Microplaque à 384 puits, 240 μl, carrés à coins arrondis de diamètre 8 mm, fond en forme de U ; 6/boîte
Microplaque à 384 puits, 240 μl, carrés à coins arrondis de diamètre 8 mm, fond en forme de U ; 6/boîte
Thermo Scientific™

Microplaque à 384 puits, 240 μl, carrés à coins arrondis de diamètre 8 mm, fond en forme de U ; 6/boîte

Utilisez ces plaques à puits revêtues de verre pour une analyse à haut débit sur des échantillons nécessitant des flacons en verre.

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RéférenceVolume total (métrique) du puits
60180-P342252 μl
Référence 60180-P342
Prix (EUR)
-
Demander un devis
Volume total (métrique) du puits:
252 μl

Les échantillons qui nécessitent des flacons en verre peuvent désormais bénéficier d’une analyse à haut débit à l’aide de ces plaques à puits revêtues de verre. Nos plaques en plastique revêtues de verre Thermo Scientific™ WebSeal™ Plate+ de deuxième génération sont la solution aux plaques en plastique pur qui provoquent une mauvaise stabilité des solvants ou une perte de produit due à l’adsorption sur les parois des puits en plastique. Les produits Webseal Plate+ sont revêtus de verre à l’aide d’une technologie de pointe pour un revêtement à plus haute densité (épaisseur de 200 Å) afin d’améliorer la stabilité et la reproductibilité des solvants, en particulier pour les biomolécules qui s’adsorbent sur les parois en plastique.

  • Offre une adsorption ultra-faible sur la surface du puits pour les composés polaires critiques
  • Permet une analyse ultra-traces pour les analytes fortement adsorbants
  • Offre une reproductibilité optimale

Stabilité des solvants organiques supérieure
Les échantillons, qui ne peuvent normalement être manipulés que dans des flacons en verre, peuvent désormais bénéficier de la manipulation dans une plaque à puits à haut débit.

Moins d’adsorption sur la paroi de cavité pour les composés critiques
L’adsorption sur les parois de cavité est la raison n° 1 du nombre de résultats non reproductibles dans la bioanalyse. Lorsque la polarité de la paroi dans les plaques à puits Webseal Plate+ est inversée, le risque d’adsorption est considérablement réduit.

Reproductibilité accrue
Moins de risques de perte d’échantillons.

Spécifications
CertifiésSpecification Certified
Hauteur (métrique)22 mm
Plate SizeMid-well
Plage de températures (métrique)-80°C to 121°C
Volume total (métrique) du puits252 μl
Volume (métrique) de travail180 μl
Array16 x 24
CouleurClear
MatériauPolypropylène
Nbre de puits384
Gamme de produitsSureSTART WebSeal Level 3
StérilitéNon stérile
Traitement de surfaceGlass-coated
Type de puitsCoins arrondis
Unit SizePack of 6

Foire aux questions (FAQ)

Are bar-coded WebSeal plates available?

Yes, bar-coded WebSeal plates are available as a catalog product for the famly of Thermo Scientific Vanquish UHPLC machines only. However, plates can be provided with a pre-printed adhesive label applied. Contact your sales representative to assist with completing a form where you can define the bar code information and location of application onto the plate. Additional information required includes the physical requirements for the label such as temperature stability, solvent stability, etc.

What is the best way to seal a WebSeal Plate plus or plates with glass or PTFE inserts that need to be incubated at elevated temperatures?

WebSeal Plate+ or plates with glass or PTFE inserts should never be sealed with tapes or adhesive foils. Silicone mats should be used with the WebSeal Plate&plus products, preferably with a protective PTFE film to give increased chemical protection.

The glass inserted plates are sealed with specific silicone plug mats or individual caps. Again, most have additional PTFE films of layers to give increased chemical protection.

These plates, inserts, and mats were originally designed for combinatorial chemistry techniques with in situ chemical derivatization. Use with elevated temperatures is possible with the inserts and plugs. These are the closest plate format equivalent to glass autosampler vial products.

How do the Thermo Scientific-WebSeal Plate plus products compare to standard and certified products?

The WebSeal Plate+ coating provides additional surface protection that further reduces the possibility of interactions with solvents, sample component, or other materials. WebSeal Plate+ microptates display ultra-low background extractable organics profiles that compare favorably to our mass spectrometry certified vial products.

How do WebSeal-Certified low bleed plates compare to others?

Well plates are manufactured in molds. In order to ''force'' the ready produced plate to come out of this mold for the next shot, some manufacturers use release agents (organic compounds that act as lubricants). These release agents can be detected in your chromatogram. The grade of polypropylene used by most plate manufacturers can contain monomers or other added ingredients that are susceptible to leaching on contact with organic solvents. WebSeal-Certified products are manufactured from high purity resins and use a process that does not require mold release agents and that is why they exhbit no significant background extractables.

Do standard WebSeal plates bleed with organic solvents, or is it better to purchase certified plates?

If you are using high concentrations of organic solvents in your sample, then you may be concerned about ''bleed''. This is a term which normally implies a constant loss of material from the column or separation system which leads to high background levels, increased noise, and loss of sensitivity. If using solutions with a high level of buffer or water, then this should not be a concern as such components will not leach under these conditions. All plates may be used.

The evaluation of the plastic resins used in the production of plates was also carried out by controlled extraction of leachable components with organic solvents associated with HPLC and GC operation. These included methanol, acetonitrile, and hexane. Standard plates showed low level of extractables when tested at the highest instrument sensitivity range. These extractables found are characteristic of the common materials used for microplates in the clinical market and plates that have been used for general purpose chromatography applications. This indicates that for many non-critical applications and with higher concentrations of sample, the standard quality plates will be acceptable. These plates are termed Chromatography Tested.

The very low levels of background extractables found during our testing of some plates showed for applications run at more sensitive instrument settings. Where higher purity of the plate is required, you should directly switch to the Certified plates as they are the lowest extraction PP plates currently available on the world market. For critical analysis with high organic solvent concentrations and low sample concentrations, the Certified product range should be the plates of choice.