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Thermo Scientific™
SMART Digest™ Dual Enzyme Columns for HDX-MS Analysis
Thermo Scientific™ SMART Digest™ Dual Enzyme Columns for HDX-MS analysis provide fast, reliable digestion of proteins for HDX-MS analysis, helping to provide the sequence characterization of biotherapeutics.
| Référence | Granulométrie |
|---|---|
| 60160-110 | ˜20 μm |
Référence 60160-110
Prix (EUR)
-
Granulométrie:
˜20 μm
Thermo Scientific SMART Digest Dual Enzyme columns containing pepsin and protease XIII provide fast, reliable digestion of proteins for for HDX-MS analysis helping to provide the sequence characterization of biotherapeutics.
SMART Digest Dual Enzyme columns for HDX-MS analysis help to provide the characterization of biotherapeutics.
The combination of enzymes are complementary, creating many short, overlapping N-terminal peptides for a high degree of sequence coverage.
Pepsin cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. It will not cleave at bonds containing valine, alanine or glycine. The optimum pH range for pepsin activity is 1.0–3.0.
Proteinase XIII cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH.
Spécifications
Format de colonneImmobilized enzyme
Type de colonneImmobilized enzyme in PEEK cartridge
DescriptionSMART Digest Pepsin/Protease XIII Dual Enzyme Column
Diamètre2.1 mm
Débit10 to 500 μL/min
À utiliser avec (application)HDX-MS analysis
Longueur30 mm
Pression max.2500 psi (170 bar)
Compatibilité de phase mobilePhosphate buffer pH 4
Plages de températures de fonctionnement4 °C to 50 °C
Matériau de remplissageHydrophilic/coated DVB
Granulométrie˜20 μm
Porosité° 1000 angstroms
Stabilité1 year
Phase stationnaireImmobilized enzyme
Température de stockageRT for up to 2 weeks; 4 °C for extended period (refrigerate in MES pH 6)
TechniqueBioLC
Poids8 g
CleavagePepsin: Cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. The optimum pH range for pepsin activity is 1.0–3.0.
Protease XIII: Cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH.
Protease XIII: Cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH.
EnzymesPepsin & Protease XIII
TypeSMART Digest Pepsin/Protease XIII Dual Enzyme column
pH2 to 6
Unit SizeEach