Path-ID™ Multiplex One-Step RT-PCR Kit
Path-ID™ Multiplex One-Step RT-PCR Kit
Applied Biosystems™

Path-ID™ Multiplex One-Step RT-PCR Kit

The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR). It is optimized for theAfficher plus
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RéférenceNbre de réactions
4442135100 réactions
4442136500 réactions
44421371000 réactions
Référence 4442135
Prix (EUR)
460,00
Each
Ajouter au panier
Nbre de réactions:
100 réactions
Prix (EUR)
460,00
Each
Ajouter au panier
The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR). It is optimized for the amplification of up to four targets simultaneously using your RNA samples and TaqMan™ primer-probe sets. Reactions are run using a single-tube, one-step procedure to reverse-transcribe the RNA and amplify your targets. The kit contains a Multiplex Enzyme Mix with Arrayscript™ reverse transcriptase and AmpliTaq Gold™ DNA Polymerase, and a buffer mix.
Spécifications
À utiliser avec (équipement)7500 Fast System, 7500 System
FormatTube
Hot StartBuilt-In Hot Start
Nbre de réactions100 réactions
Colorant de référence passiveROX (pré-mélangé)
PolyméraseAmpliTaq Gold DNA Polymerase
Gamme de produitsPath-ID
Type de produitMultiplex One-Step RT-PCR Kit
Quantité100 reactions
Transcriptase inverseArrayScript™ UP
Type d’échantillonARN
Suffisant pour100 réactions
Méthode de détectionAmorce-sonde
À utiliser avec (application)Pathogen Detection, Virus Detection
GC-Rich PCR PerformanceLow
Méthode PCR1-step RT-qPCR, Multiplex qPCR
Vitesse de réactionÉtalon
Unit SizeEach
Contenu et stockage
Store at -20°C. Includes 100 reactions with the following components: Multiplex RT-PCR Buffer, Multiplex Enzyme Mix, and Nuclease-free water.

Foire aux questions (FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.

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