Thermo Scientific™

Colonnes HPLC CurePac™ Bio 550 SEC MDi™

Name: Colonnes HPLC CurePac™ Bio 550 SEC MDi™
SKU: 43903-154631
Price: 2655.64 EUR

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Référence	Longueur (métrique)	Diamètre (métrique)	Format de colonne	Débit
43903-154631	150 mm	4,6 mm	Colonne analytique	0,6 ml/min
43903-152131	150 mm	2,1 mm	Colonne analytique	125 μl/min
43903-157831	150 mm	7,8 mm	Colonne analytique	1,0 ml/min.
43903-032131	30 mm	2,1 mm	Pré-colonne	125 μl/min
43903-034631	30 mm	4,6 mm	Pré-colonne	0,6 ml/min
43903-037831	30 mm	7,8 mm	Pré-colonne	1,0 ml/min.

6 options

Référence 43903-154631

Prix (EUR)

2 655,64

Each

Longueur (métrique):

150 mm

Diamètre (métrique):

4,6 mm

Format de colonne:

Colonne analytique

Débit:

0,6 ml/min

Prix (EUR)

2 655,64

Each

Experience the future of Size-Exclusion Chromatography (SEC) with SurePac™ Bio 550 SEC MDi™ analytical columns and guards exclusively designed to deliver robust and reproducible performance at high resolution and throughput to accelerate your therapeutics analysis process. The column's groundbreaking MDi™ technology integrates our advanced 3 μm monodisperse silica particle platform with bioinert hardware to ensure superior reliability for best sample recovery. SurePac™ Bio 550 SEC MDi™ column is ideal for AAV gene therapeutics analysis and offers excellent selectivity due to the dedicated smaller particle (3 μm) size combined with wide-pore (550Å) for separating monomeric AAVs from aggregates based on their size differences.

Les colonnes et colonnes de protection SurePac Bio 550 SEC MDi sont basées sur des particules de silice de haute pureté, sphériques, poreuses (550 Å) et monodispersées de 3 μm, modifiées de manière covalente avec une couche hydrophile diol exclusive. Cette conception unique permet d’obtenir des distances de diffusion plus courtes et un meilleur transfert de masse reproductible, ainsi que des pics plus fins. La taille constante de ces particules facilitera un contrôle précis de la synthèse des milieux et du conditionnement des colonnes, ce qui améliore considérablement la reproductibilité entre colonnes et entre lots. Les colonnes sont logées dans un matériel en acier inoxydable hydrophile recouvert de bioinert afin d’éliminer les interactions secondaires, ce qui garantit des performances optimales pendant le processus d’injection initial.

Idéal pour la caractérisation des thérapies géniques AAV et l’évaluation du contrôle de qualité grâce à la sélectivité des petites particules dédiées (3 μm) combinée à une taille de pores large (550 Å).
Reproductibilité supérieure de colonne à colonne et de lot à lot, ce qui supprime le problème de variabilité des colonnes dans les tests de stabilité et d’AQ/CQ
Pics haute efficacité avec excellente récupération
Une résolution et un débit plus élevés pour un délai d’exécution rapide sans nuire à la performance
Matériel bioinerte pour éliminer l’adsorption des analytes

Spécifications

Format de colonneColonne analytique

Débit0,6 ml/min

Pression max.3 500 psi (241 bars)

Matériau de remplissageRésine de silice monodispersée

Quantité1 de chaque

Phase stationnaireExclusif

Température20°C to 60°C

Diamètre (métrique)4,6 mm

Longueur (métrique)150 mm

Granulométrie3 μm

PhaseExclusion stérique

TechniqueSEC

pH2 à 8

Unit SizeEach

Foire aux questions (FAQ)

What types of column formats and flow conditions are available for SurePac Bio 550 SEC MDi columns, and how do I choose the right one?

The SurePac Bio 550 SEC MDi column is offered in multiple formats: e.g., 150 mm length × 4.6 mm ID (flow ~0.6 mL/min), 150 mm × 2.1 mm ID (flow ~125 µL/min), and guard formats (30 mm length) in various diameters.
Considerations for choosing the right format of SurePac Bio 550 SEC MDi column:
• Sample volume: larger diameter (4.6 and 7.8 mm) is compatible with larger sample volume, when you need to inject a lot of samples to achieve the required sensitivity
• System compatibility: Ensure your LC pump and detector make-up flow can support chosen flow rates without pressure issues.
• Solvent usage and dead volume: Smaller ID columns save solvent and reduce dilution—a factor in high-sensitivity labs.

What sample types or applications are well-suited for SurePac Bio 550 SEC MDi columns. Are there samples for which these columns are not the best choice?

SurePac Bio 550 SEC MDi columns are well-suited for:
• AAV (adeno-associated virus) monomer/aggregate separations in gene-therapy analytical labs.
• Biopharmaceuticals where high resolution of large biomolecules/aggregates is required.
• Large mRNA and DNA molecules
• Quality control or stability-testing workflows where reproducibility is critical.
The SurePac Bio 550 SEC MDi column may not be ideal if:
• SEC of small proteins because pores are too large
• You do not require the high resolution/throughput that 3 µm monodisperse columns provide. In such cases, a simpler, lower-cost SEC column might suffice.

What makes the MDi technology in SurePac Bio 550 SEC MDi columns different from conventional SEC column packings?

The MDi (“Monodisperse & inert hardware”) technology of the SurePac Bio 550 SEC MDi column incorporates monodisperse 3 µm silica particles (uniform size) plus bio-inert column hardware (to minimize undesired sample interactions).

Compared to standard polydisperse packings (e.g., 5 µm or larger silica particles, or less inert hardware), this leads to:
• Enhanced reproducibility (column-to-column, lot-to-lot)
• Sharper peaks (due to small particles)
• Faster separations at equal resolution compared to columns packed with traditional 5 µm particles (because smaller particles deliver higher plate counts with shorter columns)

What analytical challenges do SurePac Bio 550 SEC MDi columns help solve?

SurePac Bio 550 SEC MDi columns are size-exclusion HPLC columns designed for bio-therapeutic separations (notably AAV gene-therapy workflows) using a 3 µm monodisperse silica packing with ~550 Å pore size and bio-inert hardware. These columns help address challenges such as accurate separation of monomeric versus aggregated viral particles (or other large-biomolecule aggregates) with high resolution and reproducibility - a workflow where many conventional SEC columns may struggle due to broad peaks or secondary interactions.