Nitrocellulose Membranes, 0.2 μm
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Thermo Scientific™

Nitrocellulose Membranes, 0.2 μm

Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (western blotting, dot-blot assays, and other protein or nucleic acid methods).
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RéférenceDimensions (LxW)Suffisant pour
880137,9 cm x 10,5 cm15 transferts par mini-gel
880248 x 8 cm15 transferts par mini-gel
770128 x 12 cm25 transferts sur mini-gel
Référence 88013
Prix (EUR)
201,00
Each
Ajouter au panier
Dimensions (LxW):
7,9 cm x 10,5 cm
Suffisant pour:
15 transferts par mini-gel
Grand volume ou format personnalisé
Prix (EUR)
201,00
Each
Ajouter au panier
Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (western blotting, dot-blot assays, and other protein or nucleic acid methods). The 0.2-μm pore size is well-suited for western blotting, and this smaller pore size is ideal for the transfer of low molecular weight proteins or peptides and nucleic acids (<300 bp).

Features include:
100% pure nitrocellulose membrane
• Convenient—save time with pre-cut sheets
• Compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
• High-binding affinity—provides excellent protein binding, blocks easily, and does not require pre-wetting with alcohol
• Low background—produce high-quality data with low background signal in both chemiluminescent and fluorescent western blotting

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
DescriptionMembrane en nitrate de cellulose Pierce, 0,2 µm, 7,9 cm x 10,5 cm
Quantité15 feuilles
Conditions d’expéditionTempérature ambiante
Dimensions (LxW)7,9 cm x 10,5 cm
FormatFeuille
Longueur (métrique)10,5 cm
MatériauNitrocellulose
Porosité0,2 μm
Suffisant pour15 transferts par mini-gel
Largeur (métrique)7,9 cm
Unit SizeEach
Contenu et stockage
Conservez les membranes à plat à température ambiante, à l’abri des vapeurs chimiques. Certaines vapeurs de solvants peuvent partiellement dissoudre les membranes, ce qui perturbe la structure des pores. Mettez les membranes à l’abri de la lumière directe du soleil. La lumière du soleil peut provoquer la sécheresse du nitrate de cellulose et le rendre fragile. Remarque : Les filtres à membrane en nitrate de cellulose sont très inflammables. Tenez-les à l’écart de la chaleur, des étincelles et des flammes nues Le point d’éclair est d’environ 2°C. Les membranes peuvent être stérilisées par autoclavage à la vapeur à 121°C.

Foire aux questions (FAQ)

How can I store, strip, and reuse my western blot?

For nitrocellulose or PVDF membrane following Western blot detection using a chemiluminescent or fluorescent substrate system: Following transfer, air dry the membrane and place in an envelope, preferably on top of a supported surface to keep the membrane flat. The blot can be stored indefinitely at -80 degrees C. When ready to reprobe, prewet the PVDF blot with alcohol for a few seconds, followed by a few rinses with pure water to reduce the alcohol concentration. Then proceed as normal with blocking step.

FOR STRIPPING/REPROBING OF MEMBRANES: Harsh protocol (see NOTE below for modifications)

1) Submerge the membrane in stripping buffer (100 mM BME, 2% SDS, 62.5 mM Tris-HCl, pH 6.7) and incubate at 50 degrees C for 30 min with occasional agitation. If more stringent conditions necessary, incubate at 70 degrees C.

2) Wash 2 x 10 min in TBS-T/PBS-T at room temperature.

3) Block the membrane by immersing in 5% blocking reagent TBS-T or PBS-T for 1 hr at room temperature.

4) Immunodetection

NOTE: Often you don't need such harsh conditions to remove antibodies from their proteins. The stringency of one or several of the variables can be decreased: lower the temperature, decrease the time, less BME, less SDS, etc. An especially mild but still often effective stripping protocol is lower pH incubation. Example: pH 2.0 Tris 50-100 mM, 30-60 min incubation (you may do two incubations if you wish). Then rinse and block as usual. If you do not wish to re-use the membrane immediately after stripping, you can store the membrane in plastic wrap (wet, you do not want it to dry out). Another simple, mild stripping buffer is 0.1 M glycine•HCl (pH 2.5-3.0), incubation 30 min to 2 hrs room temperature or 37 degrees C, depending on the antibody.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are your PVDF and nitrocellulose membranes compatible with the Li-COR instrument?

Yes, both our PVDF and nitrocellulose membranes are compatible with the Li-COR instrument.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.