Kit de dosage ViewRNA™ Cell Plus

Citations et références (10)

Invitrogen™

Kit de dosage ViewRNA™ Cell Plus

Le dosage ViewRNA Cell Plus est un nouveau dosage qui associe l’immunocytochimie à la technologie ViewRNA, une technique exclusive d’hybridationAfficher plus

Référence	Quantité
88-19000-99	1 kit

Référence 88-19000-99

Prix (EUR)

1 966,00

Ajouter au panier

Quantité:

1 kit

Prix (EUR)

1 966,00

Ajouter au panier

Le dosage ViewRNA Cell Plus est un nouveau dosage qui associe l’immunocytochimie à la technologie ViewRNA, une technique exclusive d’hybridation in situ fluorescente et d’amplification séquentielle de l’ADN ramifié, pour visualiser à la fois l’ARN, avec une sensibilité d’une molécule, et la protéine dans chaque cellule. Ce dosage permet de détecter simultanément jusqu’à trois cibles d’ARN en association avec l’immunophénotypage pour les protéines de surface cellulaire ou intracellulaires à l’aide de l’immunocytochimie directe et indirecte pour permettre une caractérisation détaillée des sous-populations cellulaires spécifiques. Pour la détection simultanée d’une quatrième cible d’ARN, le module ViewRNA ISH Cell 740 (n° de cat. QVC0200) peut être associé à ce kit. Il permet l’analyse d’une cible supplémentaire dans le canal 740 (AlexaFluor 750).

Ce kit de dosage ViewRNA Cell Plus contient tous les réactifs nécessaires à la réalisation du dosage. Les kits de sondes cibles pour les gènes d’intérêt et les anticorps sont vendus séparément.

Réactivité / espèces
Les sondes peuvent être conçues pour n’importe quelle espèce. Les espèces les plus fréquemment utilisées / testées sont les suivantes : hamster arménien, babouin, bovin, chien, chat, poulet, chimpanzé, vache, singe cynomolgus, âne, chèvre, hamster syrien doré, cobaye, hamster, cheval, humain, macaque, singe, souris, primate non humain, babouin olive, cochon, macaque à queue de cochon, lapin, rat, singe rhésus, mouton

Application rapportée
Microscopie, immunocytochimie

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

À utiliser avec (application)Microscopie, immunocytochimie

Type de produitKit d’analyse d’hybridation ARN in situ

Quantité1 kit

Méthode de détectionDétection amorce-sonde

FormatKit

Unit SizeEach

Foire aux questions (FAQ)

How do ViewRNA assays compare to RNAScope assays?

ViewRNA and RNAScope technologies rely on the same signal amplification strategy - branched DNA amplification. Historically, both ViewRNA and RNAScope technologies originated from the same company, Panomics. The assays are expected to yield similar sensitivity and resolution, however each technology relies on its own set of proprietary reagents and probe set designs. Hence, the assays are not considered interchangeable or compatible. ViewRNA probe sets are not tested for RNAScope assaya and vice versa.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is the signal amplified in ViewRNA assays?

The ViewRNA technology relies on branched DNA signal amplification strategy. Target probes complementary to the target transcript sequence are further hybridized with pre-amplifier, amplifier and label probes that consist of branched DNA, and form 'tree branches' that allow numerous label probes to attach. This approach allows higher signal amplification compared to traditional ISH techniques.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Where can I find general information about ViewRNA ISH Assays?

For general information about ViewRNA ISH Assays, please go to this page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/in-situ-hybridization-ish/rna-fish/viewrna-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For ViewRNA assays, can I use the same set of wash solutions for all samples, including the positive and negative controls?

We do not recommend doing this. The negative control should be processed and washed separately from the rest of the samples. This is because the negative control does not contain any target probe sets and only the amplification reagents are added to it. If experimental samples are washed in the same beaker of wash solutions as the negative control, any unbound target probes that wash away can carry over to the negative control sample and cause unexpected positive signal (that will also appear to be very specific).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

To perform ViewRNA assays, which incubator oven should I use, Cat. No. QS0704 or QS0712?

Cat. No. QS0704 is a 120 V unit, for use in US/Canada region (https://www.thermofisher.com/order/catalog/product/QS0704).

Cat. No. QS0712 is a 220 V unit, for use in European region (https://www.thermofisher.com/order/catalog/product/QS0712).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Kit de dosage ViewRNA™ Cell Plus FAQs

Citations et références (10)

Citations et références

Abstract

Single cell transcriptome profiling of retinal ganglion cells identifies cellular subtypes.

Authors:Rheaume BA, Jereen A, Bolisetty M, Sajid MS, Yang Y, Renna K, Sun L, Robson P, Trakhtenberg EF

Journal:Nat Commun

PubMed ID:30018341

'Retinal ganglion cells (RGCs) convey the major output of information collected from the eye to the brain. Thirty subtypes of RGCs have been identified to date. Here, we analyze 6225 RGCs (average of 5000 genes per cell) from right and left eyes by single-cell RNA-seq and classify them into 40 ... More

miR-103 promotes endothelial maladaptation by targeting lncWDR59.

Authors:Natarelli L, Geißler C, Csaba G, Wei Y, Zhu M, di Francesco A, Hartmann P, Zimmer R, Schober A

Journal:Nat Commun

PubMed ID:29980665

'Blood flow at arterial bifurcations and curvatures is naturally disturbed. Endothelial cells (ECs) fail to adapt to disturbed flow, which transcriptionally direct ECs toward a maladapted phenotype, characterized by chronic regeneration of injured ECs. MicroRNAs (miRNAs) can regulate EC maladaptation through targeting of protein-coding RNAs. However, long noncoding RNAs (lncRNAs), ... More

Long non-coding RNAs influence the transcriptome in pulmonary arterial hypertension: the role of PAXIP1-AS1.

Authors:Jandl K, Thekkekara Puthenparampil H, Marsh LM, Hoffmann J, Wilhelm J, Veith C, Sinn K, Klepetko W, Olschewski H, Olschewski A, Brock M, Kwapiszewska G

Journal:J Pathol

PubMed ID:30450722

'In idiopathic pulmonary arterial hypertension (IPAH), global transcriptional changes induce a smooth muscle cell phenotype characterised by excessive proliferation, migration, and apoptosis resistance. Long non-coding RNAs (lncRNAs) are key regulators of cellular function. Using a compartment-specific transcriptional profiling approach, we sought to investigate the link between transcriptional reprogramming by lncRNAs ... More

Detection and Differentiation of Multiple Viral RNAs Using Branched DNA FISH Coupled to Confocal Microscopy and Flow Cytometry.

Authors:van Buuren N, Kirkegaard K

Journal:Bio Protoc

PubMed ID:30505886

Due to the exceptionally high mutation rates of RNA-dependent RNA polymerases, infectious RNA viruses generate extensive sequence diversity, leading to some of the lowest barriers to the development of antiviral drug resistance in the microbial world. We have previously discovered that higher barriers to the development of drug resistance can ... More

KDM6A and KDM6B play contrasting roles in nuclear transfer embryos revealed by MERVL reporter system.

Authors:Yang L, Song L, Liu X, Bai L, Li G

Journal:EMBO Rep

PubMed ID:30389724

Despite the success of animal cloning by somatic cell nuclear transfer (SCNT) in many species, the method is limited by its low efficiency. After zygotic genome activation (ZGA) during mouse development, a large number of endogenous retroviruses (ERVs) are expressed, including the murine endogenous retrovirus-L (MuERVL/MERVL). In this study, we ... More

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