Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Path-ID™ Multiplex One-Step Kit
Applied Biosystems™

Path-ID™ Multiplex One-Step Kit

Le kit de RT-PCR en une étape multiplex Path-ID™ est conçu pour la PCR multiplex de transcription inverse et quantitativeAfficher plus
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RéférenceNbre de réactions
4428206100 réactions
4428207500 réactions
44400221000 réactions
Référence 4428206
Prix (EUR)
399,00
Each
Ajouter au panier
Nbre de réactions:
100 réactions
Prix (EUR)
399,00
Each
Ajouter au panier
Le kit de RT-PCR en une étape multiplex Path-ID™ est conçu pour la PCR multiplex de transcription inverse et quantitative (qRT-PCR).Il est optimisé pour l’amplification simultanée de quatre cibles maximum à l’aide de vos échantillons d’ARN et de vos ensembles d’amorces⁄sondes TaqMan®.Les réactions sont exécutées à l’aide d’une procédure en une seule étape pour transcrire l’ARN et amplifier vos cibles.Le kit contient un mélange enzymatique multiplex avec transcriptase inverse Arrayscript™ et ADN polymérase AmpliTaq Gold®, ainsi qu’un mélange de tampons.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
À utiliser avec (équipement)Système 7000, système 7300, système 7500 Fast, système 7500, système 7700, Système 7900HT, système à 384 puits HT AB 7900, StepOne™, mode standard, StepOnePlus™, mode standard
FormatTube
Hot StartFonctionnalité Hot-Start intégrée
Nbre de réactions100 réactions
Colorant de référence passiveROX (pré-mélangé)
PolyméraseADN polymérase AmpliTaq Gold
Gamme de produitsPath-ID
Type de produitKits de RT-PCR en une étape
Quantité100 reactions
Transcriptase inverseArrayScript™
Type d’échantillonARN total, ARN viral, ARNm
Suffisant pour100 réactions
Méthode de détectionAmorce-sonde
À utiliser avec (application)Pathogen Detection, Virus Detection
FormeLiquide
Méthode PCR1-step RT-qPCR, Multiplex qPCR
Vitesse de réactionÉtalon
Unit SizeEach
Contenu et stockage
• 1.375 mL of 2X Multiplex RT-PCR Buffer
• 280 μL of 10X Multiplex Enzyme Mix including AmpliTaq Gold and ArrayScript
• 1.75 mL of Nuclease-free water
• Store the kit at -20°C; the Nuclease-free water may be stored at -20°C, 4°C, or room temperature

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

Foire aux questions (FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.