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Citations et références (38623)
Applied Biosystems™
7500 Real-Time PCR System, laptop
The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This systemAfficher plus
Référence 4351104
Prix (EUR)Demander un devis
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The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This system is a sophisticated platform for users who require extended capabilities and maximum versatility. The 3rd generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.
• Powerful, five-color platform is calibrated for the broadest range of dyes available: FAM™⁄SYBR® Green I, VIC®⁄JOE™, NED™⁄ TAMRA™⁄ Cy3™, ROX™⁄Texas Red™, and Cy5™ dyes
• Specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets
• Advanced multi-componenting algorithm minimizes spectral crosstalk – superior for multiplexing
• User-friendly software includes plate set-up wizards, multi-plate data viewing capabilities, and advanced analysis tools to make data processing simple and straightforward
NEW - 7500 Software v2.x
Now the easy-to-use StepOne software is available for both the 7500 and 7500 Fast systems with the 7500 Software v2.x upgrade. The 7500 Software v2.x incorporates your favorite StepOne Software features, such as a variety of plate setup wizards, standard curve dilution and master mix recipe calculators, QC flags, data filters, and email notification when a run is finished. The 7500 Software v2.x also includes an enhanced Gene Expression Study package and has a variety of new melting curve protocol options, including multiple peak detection, step and hold temperature control, and customizable ramp rates.
The NEW Gene Expression Study package accommodates large studies better than any other instrument software package:
• Import an unlimited number of Comparative CT (relative quantitation) files to one study
• Group samples and view data both by technical replicate group and biological replicate group
• Use any gene(s) as an endogenous control, including averaging multiple controls together
• Enter known efficiency values to be factored into the RQ results
21 CFR Part 11 Module available
The SDS v1.4 21CFRp11 Module is a powerful tool for assisting with 21CFRp11 compliance, while still offering the flexibility of user-customizable configuration settings.
• Individual user log-ins can be added for up to four user groups, each group with designated permission settings
• User-customizable permission settings include fourteen system activities, e-signature authority designation, and additional security settings to give you maximum control over your compliance efforts
• Audit trails can be enabled or disabled depending on your traceability needs
• A selection of e-signatures is available to ease e-signatures into your workflow
Supports Many Applications
Applications include gene expression analysis, pathogen quantitation, SNP genotyping, isothermal and +⁄– assays utilizing internal positive controls. To facilitate many of these applications, Applied Biosystems® provides preformulated, ready-to-use, quality-tested, TaqMan® assays for use with the 7500 system. Now you can reduce your assay optimization efforts.
Upgrade to High-Speed Thermal Cycling
An optional upgrade to the 7500 Fast System is available. This 7500 Fast System uses our master mix formulations and enables you to shorten your real-time PCR runs to as little as 30 minutes.
For Research Use Only. Not for use in diagnostics procedures.
• Powerful, five-color platform is calibrated for the broadest range of dyes available: FAM™⁄SYBR® Green I, VIC®⁄JOE™, NED™⁄ TAMRA™⁄ Cy3™, ROX™⁄Texas Red™, and Cy5™ dyes
• Specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets
• Advanced multi-componenting algorithm minimizes spectral crosstalk – superior for multiplexing
• User-friendly software includes plate set-up wizards, multi-plate data viewing capabilities, and advanced analysis tools to make data processing simple and straightforward
NEW - 7500 Software v2.x
Now the easy-to-use StepOne software is available for both the 7500 and 7500 Fast systems with the 7500 Software v2.x upgrade. The 7500 Software v2.x incorporates your favorite StepOne Software features, such as a variety of plate setup wizards, standard curve dilution and master mix recipe calculators, QC flags, data filters, and email notification when a run is finished. The 7500 Software v2.x also includes an enhanced Gene Expression Study package and has a variety of new melting curve protocol options, including multiple peak detection, step and hold temperature control, and customizable ramp rates.
The NEW Gene Expression Study package accommodates large studies better than any other instrument software package:
• Import an unlimited number of Comparative CT (relative quantitation) files to one study
• Group samples and view data both by technical replicate group and biological replicate group
• Use any gene(s) as an endogenous control, including averaging multiple controls together
• Enter known efficiency values to be factored into the RQ results
21 CFR Part 11 Module available
The SDS v1.4 21CFRp11 Module is a powerful tool for assisting with 21CFRp11 compliance, while still offering the flexibility of user-customizable configuration settings.
• Individual user log-ins can be added for up to four user groups, each group with designated permission settings
• User-customizable permission settings include fourteen system activities, e-signature authority designation, and additional security settings to give you maximum control over your compliance efforts
• Audit trails can be enabled or disabled depending on your traceability needs
• A selection of e-signatures is available to ease e-signatures into your workflow
Supports Many Applications
Applications include gene expression analysis, pathogen quantitation, SNP genotyping, isothermal and +⁄– assays utilizing internal positive controls. To facilitate many of these applications, Applied Biosystems® provides preformulated, ready-to-use, quality-tested, TaqMan® assays for use with the 7500 system. Now you can reduce your assay optimization efforts.
Upgrade to High-Speed Thermal Cycling
An optional upgrade to the 7500 Fast System is available. This 7500 Fast System uses our master mix formulations and enables you to shorten your real-time PCR runs to as little as 30 minutes.
For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.
Spécifications
Colorant étalonnéSYBR™ Green I, ROX™, NED™, TAMRA™, JOE™, Texas Red™, Cy5™, VIC™, FAM™, Cy3™
DimensionsWidth 34 cm (13.39 in.), Depth 45 cm (17.72 in.), Height 49 cm (19.29 in.)
Plage dynamique9 Logs
Alimentation électrique950 W
Ordinateur externeNotebook: Intel Core 2 Duo T5500 (1.66 GHz), 8X DVD, 80 GB HD, 1.0 G DDR2-533 MHz SDRAM
À utiliser avec (équipement)Système 7500
FormatPlaque 96 puits
Compatibilité à haut débitMultiplexage
Vitesse d’échauffement max. (bloc thermique)2.5°C
Éléments optiquesCCD Camera, 5 Excitation Filters, Tungsten-halogen Lamp, 5 Emission Filters
Colorant de référence passiveROX (tube distinct), sans ROX, ROX (prémélangé)
Pic de montée en température du bloc2.5°C⁄s
Précision99.7% Confidence (5000 & 10000 Copies)
Quantité1 system
Vitesse de réactionÉtalon
Plage de volume de réaction20–100 μL
Montée en température des échantillons± 1.1°C⁄s
Sensibilité1 Copy of RNase P gene (Human Genomic DNA)
Compatibilité logiciellePrimer Express, 21 CFR Part 11 Module (optional), 7500 System Software (RQ included)
Plage de températures (métrique)4 à 100°C
Précision thermique0.25°C (35°C to 95°C after 3 min.)
Système de cyclage thermiqueSystème basé sur Peltier
Uniformité thermique0.50°C (after 30 s)
Cadence4 to 5-96-well Plates per 8-hr day
Tension120 V
Poids34 kg (75 lb.)
Unit SizeEach
Foire aux questions (FAQ)
Why are there no RQ values for all the samples in my study from the 7500 or 7500 Fast Real-Time PCR System software?
Why do I not see any CT or RQ values from my ddCT experiment in the software for the 7500 or 7500 Fast Real-Time PCR System?
What can I do when I get the error message "Time too short" on the 7500 or 7500 Fast Real-Time PCR System?
What can I do if I get an error message about "Block Up Switch" on the 7500 or 7500 Fast Real-Time PCR System?
On the 7500 and 7500 Fast Real-Time PCR Systems, what can I do if I get an error message about "CCD acquisition failure"?
Citations et références (38623)
Citations et références
Abstract
Role of APE1 in differentiated neuroblastoma SH-SY5Y cells in response to oxidative stress: Use of APE1 small molecule inhibitors to delineate APE1 functions
Journal:DNA REPAIR
PubMed ID:
Oxidative DNA damage has been implicated in a number of central nervous system pathologies. The base excision repair (BER) pathway is one of the most important cellular protection mechanisms that respond to oxidative DNA damage. Human apurinic (apyrimidinic) endonuclease/redox effector factor (APE1/Ref1 or APE1) is an essential enzyme in the
Characterization of matrix metalloproteinase-2 and matrix metalloproteinase-9 and their inhibitors in equine granulosa cells in vivo and in vitro
Journal:JOURNAL OF ANIMAL SCIENCE
PubMed ID:
Matrix metalloproteinases (MMP) and tissue inhibitors of MMP (TIMP) regulate tissue remodeling events necessary for ovulation. Thus, changes in MMP and TIMP expression and protein enzyme activity were examined in vivo and in vitro during follicular development and atresia in the horse. Equine granulosa cells and follicular fluid from medium
The time of prenatal immune challenge determines the specificity of inflammation-mediated brain and behavioral pathology.
Journal:J Neurosci
PubMed ID:16672647
Disturbance to early brain development is implicated in several neuropsychiatric disorders including autism, schizophrenia, and mental retardation. Epidemiological studies have indicated that the risk of developing these disorders is enhanced by prenatal maternal infection, presumably as a result of neurodevelopmental defects triggered by cytokine-related inflammatory events. Here, we demonstrate that
Distinct roles for IFN regulatory factor (IRF)-3 and IRF-7 in the activation of antitumor properties of human macrophages.
Journal:Cancer Res
PubMed ID:17079482
When properly activated, macrophages can be tumoricidal, thus making them attractive additions to standard cancer therapies. To this end, tolerance and activity of human autologous IFN-gamma-activated macrophages, produced in large scale for clinical use (MAK cells), have been assessed in pilot trials in cancer patients. In the present study, we
Trps1 plays a pivotal role downstream of Gdf5 signaling in promoting chondrogenesis and apoptosis of ATDC5 cells.
Journal:Genes Cells
PubMed ID:18363966
Tricho-rhino-phalangeal syndrome (TRPS) is an autosomal dominant skeletal disorder caused by mutations of TRPS1. Based on the similar expression patterns of Trps1 and Gdf5, we hypothesized a possible functional interaction between these two molecules. Using a chondrogenic cell line (ATDC5), we investigated the association of Gdf5-mediated signaling pathways with