ARP (<i>N</i>-(aminooxyacetyl)-<i>N</i>'-(D-Biotinoyl) hydrazine, Trifluoroacetic Acid Salt)

Citations et références (18)

Invitrogen™

ARP (N-(aminooxyacetyl)-N'-(D-Biotinoyl) hydrazine, Trifluoroacetic Acid Salt)

Hydrazine ARP (N-(aminoxyacétyl)-N’-(D-biotinoyl), sel d’acide trifluoroacétique)

Référence	Quantité
A10550	10 mg

Référence A10550

Prix (EUR)

508,00

Quantité:

10 mg

Prix (EUR)

508,00

L’ARP (sonde d’aldéhyde-réactif) est une hydroxylamine biotinylée qui réagit avec le groupe d’aldéhyde exposé formé au niveau des sites abasiques dans de l’ADN endommagé. Les biotines liées aux sites abasiques peuvent ensuite être quantifiées avec des complexes de streptavidine fluorescents ou conjugués à des enzymes. Le protocole ARP convient aux dosages sur microplaques.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Réactivité chimiqueAcide carboxylique, cétone, aldéhyde

Marqueur ou colorantBiotine

Type de produitARP

Quantité10 mg

Groupement de réactifsAmine, hydroxylamine

Conditions d’expéditionTempérature ambiante

Type d’étiquetteBiotine et autres haptènes

Unit SizeEach

Contenu et stockage

Conserver au congélateur (-5 à -30°C).

Citations et références (18)

Citations et références

Abstract

Optimizing antibody immobilization strategies for the construction of protein microarrays.

Authors:Peluso P, Wilson DS, Do D, Tran H, Venkatasubbaiah M, Quincy D, Heidecker B, Poindexter K, Tolani N, Phelan M, Witte K, Jung LS, Wagner P, Nock S

Journal:Anal Biochem

PubMed ID:12531195

'Antibody microarrays have the potential to revolutionize protein expression profiling. The intensity of specific signal produced on a feature of such an array is related to the amount of analyte that is captured from the biological mixture by the immobilized antibody (the "capture agent"). This in turn is a function ... More

N-t-butyl hydroxylamine, a hydrolysis product of alpha-phenyl-N-t-butyl nitrone, is more potent in delaying senescence in human lung fibroblasts.

Authors:Atamna H, Paler-Martínez A, Ames BN

Journal:J Biol Chem

PubMed ID:10702229

'Alpha-phenyl-N-t-butyl nitrone (PBN), a spin trap, scavenges hydroxyl radicals, protects tissues from oxidative injury, and delays senescence of both normal human lung fibroblasts (IMR90) and senescence-accelerated mice. N-t-butyl hydroxylamine and benzaldehyde are the breakdown products of PBN. N-t-Butyl hydroxylamine delays senescence of IMR90 cells at concentrations as low as 10 ... More

Detection of abasic sites and oxidative DNA base damage using an ELISA-like assay.

Authors:Kow YW, Dare A

Journal:Methods

PubMed ID:11020331

'Reactive oxygen species produce a wide spectrum of DNA damage, including oxidative base damage and abasic (AP) sites. Many procedures are available for the quantification and detection of base damage and AP sites. However, either these procedures are laborious or the starting materials are difficult to obtain. A biotinylated aldehyde-specific ... More

Direct visualization of abasic sites on a single DNA molecule using fluorescence microscopy.

Authors:Hirose T, Ohtani T, Muramatsu H, Tanaka A

Journal:Photochem Photobiol

PubMed ID:12194206

'A new method was developed to allow direct visualization of damaged sites on individual DNA molecules. Fluorescence in situ hybridization on extended DNA molecules was modified to detect a single abasic site. Abasic sites were specifically labeled with a biotinylated aldehyde-reactive probe and fluorochrome-conjugated streptavidin. The light emitted by a ... More

A chemoenzymatic approach toward the rapid and sensitive detection of O-GlcNAc posttranslational modifications.

Authors:Khidekel N, Arndt S, Lamarre-Vincent N, Lippert A, Poulin-Kerstien KG, Ramakrishnan B, Qasba PK, Hsieh-Wilson LC

Journal:J Am Chem Soc

PubMed ID:14692737

View all ARP (N-(aminooxyacetyl)-N'-(D-Biotinoyl) hydrazine, Trifluoroacetic Acid Salt) citations