POROS™ CaptureSelect™ IgM-XL Affinity Matrix

POROS CaptureSelect IgM-XL Affinity Matrix is intended for the purification of human IgM, with no binding to other immunoglobulins.

Features of this affinity matrix include:

• IgM purification from plasma and serum
• Purification of monoclonal IgM from cell cultures
• Efficient purification of IgM with high yields and retained activity
• Excellent scalability
• Non-animal derived
• High flow rates
• Cross-reactivity towards mouse and rat IgM

POROS CaptureSelect IgM-XL Affinity Matrix binds to the Fc part of IgM. The high selectivity and yields obtained using this affinity matrix enable a robust, fast, and efficient purification process with excellent purity obtained in one step.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (animal origin-free).

Main characteristics

• Matrix: cross-linked poly(styrene-divinylbenzene)
• Average particle size: 50 μm
• Ligand: POROS CaptureSelect IgM-XL affinity ligand
• Ligand coupling method: epoxide coupling
• Binding capacity: >5 g/L resin (determined with polyclonal human IgM from serum)
• Elution conditions: acidic elution with 0.1 M glycine, pH 3.0
• Mechanical resistance: 100 bar (1450 psi, 10 MPa)
• Formulation buffer: 20% (v/v) ethanol