for post-translational modifications that stabilize collagen. We have created mice deficient in the mouse ortholog (solute carrier family 23 member 1 or Slc23a1) of a rat ascorbic-acid transporter, ... More
Cell docking and on-chip monitoring of cellular reactions with a controlled concentration gradient on a microfluidic device.
Authors: Yang Mengsu; Li Cheuk-Wing; Yang Jun;
Journal:Anal Chem
PubMed ID:12199565
We have developed a microfluidic device for on-chip monitoring of cellular reactions. The device consists of two primary analytical functions: control of cell transport and immobilization, and dilution of an analyte solution to generate a concentration gradient. In this device, a dam structure in parallel to the fluid flow was ... More
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