Applied Biosystems™

MeltDoctor™ HRM Calibration Plate, 384-well

Les plaques d’étalonnage Applied Biosystem® MeltDoctor™ HRM prêtes à l’emploi contiennent tous les composants requis pour la coloration pure etAfficher plus

Référence	Quantité
4425559	1 x 384-well plate

Référence 4425559

Prix (EUR)

868,00

Quantité:

1 x 384-well plate

Prix (EUR)

868,00

Les plaques d’étalonnage Applied Biosystem® MeltDoctor™ HRM prêtes à l’emploi contiennent tous les composants requis pour la coloration pure et l’étalonnage HRM, ce qui réduit la complexité des premiers pas dans l’expérimentation HRM.Ces plaques fournissent l’étalonnage thermique et optique nécessaire pour une discrimination précise et reproductible entre les courbes de fusion.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Colorant étalonnéColorant FHR (Fusion haute résolution)

Type d’étalonnageÉtalonnage thermique, étalonnage optique

À utiliser avec (application)PCR en temps réel (qPCR)

À utiliser avec (équipement)Système 7900HT Fast

FormatPlaque à 384 puits

Compatibilité des plaquesPlaque d’étalonnage

Gamme de produitsMeltDoctor

Quantité1 x 384-well plate

Conditions d’expéditionGlace carbonique

Type de produitPlaque d’étalonnage HRM

Unit SizeEach

Contenu et stockage

1 plaque, format 384 puits

La durée de conservation minimale garantie est de 60 jours (date de péremption exacte imprimée sur le produit et le certificat d’analyse).

Foire aux questions (FAQ)

How long should the amplicon be for HRM analysis?

We recommend to use 50-250 bp long PCR amplicons. Typically, shorter amplicons can distinguish the genotypes for a SNP better, especially for Type III and Type IV SNPs. This is simply because a single base variation affects the melting behavior of a 100 bp amplicon stronger than of a 500 bp amplicon, for example. In longer fragments, the risk of covering multiple mutations is also increased.

What can be used as positive control reactions in HRM analysis?

Positive control reactions should contain template DNA with a known sequence. In SNP genotyping experiments, this could be a sample with a known genotype. Positive control(s) for all genotypes should be included where possible to serve as a reference in melting curve comparison and assigning genotypes for test samples. In mutation scanning experiments, a sample with a wild type sequence could serve as a positive control. The controls should preferably have the same DNA concentration as their corresponding test samples. Control DNA should also be eluted and/or diluted in the same buffer as the samples.

In HRM analysis, when is it better to use a 3-step cycling protocol rather than a 2-step protocol?

A 3-step cycling protocol is recommended for the analysis of complicated (especially Type IV SNP) targets, amplicons longer than 200 bp, and amplicons with a primer annealing temperature that is less than 60 degrees C.

The HRM software will not accept my calibration file. What is wrong?

Check that there are no outliers on the plate. You cannot omit any wells on the HRM calibration plate.

I have a 7900HT Fast Real-Time PCR System, and cannot open the data file in HRM software. What could be causing this?

There are a few possibilities. First, make sure the HRM Software version is v2.0.1, and the 7900HT Fast Real-Time PCR System software version is v2.3 or above. Second, check that the run method used was as recommended in the HRM protocol; make sure the ramp rate for the dissociation stage is 1%. Then try to open the calibration file from the HRM Software; if it does not open, the calibration file is defective. The defects could be due to a bad calibration plate or instrument uniformity issue.

View all MeltDoctor™ HRM Calibration Plate, 384-well FAQs