This Anti-Human Foxp3 Staining Set contains the buffers and monoclonal antibody necessary to successfully stain and identify Foxp3 cells. The 236A/E7 monoclonal antibody reacts with human Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3 is a 49-55 kDa protein and a member of the forkhead/winged-helix family of transcription factors. It was identified as the gene responsible for the X-linked lymphoproliferative disease observed in scurfy (sf) mice and in the human disorder, X-linked autoimmunity-allergic dysregulation syndrome (XLAAD). Constitutive expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

The 236A/E7 antibody has been shown to crossreact with rhesus macaque, sooty mangabey, and cynomolgus Foxp3 236A/E7 recognizes a different epitope of Foxp3 than PCH101 and 150D/E4.

Isotype
IgG1

Laser
Blue Laser

Emit
575 nm

Excite
488 nm

Reported Application
Intracellular Staining Followed by Flow Cytometric Analysis