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Milieu BG-11, optimisé pour les cyanobactéries
Gibco™

Milieu BG-11, optimisé pour les cyanobactéries

Le milieu de culture Gibco™ BG-11 Media est optimisé pour la croissance et l’entretien de certaines cyanobactéries, y compris SynechococcusAfficher plus
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RéférenceQuantité
A13799011 L
A13799026 l
Référence A1379901
Prix (EUR)
69,75
Each
Quantité:
1 L
Prix (EUR)
69,75
Each
Le milieu de culture Gibco™ BG-11 Media est optimisé pour la croissance et l’entretien de certaines cyanobactéries, y compris Synechococcus elongates. La formulation est prête à l’emploi à 1 X pour vous permettre d’éviter les étapes laborieuses de préparation des milieux.

Chaque milieu de culture Gibco™ BG-11 est fourni dans le flacon Gibco™ primé conçu pour une utilisation plus facile dans l’armoire de sécurité biologique, réduisant le risque de contamination et vous aidant à effectuer la culture cellulaire de manière plus cohérente. Ensemble, l’emballage et la qualité supérieurs, la plus grande fiabilité et la meilleure cohérence dans la culture des Cyanobactéries, ont pour résultat une meilleure efficacité globale et des données plus robustes (fig. 1, 2).

Usage exclusivement réservé à la recherche. Non utilisé à des fins thérapeutiques ou diagnostiques humaines ou animales.
Spécifications
Type de milieuMilieux d’algues
Méthode de préparationAucun (prêt à l’emploi)
Quantité1 L
Conditions d’expéditionTempérature ambiante
FormeLiquide
Type de produitMilieux de culture
Classe d’organisme cibleCyanobactéries
Unit SizeEach
Contenu et stockage
Les milieux de croissance Gibco™ BG-11 contiennent :

•  Milieu Gibco™ BG-11 1 L — Stocker à 4°C

Foire aux questions (FAQ)

I'm getting low basal expression of my gene of interest when using the pSyn_1 vector. What do you suggest I try?

The promoter for pSyn_1 is the Psc. Psc is a weak constitutive promoter that drives basal expression of your gene of interest. The promoter for pSyn_6, on the other hand, is the psbA promoter (PpsbA). PpsbA is a strong constitutive promoter of the psbA gene (encoding photosystem II protein D1) from Synechococcus elongatus, driving the high level expression of your gene of interest.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the difference between pSyn_1 and pSyn_6?

The promoter for pSyn_1 is the Psc. Psc is a weak constitutive promoter that drives basal expression of your gene of interest. The promoter was taken from the solanesyl diphosphate synthase gene from Synechocystis sp. Strain PCC 6803. Note: Use of this weak constitutive promoter is a good fit for applications that are hindered by strong expression, such as pathway engineering or complementation of mutant genes normally expressed at low levels (Simkovsky et al., 2012).

The promoter for pSyn_6 is the psbA promoter (PpsbA). PpsbA is a strong constitutive promoter of the psbA gene (encoding photosystem II protein D1) from Synechococcus elongatus, driving high-level expression of your gene of interest. The pSyn-6 vector also includes a ribosome-binding site (GAAGGAG) for efficient initiation of translation, as well as a stop codon. A 6xHis TEV and a V5-His epitope tag is also included in the vector backbone for detection/purification of the gene of interest. NS1 (neutral site 1) homologous recombination sites are present in both vectors for the integration of the vector into the Synechococcus elongatus genome.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the shelf-life/competency of Synechococcus?

After 6 months of storage, the Synechococcus did not lose their competency. We have not yet tested longer storage points.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the mechanism of integration when using the Synechococcus expression kits?

A double homologous recombination event occurs between the Neutral Site 1 in the genome and between NSa and NSb in the plasmid. This results in the gene of interest and spectinomycin resistance integrating into the genome within the Neutral Site 1.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the strain of Synechococcus used in your kits?

We no longer offer any strains. Our reagents and protocols were developed using Synechococcus elongatus strain PCC 7942, a model cyanobacterium.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

View all Milieu BG-11, optimisé pour les cyanobactéries FAQs