The PrepSEQ™ Rapid Spin Sample Preparation Kit with Proteinase K is recommended for difficult to lyse bacteria like Listeria monocytogenes. The kit was developed for the rapid preparation of DNA from food and environmental samples for use in PCR amplification reactions. Using a simple spin protocol, PrepSEQ™ Rapid Spin efficiently inactivates PCR inhibitors. For laboratories involved in food and environmental testing, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. This has created the demand for a sample preparation method that is fast, cost-effective and capable of extracting high-quality DNA from a broad range of sample types. While such methods have been developed for dedicated, high-throughput laboratories, there is a growing need for methods suitable to low-to-medium throughput applications. The PrepSEQ™ Rapid Spin Kit with Proteinase K meets this need. • Designed for Food Safety and Environmental Monitoring applications • Supports low-to-medium throughput work loads (up to 100 samples a day) • Inactivates PCR inhibitors so you will most likely not have to repeat the template preparation step • Uses a simple spin protocol
Optimized for PCR-based Food Safety and Environmental Monitoring For laboratories involved in food safety and environmental monitoring, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. Use this sample preparation method for fast, cost-effective extraction of high-quality DNA from a broad range of sample types including chocolate, soft cheeses, and soil. Even samples with difficult-to-lyse bacteria, such as Listeria monocytogenes, produce excellent results with the addition of a Proteinase K digestion step.
Designed for Laboratories that Prepare up to 100 Samples a Day Kits exist for dedicated, high-throughput laboratories, but now you have a kit that is suitable for low-to-medium throughput applications. This kit is designed for laboratories that routinely prepare up to 100 samples a day.
Removes PCR Inhibitors The PrepSEQ™ Rapid Spin Sample Preparation Kit offers a simple, cost-effective way to prepare bacterial DNA from broth cultures. During the extraction process, the media inside the spin column removes PCR inhibitors from the sample, allowing the bacteria to pass through into the sample collection tube where it can be stored or used for PCR.
. For Research Use Only. Not for use in diagnostics procedures.
Spécifications
Format
Colonne de centrifugation
Isolation Technology
Colonne de centrifugation, filtration grossière, enzymatique
Type d’échantillon
Échantillons liquides (p. ex. le sérum), aliments & Échantillons environnementaux, culture bactérienne, bactéries (difficiles à lyser)
Type de produit final
ADN bactérien
À utiliser avec (équipement)
Centrifugeuse
À utiliser avec (application)
PCR quantitative en temps réel (qPCR), PCR
Compatibilité à haut débit
Non compatible avec le haut débit (manuel)
Nbre de réactions
100 réactions
Gamme de produits
PrepSEQ™
Cible
ADN génomique
Quantité de matériel de démarrage
750 µl
Contenu et stockage
Chaque kit contient 100 colonnes de centrifugation, 100 microtubes à centrifuger, un tampon de lyse (stocker à 4°C) et une protéinase K (stocker (à -20°C). 100 échantillons peuvent être préparés à l’aide de ce kit.)
Figures
Documentation et téléchargements
Certificats
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