PBS (10X), pH de 7,4
Gibco™

PBS (10X), pH de 7,4

La solution saline dans un tampon phosphate (PBS) est une solution saline équilibrée qui est utilisée pour une grande variétéAfficher plus
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RéférenceQuantité
70011044500 mL
Référence 70011044
Prix (EUR)
93,25
Each
Quantité:
500 mL
Prix (EUR)
93,25
Each
La solution saline dans un tampon phosphate (PBS) est une solution saline équilibrée qui est utilisée pour une grande variété d’applications de culture cellulaire, telles que le lavage des cellules avant dissociation, le transport des cellules et des tissus, la dilution des cellules pour comptage et la préparation des réactifs. La solution PBS est formulée sans calcium ni magnésium pour rincer les chélateurs de la culture avant la dissociation des cellules. Les formes concentrées de PBS Gibco™ peuvent nécessiter un ajustement du pH au moment de la dilution (voir le protocole pour plus de détails).

Nous proposons une variété de formulations de PBS Gibco™ pour une vaste gamme d’applications de culture cellulaire.

Cette solution PBS est fabriquée comme suit :

Sans
• Calcium
• Magnésium
• Rouge de phénol

La formulation complète est disponible.

Système de fabrication et de Qualité BPFa
Gibco™ PBS est fabriqué dans un établissement conforme aux BPFa, situé à Grand Island, dans l’État de New York. Ce site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485. Pour assurer la continuité de la chaîne d’approvisionnement, nous proposons un produit PBS Gibco™ identique fabriqué sur notre site situé en Écosse (70011-036). Ce site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485.
À des fins de recherche ou de commercialisation avancée Non destiné aux diagnostics ou à l’administration directe à des humains ou des animaux.
Spécifications
Nom chimique ou matériauSolution saline dans un tampon phosphate
CouleurBlancs
ComprendSans pyruvate de sodium
Qualité de fabricationcGMP-compliant under the ISO 13485 standard
Osmolalité2800 à 3100 mOsm/kg
Température de stockageConditions de stockage : 15°C à 30°C
Conditions d’expédition : température ambiante
Durée de conservation : 24 mois à compter de la date de fabrication
StérilitéStérilisation par filtration
Concentration10X
À utiliser avec (application)Culture de cellules de mammifères
Forme physiqueLiquide
Gamme de produitsGibco
Quantité500 mL
Type de solutionSolution saline dans un tampon phosphate
pH7,4
Unit SizeEach

Foire aux questions (FAQ)

Are your PBS products tested for endotoxin levels?

Our PBS, pH 7.2 and PBS, pH 7.4 products are tested for endotoxin and the endotoxin levels are shown on the Certificate of Analysis (CoA) for the particular lot number of the product.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your PBS products sterile and suitable for cell culture?

We offer PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free (Cat. Nos. AM9624 and AM9625), which is molecular biology grade and is certified to be RNase-free. However, it is not sterile and hence is not cell culture-grade.

All our other PBS products (Cat. Nos. 70011xxx, 10010xxx, 70013xxx, 20012xxx) are filter-sterilized using a 0.1 µm filter and are cell culture grade (ready to use directly on cells). However, they are not tested for nuclease activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your PBS products DNase- and RNase-free?

We offer PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free (Cat. Nos. AM9624 and AM9625) that is molecular biology grade and is certified to be RNase-free. Our other PBS products are made with distilled water and are filter-sterilized, but are not tested for RNase and DNase activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is a precipitate in PBS normal?

No, this is not normal and if there is a precipitate in the product, it cannot be used. If the precipitate will not go back into solution, please contact Technical Support at techsupport@thermofisher.com for a replacement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between PBS and DPBS?

PBS has no potassium chloride (KCl) and it has a slightly lower level of phosphate (PO4) than DPBS. PBS is used for washing cells when viability isn't critical.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations et références (4)

Citations et références
Abstract
Subcellular targeting of RGS9-2 is controlled by multiple molecular determinants on its membrane anchor, R7BP.
Authors:Song JH, Waataja JJ, Martemyanov KA,
Journal:J Biol Chem
PubMed ID:16574655
'RGS9-2, a member of the R7 RGS protein family of neuronal RGS (Regulators of G protein Signaling), is a critical regulator of G protein signaling. In striatal neurons, RGS9-2 is tightly associated with a novel palmitoylated protein - R7BP (R7 family Binding Protein). Here we report that R7BP acts to ... More
Inhibition of transforming growth factor beta signaling and Smad-dependent activation of transcription by the Latent Membrane Protein 1 of Epstein-Barr virus.
Authors: Prokova Vassiliki; Mosialos George; Kardassis Dimitris;
Journal:J Biol Chem
PubMed ID:11781310
'Inhibition of transforming growth factor beta (TGFbeta) signaling by the Epstein-Barr virus Latent Membrane Protein 1 (LMP1) may account, at least in part, for the oncogenic activity of LMP1. We found that LMP1 is a potent inhibitor of TGFbeta signaling and Smad-dependent activation of transcription in 293 epithelial cells and ... More
Stiffness-controlled three-dimensional extracellular matrices for high-resolution imaging of cell behavior.
Authors:Fischer RS, Myers KA, Gardel ML, Waterman CM,
Journal:Nat Protoc
PubMed ID:23099487
Regulation of cell functions by the physical properties of the extracellular matrix (ECM) has emerged as a crucial contributor to development and disease. Two specific physical properties of the ECM, stiffness and dimensionality, each influence cell signaling and function. As these ECM physical properties are linked to other properties that ... More
Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures.
Authors:Zhang F, Gradinaru V, Adamantidis AR, Durand R, Airan RD, de Lecea L, Deisseroth K,
Journal:Nat Protoc
PubMed ID:20203662
Elucidation of the neural substrates underlying complex animal behaviors depends on precise activity control tools, as well as compatible readout methods. Recent developments in optogenetics have addressed this need, opening up new possibilities for systems neuroscience. Interrogation of even deep neural circuits can be conducted by directly probing the necessity ... More