Vitronectin (VTN-N) Recombinant Human Protein, Truncated
IMPORTANT UPDATE: Vitronectin (VTN-N) Recombinant Human Protein, Truncated, now available under new catalog numbers, A400457 and A400458. Catalog numbers A14700 and A31804 will be discontinued.
Vitronectin (VTN-N) Recombinant Human Protein, Truncated
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Vitronectin (VTN-N) Recombinant Human Protein, Truncated
Vitronectin (VTN-N) Recombinant Human Protein, Truncated
Gibco™

Vitronectin (VTN-N) Recombinant Human Protein, Truncated

Vitronectin (VTN-N) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs).
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RéférenceQuantité
A147001 mL = 0,5 mg
A3180410 mL = 5 mg
Référence A14700
Prix (EUR)
70,00
Each
Ajouter au panier
Quantité:
1 mL = 0,5 mg
Prix (EUR)
70,00
Each
Ajouter au panier

Vitronectin (VTN-N) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). When used with Essential 8 Medium (Cat. No. A1517001), VTN-N has been proven to maintain pluripotency and normal growth characteristics in multiple PSC lines.

  • Proven to maintain pluripotency and normal growth characteristics in multiple PSC lines when used with Essential 8 Medium
  • Supports PSC growth for >50 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages
  • Supports human pluripotent stem cell attachment and survival better than wild-type vitronectin when used in conjunction with Essential 8 Medium
  • Optimized—designed in the laboratory of James Thomson for use with Essential 8 Medium
  • Defined—for reduced variability in your PSC cultures
  • Supports human pluripotent stem cell attachment and survival better than wild type vitronectin
  • Optimal environment for feeder-free pluripotent stem cell culture
  • Defined, recombinant human protein that reduces variability in your PSC cultures compared to human-plasma derived vitronectin and standard basement membrane extracts (BMEs)
For Research Use Only. Not for use in diagnostic procedures.
Spécifications
ClassificationAOF
Concentration500 μg/mL
Niveau d’endotoxinesVery Low
Forme protéiqueRecombinant
Sous-type de protéineVitronectin
Méthode de purificationHPLC
Pureté ou qualité95 %
Quantité1 mL = 0,5 mg
Catégorie de rechercheStem Cell Research
Conditions d’expéditionDry ice
Volume (métrique)1 mL
À utiliser avec (application)Stem Cell Culture
FormeLiquid
RecombinantRecombinant
EspècesHuman
Unit SizeEach
Contenu et stockage
Store at -80°C and protect from light.

Foire aux questions (FAQ)

What substrate should I use with the PSC Definitive Endoderm Induction Kit?

We recommend using Vitronectin (VTN-N) Recombinant Human Protein, Truncated (Cat. No. A14700), but Geltrex LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413301 or Cat. No. A1413302) can also be used.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why are the recommended working concentrations different for CTS Vitronectin (VTN-N) Recombinant Human Protein, Truncated vs. Vitronectin (VTN-N) Recombinant Human Protein, Truncated? Are they not the same protein?

These two proteins are the same but have different protein concentrations due to the different methods used to measure the protein concentrations.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I am interested in using StemFlex Medium in somatic cell reprogramming. What protocol should I follow for blood cells?

We have evaluated the use of Cytotune-iPS 2.0 Sendai Reprogramming Kit (Cat. No. A16517) for somatic cell reprogramming of CD34+ blood cells. For CD34+ cells, follow the instructions provided in the Cytotune 2.0 reprogramming manual for feeder-free reprogramming (pgs. 39-44). On Day 3, you can utilize rhVTN-N (Cat. No. A14700), Geltrex (Cat. No. A1413302), or rhLaminin-521 (Cat. No. A29248 or A29249). From Day 8 onward, rather than feeding daily with Essential 8 Medium, reprogrammed CD34+ cells should be fed every-other-day with StemFlex Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I am interested in using StemFlex Medium in somatic cell reprogramming. What protocol should I follow for human fibroblasts?

We have evaluated the use of Cytotune-iPS 2.0 Sendai Reprogramming Kit (Cat. No. A16517) for somatic cell reprogramming of both neonatal and adult human dermal fibroblasts. For fibroblasts, follow the instructions provided in the Cytotune 2.0 reprogramming manual for feeder-free reprogramming (pgs. 16-20). On Day 7, you may use rhVTN-N (Cat. No. A14700), Geltrex matrix (Cat. No. A1413302), or rhLaminin-521 (Cat. No. A29248 or A29249). From Day 8 onward, rather than feeding daily with Essential 8 Medium, we recommend that you feed reprogrammed fibroblasts every-other-day with StemFlex Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

You recommend Geltrex as the matrix for routine culture of StemFlex Medium. Can I also use Vitronectin or rhLaminin-521?

Yes. We recommend following the coating instructions for Vitronectin or rhLaminin-521 and using the culture and passaging recommendations in the StemFlex protocol.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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