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Sérum de veau fœtal, qualifié, inactivé par la chaleur, États-Unis
Sérum de veau fœtal, qualifié, inactivé par la chaleur, États-Unis
Citations et références (1)
Gibco™

Sérum de veau fœtal, qualifié, inactivé par la chaleur, États-Unis

Les sérums de veau fœtal Gibco offrent une excellente valeur pour la culture cellulaire de base, la recherche spécialisée etAfficher plus
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RéférenceQuantitéFormat
16140071500 mLFlacon
A384010150 mLOne Shot
A384010210 x 50 mLOne Shot
Our improved Premium Fetal Bovine Serum (FBS) features enhanced specifications with unchanged stability, manufacturing, storage, and transport processes.
Référence 16140071
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Les sérums de veau fœtal Gibco offrent une excellente valeur pour la culture cellulaire de base, la recherche spécialisée et les dosages spécifiques. Ils bénéficient de la confiance des chercheurs grâce à un soutien primé et d’une qualité constante, répondant à vos besoins en matière de recherche et de budget

Catégorie du sérum : Valeur plus élevée (performance)
Origine : États-Unis.
Niveau d’endotoxine : ≤ 10 UE/ml
Niveau d’hémoglobine : ≤ 25 mg/dl.
For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Spécifications
Concentration en endotoxines≤ 10 EU/mL
Concentration d’hémoglobine≤ 25 mg/dl
Pureté ou qualitéQualifié
Conditions d’expéditionCongelé
EspècesBovine
AgeFœtal
Pays d’origineÉtats-Unis
FormeLiquide
FormatFlacon
Type de produitSérum de veau fœtal
Quantité500 mL
Serum TreatmentInactivé par la chaleur
Unit SizeEach
Contenu et stockage
Conditions de stockage : ≤-10°C
Conditions d’expédition : Congelé

Foire aux questions (FAQ)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I minimize lot-to-lot variability in serum?

You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco iMatch Sera Lot Matching Tool.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and refreeze serum?

Yes, after the overnight thaw, you can aliquot the serum into smaller, single-use aliquots and refreeze it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Sérum de veau fœtal, qualifié, inactivé par la chaleur, États-Unis FAQs

Citations et références (1)

Citations et références
Abstract
A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.
Authors:Bonanini F,Singh M,Yang H,Kurek D,Harms AC,Mardinoglu A,Hankemeier T
Journal:Experimental cell research
PubMed ID:38499143