Système de purification KingFisher™ Flex, KingFisher avec tête PCR à 96 puits

Citations et références (36)

Thermo Scientific™

Système de purification KingFisher™ Flex, KingFisher avec tête PCR à 96 puits

Les manipulateurs de particules magnétiques Thermo Scientific™ KingFisher™ Flex permettent de préparer efficacement des échantillons à partir de divers matériaux.

Référence	Quantité
5400610	Unité

Référence 5400610

Prix (EUR)

Quantité:

Unité

Préparez efficacement vos échantillons à partir de divers matériaux grâce au système de purification Thermo Scientific™ KingFisher™ Flex qui permet un traitement des particules magnétiques automatique et très polyvalent pour l’ADN/ARN, les protéines ou la purification des cellules à partir de pratiquement toutes les sources. Ce système, qui utilise une technologie révolutionnaire de séparation des particules magnétiques, permet une excellente reproductibilité et offre une excellente qualité.

Solution souple pour différents types de besoins de traitements d’échantillons. Avec 24 têtes aimantées, les clients peuvent monter le volume de traitement jusqu’à 5 ml, ce qui augmente le rendement de produit purifié. Pour les besoins de rendement plus élevés, 96 échantillons peuvent être traités avec une tête magnétique 96 puits.

Ce système entièrement automatisé offre une purification à grande vitesse des acides nucléiques, des protéines et des cellules.
Système à rendement élevé, il traite jusqu’à 96 échantillons en moins de 20 minutes.
Un système ouvert et souple qui permet au client d’utiliser tous les kits à base de particules magnétiques pour satisfaire les exigences des applications.
Le logiciel facile à utiliser Thermo Scientific™ BindIt™ permet de contrôler l’instrument, de créer et de modifier les protocoles.
Des protocoles prêts à l’emploi pour différents types d’applications sont disponibles.

Recommandées dans les cas suivants :

Génomique et protéomique
Identification de la cible
Dosages vétérinaires
Découverte des biomarqueurs
Contrôle qualité
Médecine légale

Pour un usage en laboratoire

Spécifications

Collection Efficiency of Particles> 95 %

Profondeur (impériale)23,6 in

Profondeur (métrique)60 cm

DescriptionPlaque KingFisher 96, plaque PCR

AffichageDÉMARRAGE / PAUSE / ARRÊT / OK / PLATEAU PIVOTANT DANS LE SENS HORAIRE / PLATEAU PIVOTANT DANS LE SENS ANTI-HORAIRE / quatre touches de déplacement / LCD

À utiliser avec (application)Génomique et protéomique, identification cible, dosages vétérinaires, découverte des marqueurs biologiques, contrôle de la qualité, médecine légale

Température de chauffageDe +5°C au-dessus de la température ambiante jusqu’à +115°C

Hauteur (impériale)15 in

Hauteur (métrique)38 cm

Tiges magnétiques96 dans un cadre ; têtes aimantées permutables

Taille des particules (métrique)environ >1 μm

Type de microplaquesPlaques de 24 ou 96 puits ; plaque de 24 puits profonds KingFisher (200 à 5 000 µl) ; plaque de microtitration de 96 puits profonds (50 à 1 000 µl) ; plaque de 96 puits KingFisher (20 à 200 µl) ; plaque PCR (20 à 100 µl)

QuantitéUnité

Poids (impériale)62 lb

Poids (métrique)28 kg

Largeur (impériale)26,8 in

Largeur (métrique)68 cm

Capacité96 échantillons

FormatPlaque 96 puits

Plaques par plateau24 ou 96

Volume (métrique) de traitement20 à 100 μl

Unit SizeEach

Foire aux questions (FAQ)

Where can I find the manual for the BindIt Software?

The User Manual for the BindIt Software can be downloaded from the BindIt Software product page online, under the Documents & Downloads section. It is also included within the BindIt Software installation file under "Documentation".

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

I cannot connect my KingFisher Flex instrument to the BindIt software. How do I troubleshoot this issue?

To determine the cause of the connection issue, we recommend checking the following:
1. Check software settings:
• Make sure the instrument selected is KingFisher Flex.
• Make sure the instrument serial number was input with the dash included and no spaces (ex, "711-xxxx").
• Make sure the communication tab was set to "USB" and not "COM".
2. Check the instrument and computer:
• The KingFisher Flex instrument screen (GUI) should be set to the "Factory Protocols" tab (blue tab) for best results.
• Make sure the port function on the Maintenance Tab (last tab) is checked for "USB".
• Try using different USB cables.
• Try a different USB port on the computer.
• Check the Windows version you are using.
• Reboot the KingFisher Flex instrument and the computer.
• Make sure the driver was installed properly. This may require the help of your local IT team.

If the connection issue persists, please contact our Technical Support team at techsupport@thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

What Uninterruptible Power Supply (UPS) do you recommend I use with the KingFisher Flex instrument?

You can use any brand of UPS as long as it is compatible with the following KingFisher Flex instrument electrical specifications:
• Mains power supply: 100–240 Vac, 50/60 Hz, nominal automatic voltage detection
• Power consumption: 175 VA max.; 55 VA standby

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

I am getting the following error message on the KingFisher Flex instrument: error message 12. What can I do to fix this error?

The “error message 12” error usually means there is something out of place on the KingFisher Flex instrument. We recommend the following to resolve the issue:
1. Check the heat block and make sure that it is not lifted up out of the base of the unit. If it is out of the base, check to see if there is anything underneath it that is preventing it from going down. If there is nothing under the heat block, it may have gotten stuck in the up position. This will require a service call.
2. Check that the tip shield is off to the right and out of the way from underneath the magnetic head. If it is stuck in the incorrect position, this may require a service call.
3. If the turntable was moved and it clicked out of place, the rectangular footprint cannot be centered below the head. Turn off the instrument and turn it back on. The instrument should re-align itself.

If the error continues to display, please contact techsupport@thermofisher.com for assistance.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

How do I use BindIt software 4.0 to load a script from my computer to the KingFisher Flex or KingFisher Duo/Duo Prime instrument?

Please follow these steps to load a script from your computer to the KingFisher Flex or KingFisher Duo/Duo Prime instrument:
1. Use the USB cable to connect the PC to the instrument.
2. Turn on the instrument.
3. Open the BindIt software and click the Connect button.
4. Select "Find New Instrument." BindIt will automatically connect to the instrument and the instrument screen will say "Computer Control".
5. Click the "Transfer" Button.
6. Click the "Upload" button.
7. Browse to find the file and double-click it.
8. In the new window that opens:
a. KingFisher Flex: you will be prompted to enter a new name – this is not necessary if the name only contains letters, digits, dash or underscore.
• If the name contains spaces, apostrophes or any other character, you should enter a new name containing only letters, digits, dash (-) or underscore (_).
• Click the "Category" pulldown to select the instrument folder you want to load the script to.
• Click OK.
b. KingFisher Duo/Duo Prime: The KingFisher Duo and Duo Prime instruments accept all characters in the filename.
• Click the "Category" pulldown to select the instrument folder you want to load the script to.
• Click OK.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

View all Système de purification KingFisher™ Flex, KingFisher avec tête PCR à 96 puits FAQs

Citations et références (36)

Citations et références

Abstract

Profiling cholinesterase adduction: a high-throughput prioritization method for organophosphate exposure samples.

Authors:Carter MD, Crow BS, Pantazides BG, Watson CM, DeCastro BR, Thomas JD, Blake TA, Johnson RC

Journal:

PubMed ID:23954929

'A high-throughput prioritization method was developed for use with a validated confirmatory method detecting organophosphorus nerve agent exposure by immunomagnetic separation high-performance liquid chromatography tandem mass spectrometry. A ballistic gradient was incorporated into this analytical method to profile unadducted butyrylcholinesterase (BChE) in clinical samples. With Zhang et al.''s Z'' factor ... More

Development and mechanism of ?-secretase modulators for Alzheimer's disease.

Authors:Crump CJ, Johnson DS, Li YM

Journal:Biochemistry

PubMed ID:23614767

'?-Secretase is an aspartyl intramembranal protease composed of presenilin, Nicastrin, Aph1, and Pen2 with 19 transmembrane domains. ?-Secretase cleaves the amyloid precursor proteins (APP) to release Aß peptides that likely play a causative role in the pathogenesis of Alzheimer's disease (AD). In addition, ?-secretase cleaves Notch and other type I ... More

Recommended mass spectrometry-based strategies to identify botulinum neurotoxin-containing samples.

Authors:Kalb SR, Baudys J, Wang D, Barr JR

Journal:

PubMed ID:25996606

'Botulinum neurotoxins (BoNTs) cause the disease called botulism, which can be lethal. BoNTs are proteins secreted by some species of clostridia and are known to cause paralysis by interfering with nerve impulse transmission. Although the human lethal dose of BoNT is not accurately known, it is estimated to be between ... More

Quantification of ricin, RCA and comparison of enzymatic activity in 18 Ricinus communis cultivars by isotope dilution mass spectrometry.

Authors:Schieltz DM, McWilliams LG, Kuklenyik Z, Prezioso SM, Carter AJ, Williamson YM, McGrath SC, Morse SA, Barr JR

Journal:

PubMed ID:25576235

'The seeds of the Ricinus communis (Castor bean) plant are the source of the economically important commodity castor oil. Castor seeds also contain the proteins ricin and R. communis agglutinin (RCA), two toxic lectins that are hazardous to human health. Radial immunodiffusion (RID) and the enzyme linked immunosorbent assay (ELISA) ... More

Using phage and yeast display to select hundreds of monoclonal antibodies: application to antigen 85, a tuberculosis biomarker.

Authors:Ferrara F, Naranjo LA, Kumar S, Gaiotto T, Mukundan H, Swanson B, Bradbury AR

Journal:PLoS One

PubMed ID:23166701

'Current diagnostic methods for tuberculosis (TB), a major global health challenge that kills nearly two million people annually, are time-consuming and inadequate. During infection a number of bacterial molecules that play a role in the infective process are released and have been proposed as biomarkers for early TB diagnosis. Antigen ... More

View all Système de purification KingFisher™ Flex, KingFisher avec tête PCR à 96 puits citations