Cocktail d’inhibiteurs de protéase et de phosphatase Halt ™, exempt d’EDTA (100X)

Citations et références (5)

Thermo Scientific™

Cocktail d’inhibiteurs de protéase et de phosphatase Halt ™, exempt d’EDTA (100X)

Name: Cocktail d’inhibiteurs de protéase et de phosphatase Halt ™, exempt d’EDTA (100X)
SKU: 78443
Price: 395.65 EUR

Le cocktail inhibiteur de protéases et de phosphatases Thermo Scientific Halt, exempt d’EDTA (100X), fournit le confort d’une solution simpleAfficher plus

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Référence	Quantité
78443	24 x 100 μl
78441	1 ml
78445	5 x 1 mL
78447	10 ml

4 options

Référence 78443

Prix (EUR)

395,65

線上優惠

466,00

Économisez 70,35 (15%)

Each

Quantité:

24 x 100 μl

Grand volume ou format personnalisé

Prix (EUR)

395,65

線上優惠

466,00

Économisez 70,35 (15%)

Each

Le cocktail inhibiteur de protéases et de phosphatases Thermo Scientific Halt, exempt d’EDTA (100X), fournit le confort d’une solution simple avec une protection complète des échantillons de protéines pour les lysats cellulaires et tissulaires.

Caractéristiques du cocktail inhibiteur de protéases et de phosphatases Halt, exempt d’EDTA (100X) :

• Protection complète : le cocktail tout-en-un contient des inhibiteurs de protéases et de phosphatases
• Plusieurs formats de conditionnement : faites votre choix parmi quatre tailles de conditionnement pratiques pour convenir à pratiquement toutes les échelles
• Pratique : la formulation liquide 100X est bien plus facile et plus efficace à utiliser que les inhibiteurs individuels et d’autres formats
• Stockage facile : le format à base aqueuse permet un stockage pratique en chambre froide sans congélation, contrairement aux cocktails à base de DMSO
• Compatible : peut être utilisé en spectrométrie de masse (MS) parce qu’il ne contient pas d’AEBSF, qui peut causer un déplacement des pics
• Validé : entièrement compatible avec les tampons de lyse cellulaire Thermo Scientific Pierce

Le cocktail inhibiteur de protéases et de phosphatases Halt est spécifiquement optimisé pour protéger les protéines contre la dégradation au cours de l’extraction et de la purification. Le cocktail contient des inhibiteurs contre les principales classes de protéases et de phosphatases, ciblant les aminopeptidases, les protéases à cystéine et les sérines protéases, ainsi que les sérines/thréonines phosphatases et les protéines tyrosines phosphatases.

Produits associés
Cocktail inhibiteur de protéases et de phosphatases Halt™ (100X)

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

TamponTampon de lyse

DescriptionCocktail inhibiteur de protéases et de phosphatases Halt, exempt d’EDTA

InhibiteursInhibiteur de protéase, inhibiteur de phosphatase

Quantité24 x 100 μl

Type de réactifInhibiteur de protéase, inhibiteur de phosphatase

FormeLiquide

Gamme de produitsHalt

Type de produitCocktail inhibiteur

Unit SizeEach

Contenu et stockage

Dès réception, stocker à 4°C

Foire aux questions (FAQ)

Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?

The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?

Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?

EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?

The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Cocktail d’inhibiteurs de protéase et de phosphatase Halt ™, exempt d’EDTA (100X) FAQs

Citations et références (5)

Citations et références

Abstract

Proteomic profiling across breast cancer cell lines and models.

Authors:Kalocsay M,Berberich MJ,Everley RA,Nariya MK,Chung M,Gaudio B,Victor C,Bradshaw GA,Eisert RJ,Hafner M,Sorger PK,Mills CE,Subramanian K

Journal:Scientific data

PubMed ID:37542042

We performed quantitative proteomics on 60 human-derived breast cancer cell line models to a depth of ~13,000 proteins. The resulting high-throughput datasets were assessed for quality and reproducibility. We used the datasets to identify and characterize the subtypes of breast cancer and showed that they conform to known transcriptional subtypes, ... More

Sting and p53 DNA repair pathways are compromised in Alzheimer's disease.

Authors:Nelson TJ,Xu Y

Journal:Scientific reports

PubMed ID:37221295

Alzheimer's disease (AD) is the most common cause of dementia. A common finding in AD is DNA damage. Double-strand DNA breaks (DSBs) are particularly hazardous to neurons because their post-mitotic state forces neurons to rely on error-prone and potentially mutagenic mechanisms to repair DNA breaks. However, it remains unclear whether ... More

FMRP S499 is phosphorylated independent of mTORC1-S6K1 activity.

Authors:Bartley CM,O'Keefe RA,Bordey A

Journal:PloS one

PubMed ID:24806451

Hyperactive mammalian target of rapamycin (mTOR) is associated with cognitive deficits in several neurological disorders including tuberous sclerosis complex (TSC). The phosphorylation of the mRNA-binding protein FMRP reportedly depends on mTOR complex 1 (mTORC1) activity via p70 S6 kinase 1 (S6K1). Because this phosphorylation is thought to regulate the translation ... More

Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.

Authors:Ståhlberg A, Thomsen C, Ruff D, Åman P

Journal:Clin Chem

PubMed ID:23014600

The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. ... More

The Src inhibitor dasatinib accelerates the differentiation of human bone marrow-derived mesenchymal stromal cells into osteoblasts.

Authors:Id Boufker H, Lagneaux L, Najar M, Piccart M, Ghanem G, Body JJ, Journé F

Journal:BMC Cancer

PubMed ID:20565769

The proto-oncogene Src is an important non-receptor protein tyrosine kinase involved in signaling pathways that control cell adhesion, growth, migration and differentiation. It negatively regulates osteoblast activity, and, as such, its inhibition is a potential means to prevent bone loss. Dasatinib is a new dual Src/Bcr-Abl tyrosine kinase inhibitor initially ... More