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Sérum de veau fœtal, dialysé, origine américaine
Citations et références (2)
Gibco™

Sérum de veau fœtal, dialysé, origine américaine

Les sérums de veau fœtal Gibco™ offrent une excellente valeur pour la culture cellulaire de base, la recherche spécialisée etAfficher plus
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RéférenceQuantitéFormat
26400044500 mLFlacon
A338200150 mLOne Shot
Référence 26400044
Prix (EUR)
961,65
Online Exclusive
1 080,00
Économisez 118,35 (11%)
Each
Quantité:
500 mL
Format:
Flacon
Prix (EUR)
961,65
Online Exclusive
1 080,00
Économisez 118,35 (11%)
Each
Les sérums de veau fœtal Gibco™ offrent une excellente valeur pour la culture cellulaire de base, la recherche spécialisée et les dosages spécifiques. Ils bénéficient de la confiance des chercheurs grâce à un soutien primé et d’une qualité constante, répondant à vos besoins en matière de recherche et de budget

Catégorie du sérum : Spécifique
Origine : États-Unis.
Niveau d’endotoxine : ≤ 50 UE/ml (spécifications contrôlées et enregistrées, généralement ≤ 10 UE/ml)
Niveau d’hémoglobine : ≤ 25 mg/dl
À des fins de recherche ou de commercialisation avancée Non destiné aux diagnostics ou à l’administration directe à des humains ou des animaux.
Spécifications
Conditions d’expéditionCongelé
EspècesBovine
AgeFœtal
Pays d’origineÉtats-Unis
FormeLiquide
FormatFlacon
Type de produitSérum de veau fœtal
Quantité500 mL
Serum TreatmentDialysé
Unit SizeEach
Contenu et stockage
Conditions de stockage : ≤-10°C
Conditions d’expédition : Congelé

Foire aux questions (FAQ)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are proteins removed from Fetal Bovine Serum, dialyzed (Cat. No. 26400044, A3382001)?

The raw serum is processed through a dialysis system that has a 10 kDa cutoff. Therefore, many proteins, peptides, as well as some hormones, glucose, amino acids, and other exogenous materials are removed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I minimize lot-to-lot variability in serum?

You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco iMatch Sera Lot Matching Tool.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Sérum de veau fœtal, dialysé, origine américaine FAQs

Citations et références (2)

Citations et références
Abstract
Folate deficiency inhibits the proliferation of primary human CD8+ T lymphocytes in vitro.
Authors:Courtemanche C, Elson-Schwab I, Mashiyama ST, Kerry N, Ames BN,
Journal:J Immunol
PubMed ID:15322179
'Folate is required for one-carbon transfer reactions and the formation of purines and pyrimidines for DNA and RNA synthesis. Deficiency of folate can lead to many clinical abnormalities, including macrocytic anemia, cardiovascular diseases, birth defects, and carcinogenesis. The nucleotide imbalance due to folate deficiency causes cell cycle arrest in the ... More
Characterization of the 46-kDa intermediates of matrix metalloproteinase 3 (stromelysin 1) obtained by site-directed mutation of phenylalanine 83.
Authors: Benbow U; Butticè G; Nagase H; Kurkinen M;
Journal:J Biol Chem
PubMed ID:8631880
The precursor of matrix metalloproteinase 3 (MMP-3/ stromelysin 1) is activated in vitro by proteinases or mercurial compounds by stepwise processes which include the initial formation of short-lived intermediates and the subsequent intermolecular cleavage of the His82-Phe83 bond to generate the fully activated mature MMP-3 (Nagase, H., Enghild, J. J., ... More