L-glutamine (200 mM)
Gibco™

L-glutamine (200 mM)

La L-Glutamine est un amino-acide nécessaire à la culture cellulaire. La L-Glutamine participe à la formation des nucléotides purine etAfficher plus
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RéférenceQuantité
25030081100 ml
2503014920 ml
Référence 25030081
Prix (EUR)
34,01
Each
Quantité:
100 ml
Customize this product
Prix (EUR)
34,01
Each
La L-Glutamine est un amino-acide nécessaire à la culture cellulaire. La L-Glutamine participe à la formation des nucléotides purine et pyrimidine, des sucres aminés, du glutathion, du L-glutamate, et autres amino-acides, ainsi qu’à la synthèse de protéines et à la production de glucose. Contrairement à la plupart des autres amino-acides, la L-glutamine n’est pas stable en solution. La rapidité avec laquelle la détérioration se produit dépend du temps, de la température et du pH. La L-glutamine Gibco™ est une solution de réserve de 200 mM prête à l’emploi qui agit comme un supplément de culture cellulaire. La concentration optimale dépend du type de cellule et du milieu utilisé pour la culture cellulaire, mais est généralement comprise entre 2– 6 mM.

Nous proposons également un complément GlutaMAX™ Gibco™ en guise d’alternative stable à la L-glutamine.

Système de fabrication et de Qualité BPFa sur deux sites
La L-glutamine Gibco™ est fabriquée dans un établissement conforme aux BPFa, situé à Paisley, en Écosse, au Royaume-Uni. Ce site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485. Pour la continuité de la chaîne d’approvisionnement, nous proposons un produit identique à la L-glutamine Gibco™ fabriqué dans notre installation de Grand Island (25030-081). Ce site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485.
Spécifications
Nom chimique ou matériauGlutamine
Concentration or Composition (by Analyte or Components)100 X
Concept écologiqueEmballage durable
Forme physiqueLiquide
Gamme de produitsGibco
Température de stockageConditions de stockage : -5°C à -20°C À l’abri de la lumière
Conditions d’expédition : Glace carbonique
Durée de conservation : 24 mois à compter de la date de fabrication
Durée de conservation24 mois
Conditions d’expéditionGlace carbonique
Quantité100 ml
pH6 à 8
Unit SizeEach

Foire aux questions (FAQ)

Why did my 200 mM L-Glutamine precipitate out of solution when I thawed it?

When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why does L-Glutamine (200 mM) (Cat. No. 25050149, 25030081, 25030164) solution have “Product of Brazil” on the label?

L-Glutamine (200 mM) (Cat. No. 25050149, 25030081, 25030164) solution is made in the USA from a raw material glutamine powder manufactured in Brazil. The country of manufacture of a component that has been subjected to manufacturing or processing operations in more than one country is the last country in which the component has been “substantially transformed”. A “substantial transformation” occurs in a country when a product emerges from a manufacturing process with a name, character, or use different from that of the original material(s) subjected to that process.

Diluting, purifying, sorting, testing, packing, repacking, or affixing labels to a product in a country does not constitute a “substantial transformation” in that country, hence the label must bear the country of the original raw material origin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My order of L-Glutamine (Cat. No. 25030081) arrived frozen. Is it stable?

If L-Glutamine is completely or partially frozen, it is still stable. It becomes unstable when stored for extended periods of time completely thawed, especially above 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is your recommended method for thawing L-Glutamine before adding to cell culture media?

We recommend thawing at 2-8 degrees C and then warming at 37 degrees C until the material goes into solution. Mix it as it warms up to spend minimal time at elevated temperatures before you aliquot it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why did my 200 mM L-glutamine precipitate when I thawed it?

When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37 degrees C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations et références (10)

Citations et références
Abstract
Structural and energetic characteristics of the heparin-binding site in antithrombotic protein C.
Authors:Friedrich U, Blom AM, Dahlbäck B, Villoutreix BO,
Journal:J Biol Chem
PubMed ID:11316800
'Human activated protein C (APC) is a key component of a natural anticoagulant system that regulates blood coagulation. In vivo, the catalytic activity of APC is regulated by two serpins, alpha1-antitrypsin and the protein C inhibitor (PCI), the inhibition by the latter being stimulated by heparin. We have identified a ... More
FRET measurements of intracellular cAMP concentrations and cAMP analog permeability in intact cells.
Authors:Börner S, Schwede F, Schlipp A, Berisha F, Calebiro D, Lohse MJ, Nikolaev VO,
Journal:Nat Protoc
PubMed ID:21412271
'Real-time measurements of second messengers in living cells, such as cAMP, are usually performed by ratiometric fluorescence resonance energy transfer (FRET) imaging. However, correct calibration of FRET ratios, accurate calculations of absolute cAMP levels and actual permeabilities of different cAMP analogs have been challenging. Here we present a protocol that ... More
A critical role for choline kinase-alpha in the aggressiveness of bladder carcinomas.
Authors:Hernando E, Sarmentero-Estrada J, Koppie T, Belda-Iniesta C, Ramírez de Molina V, Cejas P, Ozu C, Le C, Sánchez JJ, González-Barón M, Koutcher J, Cordón-Cardó C, Bochner BH, Lacal JC, Ramírez de Molina A,
Journal:Oncogene
PubMed ID:19448670
'Bladder cancer is one of the most common causes of death in industrialized countries. New tumor markers and therapeutic approaches are still needed to improve the management of bladder cancer patients. Choline kinase-alpha (ChoKalpha) is a metabolic enzyme that has a role in cell proliferation and transformation. Inhibitors of ChoKalpha ... More
Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O.
Authors:Walev I, Bhakdi SC, Hofmann F, Djonder N, Valeva A, Aktories K, Bhakdi S,
Journal:Proc Natl Acad Sci U S A
PubMed ID:11248053
'The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10(5)-10(6) molecules were estimated to be entrapped per cell. Repair of toxin lesions depended on Ca(2+)-calmodulin and on ... More
Fabrication of synthetic polymer coatings and their use in feeder-free culture of human embryonic stem cells.
Authors:Nandivada H, Villa-Diaz LG, O'Shea KS, Smith GD, Krebsbach PH, Lahann J,
Journal:Nat Protoc
PubMed ID:21720316
The culture of human embryonic stem (hES) cells in defined and xenogeneic-free conditions will contribute substantially to future biotechnological and medical applications. To achieve this goal, we developed the first fully defined synthetic polymer coating poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH) that sustains long-term growth of hES cells in different culture media. ... More