Applied Biosystems™

Megaplex™ PreAmp Primers, Human Pool A v2.1

Pour une utilisation lorsque la sensibilité du dosage est capitale, les amorces PreAmp Megaplex™ améliorent significativement la capacité de détecterAfficher plus

Référence	Quantité
4399233	50 réactions

Référence 4399233

Prix (EUR)

990,00

Quantité:

50 réactions

Prix (EUR)

990,00

Pour une utilisation lorsque la sensibilité du dosage est capitale, les amorces PreAmp Megaplex™ améliorent significativement la capacité de détecter les microARN à faible expression, permettant la génération d’un profil d’expression complet en utilisant beaucoup moins d’échantillons, dès 1 ng d’ARN total d’entrée.Avec une teneur correspondant aux amorces Megaplex™ RT et aux amorces Megaplex™ PreAmp, le groupe humain A v2.1 est conçu pour être utilisé avec les amorces Megaplex™ RT, groupe humain A v2.1.Suite à une transcription inverse à l’aide d’amorces RT Megaplex™, les amorces PreAmp Megaplex™ ainsi que le Master Mix Preamp TaqMan® amplifient uniformément tous les microARN avant la quantification par PCR en temps réel à l’aide de cartes microARN TaqMan® Array.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Nbre de réactions50 réactions

AmorceAmorces PreAmp

Gamme de produitsMegaplex, TaqMan

Type de produitAmorce de préamplification

Quantité50 réactions

Conditions d’expéditionTempérature ambiante

EspècesHumaine

Suffisant pour50 réactions

GC-Rich PCR PerformanceFaible

Méthode PCRqPCR

Unit SizeEach

Contenu et stockage

Stockage à -20°C.

Foire aux questions (FAQ)

I want to purchase TaqMan Array Human MicroRNA A Cards v2.0 (Cat. No. 4398965). What other reagents do I need to purchase in addition to these cards?

In order to perform reverse transcription on these cards, you will need the following:

TaqMan MicroRNA Reverse Transcription Kit (Cat. No. 4366596)
Megaplex RT Primers pools (for human Type A cards Cat. No. 4399966, for Type B cards Cat. No. 4444281)

You can proceed with or without a preamplification step which depends on the total RNA amount. Total RNA amount of 1‐1000 ng supports reverse transcription reaction with preamplification, whereas 350‐1000 ng of total RNA supports a reaction without preamplification. In case you are performing preamplification you would also need the TaqMan PreAmp Master Mix (Cat. No. 4391128) and the Megaplex PreAmp Primers, Human Pool A v2.1 (Cat. No. 4399233). You will also need the Master Mix for the qPCR reaction. It is not included in the card kit. The recommended Master Mix is: TaqMan Fast Advanced Master Mix – run mode Fast (Cat. No. 4444556) For further information, please see the following link.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

Why is the Ct value for the no-template control (NTC) <35 for some TaqMan microRNA Assays?

The Ct value for the NTC can be <35 for the following reasons:
- There are non-specific interactions between primers. For NTC information on a specific assay, see the Megaplex Assay Performance File.
- The cDNA template is contaminated. Please ollow established PCR good laboratory practices to prevent contamination.
- The preamplification product was not diluted properly before real-time PCR. Ensure that you dilute the preamplification product according the procedure in the user guide.

View all Megaplex™ PreAmp Primers, Human Pool A v2.1 FAQs