Cocktail inhibiteur de protéase et de phosphatase Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
Thermo Scientific™

Cocktail inhibiteur de protéase et de phosphatase Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Le cocktail inhibiteur de protéase et de phosphatase Thermo Scientific Halt (100X) offre une solution pratique et unique avec uneAfficher plus
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RéférenceQuantité
784401 ml
7844224 x 100 μl
7844610 ml
784445 x 1 mL
Référence 78440
Prix (EUR)
286,65
Precio exclusivo en nuestra web
349,00
Économisez 62,35 (18%)
Each
Quantité:
1 ml
Grand volume ou format personnalisé
Prix (EUR)
286,65
Precio exclusivo en nuestra web
349,00
Économisez 62,35 (18%)
Each
Le cocktail inhibiteur de protéase et de phosphatase Thermo Scientific Halt (100X) offre une solution pratique et unique avec une protection complète des échantillons protéiques pour les lysats de cellules et de tissus.

Caractéristiques du cocktail inhibiteur de protéase et de phosphatase Halt (100X) :

Protection complète : le cocktail tout-en-un contient des inhibiteurs de protéases et de phosphatases
Plusieurs formats de conditionnement : faites votre choix parmi quatre tailles de conditionnement pratiques pour convenir à pratiquement toutes les échelles
Pratique : la formulation liquide 100X est bien plus facile et plus efficace à utiliser que les inhibiteurs individuels et d’autres formats
Stockage facile : le format à base aqueuse permet un stockage pratique en chambre froide sans congélation, contrairement aux cocktails à base de DMSO
Compatible : peut être utilisé en spectrométrie de masse (MS) parce qu’il ne contient pas d’AEBSF, qui peut causer un déplacement des pics
Validé : entièrement compatible avec les tampons de lyse cellulaire Thermo Scientific Pierce

Le cocktail inhibiteur de protéases et de phosphatases Halt est spécifiquement optimisé pour protéger les protéines contre la dégradation au cours de l’extraction et de la purification. Le cocktail contient des inhibiteurs contre les principales classes de protéases et de phosphatases, ciblant les aminopeptidases, les protéases à cystéine et les sérines protéases, ainsi que les sérines/thréonines phosphatases et les protéines tyrosines phosphatases. Un tube contenant de l’EDTA à 0,5 M est fourni séparément pour promouvoir l’inhibition des métalloprotéases.

Produits associés
™ Cocktail inhibiteur de protéases et de phosphatases Halt, sans EDTA (100X)
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
TamponTampon de lyse
InhibiteursInhibiteur de protéase, inhibiteur de phosphatase
Quantité1 ml
Type de réactifInhibiteur de protéase, inhibiteur de phosphatase
FormeLiquide
Gamme de produitsHalt
Type de produitCocktail inhibiteur
Unit SizeEach
Contenu et stockage
Dès réception, stocker à 4°C

Foire aux questions (FAQ)

Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?

The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?

Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?

EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?

The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations et références (7)

Citations et références
Abstract
Deglycosylation of pathological specimens alters performance of diagnostic PDL1 antibodies.
Authors:Dressler FF,Dabadghao DS,Klapper L,Perner S,Idel C,Ribbat-Idel J
Journal:Virchows Archiv : an international journal of pathology
PubMed ID:35779078
Immunohistochemical (IHC) predictive quantitation of PDL1 expression is obligatory in many cancer entities with improved response to immune checkpoint inhibition in PDL1-positive subgroups. With recent demonstration of increased positivity rates after enzymatic deglycosylation in breast cancer specimens, a comparative analysis with two different antibodies and extended controls was performed in ... More
Imlunestrant Is an Oral, Brain-Penetrant Selective Estrogen Receptor Degrader with Potent Antitumor Activity in ESR1 Wild-Type and Mutant Breast Cancer.
Authors:Bhagwat SV,Mur C,Vandekopple M,Zhao B,Shen W,Marugán C,Capen A,Kindler L,Stephens JR,Huber L,Castanares MA,Garcia-Tapia D,Cohen JD,Bastian J,Mattioni B,Yuen E,Baker TK,Rodriguez Cruz V,Fei D,Manro JR,Pulliam N,Dowless MS,Ortiz Ruiz MJ,Yu C,Puca L,Klippel A,Bacchion F,Ismail-Khan R,Rodrik-Outmezguine V,Peng SB,Lallena MJ,Gong X,de Dios A
Journal:Cancer research
PubMed ID:39652577
Targeting of the estrogen receptor (ER) by antiestrogens is the standard of care for patients with ER+ HER2- advanced/metastatic breast cancer. Although antiestrogens that degrade ERα (fulvestrant) or block estrogen production (aromatase inhibitors) have improved patient outcomes, clinically important challenges remain related to drug administration, limited bioavailability, lack of brain ... More
Urologic pharmacology, Part I.
Authors:
Journal:Seminars in urology
PubMed ID:3843699
hCLCA1 and mCLCA3 are secreted non-integral membrane proteins and therefore are not ion channels.
Authors:Gibson A, Lewis AP, Affleck K, Aitken AJ, Meldrum E, Thompson N
Journal:J Biol Chem
PubMed ID:15919655
'Proteins of the CLCA gene family have been proposed to mediate calcium-activated chloride currents. In this study, we used detailed bioinformatics analysis and found that no transmembrane domains are predicted in hCLCA1 or mCLCA3 (Gob-5). Further analysis suggested that they are globular proteins containing domains that are likely to be ... More
Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.
Authors:Ståhlberg A, Thomsen C, Ruff D, Åman P
Journal:Clin Chem
PubMed ID:23014600
The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. ... More