Échelle de protéines à plage basse et non colorée PageRuler™
Échelle de protéines à plage basse et non colorée PageRuler™
Thermo Scientific™

Échelle de protéines à plage basse et non colorée PageRuler™

L’échelle de protéines à gamme basse et non colorée Thermo Scientific PageRuler est un mélange de huit protéines et peptidesAfficher plus
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RéférenceQuantité
266322 x 250 μl
26632X48 x 250 μL
Référence 26632
Prix (EUR)
102,00
Each
Ajouter au panier
Quantité:
2 x 250 μl
Grand volume ou format personnalisé
Prix (EUR)
102,00
Each
Ajouter au panier
L’échelle de protéines à gamme basse et non colorée Thermo Scientific PageRuler est un mélange de huit protéines et peptides purifiés (3,4 à 100 kDa) à utiliser comme étalons de dimensionnement lors de l’électrophorèse sur gel de peptides et de protéines de petite taille. L’échelle de protéines est prête à l’emploi pour le chargement direct sur les gels ; nul besoin de la chauffer, de la réduire ou d’ajouter un tampon d’échantillon avant de l’utiliser.

Découvrez et comparez tous les autres étalons et échelles de protéines ›

Caractéristiques du produit
• Bandes de référence : la bande de 25 kDa est plus intense que les autres pour une orientation facile
• Marquée : les protéines de l’échelle (sauf les peptides de 5 et 3,4 kDa) contiennent une séquence Strep-tag™ II intégrale et peuvent être détectées lors des transferts Western à l’aide de conjugués Strep-Tactin™

Applications
• Dimensionnement précis des protéines sur gels de polyacrylamide SDS et transferts Western

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications
À utiliser avec (application)Tricine gels
Compatibilité du gelGels Bolt™ Bis-Tris Plus, gels Novex™ Tricine, gels Novex™ Tris-Glycine, gels NuPAGE™ Bis-Tris, gels NuPAGE™ Tris-Acétate, gels SDS-PAGE
Poids moléculaire100, 30, 25, 20, 15, 10, 5, 3,4 kDa
Gamme de produitsPageRuler
Type de produitÉchelle de protéines
Quantité2 x 250 μl
Prêt à chargerOui
Applications recommandéesGels Tricine
Conditions d’expéditionHomologué pour des expéditions sur de la glace carbonique ou mouillée
Type de colorationNon coloré
Type de systèmeSDS-PAGE
Number of Markers8
Plage de dimensions3,4 à 100 kDa
Unit SizeEach
Contenu et stockage
Contenu : deux flacons de 250 μl chacun

Tampon de stockage : 62,5 mM de Tris-H3PO4 (pH 7,5 à 25°C), 1 mM d’EDTA, 2 % de SDS, 100 mM de DTT, 1 mM de NaN3, 0,01 % de bleu de bromophénol et 33 % de glycérol

Stockage : Dès réception, conserver à -20°C

Foire aux questions (FAQ)

The upper bands of the ladder are missing. What could be the reason?

The upper bands of the ladder may be degraded by proteases. Ladder, gel, buffer, pipettes, pipette tips, or equipment can be contaminated by proteases during usage. A general recommendation would be to avoid working with proteases in the same room. We would recommend preparing fresh solutions, cleaning the equipment, and using clean pipettes and tips. If the ladder itself is contaminated, please use a new tube of the ladder.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Some additional bands or smears were observed on the gel when using a PageRuler unstained ladder. What may have caused this?

Additional bands can appear due to dithiothreitol (DTT) oxidation in the storage buffer. Please add newly prepared DTT solution to the final concentration of 100 mM and boil for 5 min at 95 degrees C. This should solve the issue. Addition of DTT is NOT recommended for prestained protein ladders, since too high a concentration of reducing agents can cause protein destaining.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do the proteins in Thermo Scientific protein ladders have a His-Tag or would otherwise react with an anti-His-Tag antibody?

No, proteins in Thermo Scientific protein ladders are not His tagged. However, non-specific interaction between the ladder proteins and primary or secondary antibodies is possible and some His-Tag detection systems, such as Thermo Scientific 6xHis Protein Tag Stain Reagent Kit, show non-specific interaction. The protein ladder bands are more readily detected when using high antibody concentrations. The non-specific cross-reactivity is difficult to predict, it often has a different pattern dependent on the antibodies used in each individual experiment. The most general way to handle this problem would be to use lower concentrations of antibodies and to use lower amount of protein ladders. It may also be useful to leave one empty well between the ladder and the sample to overcome a possible leakage of the signal to the nearby sample lane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can Thermo Scientific protein ladders be detected by Strep-Tactin conjugates?

PageRuler Unstained protein ladders can be detected directly on Western blots by using Strep-Tactin conjugates or an antibody against the Strep-tag II sequence. All PageRuler and Spectra ladder proteins contain an integral Strep-tag II sequence, however the prestained ladders cannot be detected by Strep-Tactin conjugates.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Why is non-specific binding detected after Western blot?

Protein ladder bands can sometimes be detected with chemiluminescent techniques due to non-specific interactions of ladder proteins with either primary or secondary antibodies (or with both). The ladder bands are only rarely detected by chromogenic substrates. The extremely high sensitivity of the chemiluminescent assays is needed to see the bands, so the actual degree of cross-reactivity is low. The non-specific cross-reactivity is difficult to predict, it often has a different pattern depending on the antibodies used. If antibodies recognize a linear epitope, the cross-reactivity may be due to sequence homology. If antibodies react with a denaturation-resistant conformational epitope it could be impossible to identify the exact reason for detected cross-reactivity. The most general way to handle this problem would be to use lower concentrations of antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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