POROS™ CaptureSelect™ FibXL Affinity Matrix

POROS CaptureSelect FibXL Affinity Matrix has specifically been designed for the purification of human fibrinogen from recombinant or plasma source.

Features of this affinity matrix include:

• Fibrinogen purification from human plasma and recombinant sources
• Efficient purification of fibrinogen with high yields and retained activity
• Excellent scalability
• Non-animal derived
• High flow rates

POROS CaptureSelect FibXL Affinity Matrix is an affinity matrix that binds to fibrinogen with high selectivity and yield, enabling a robust, fast, and efficient purification process with excellent purity obtained in one step.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

• Matrix: cross-linked poly(styrene-divinylbenzene)
• Average particle size: 50 μm
• Ligand: POROS CaptureSelect FibXL affinity ligand
• Ligand coupling method: epoxide
• Binding capacity: approximately 15 g/L resin (determined with plasma derived fibrinogen)
• Elution conditions: acidic elution with 0.1 M glycine pH 3.0; neutral pH elution with 20 mM Tris, 1.0 M MgCl₂ and 40% (v/v) propyleneglycol
• Mechanical resistance: 100 bar (1450 psi, 10 MPa)
• Formulation buffer: 20 % (v/v) ethanol