Milieu d’induction neurale de CSP
Milieu d’induction neurale de CSP
Citations et références (18)
Gibco™

Milieu d’induction neurale de CSP

Le milieu d’induction neurale Gibco™ PSC des cellules souches pluripotentes est sans sérum et assure une induction neurale hautement efficaceAfficher plus
Have Questions?
RéférenceQuantité
A1647801500 ml
Référence A1647801
Prix (EUR)
610,00
Each
Ajouter au panier
Quantité:
500 ml
Prix (EUR)
610,00
Each
Ajouter au panier
Le milieu d’induction neurale Gibco™ PSC des cellules souches pluripotentes est sans sérum et assure une induction neurale hautement efficace des cellules souches pluripotentes humaines en à peine 7 jours. Contrairement aux méthodologies existantes, le recours au milieu d’induction neurale des cellules souches pluripotentes n’exige pas d’étape intermédiaire pour la formation de corps embryoïdes, qui suppose plus de temps, de main-d’œuvre et de variabilité. Les cellules souches neurales (NSC) générées à l’aide du milieu d’induction neurale des cellules souches pluripotentes comportent une expression élevée de marqueurs des cellules souches neurales et peuvent être transformées en autres types de cellules neurales.

Le milieu d’induction neurale de CSP permet :

• Induction rapide de cellules souches neurales— induction neurale hautement efficace en à peine 7 jours, sans formation de corps embryoïdes
• Production évolutive de NSC— génère >20 millions de cellules souches neurales à partir d’un million de cellules souches pluripotentes
• Génération de cellules souches neurales de haute qualité— les cellules souches neurales résultantes peuvent être cryopréservées, étendues et différenciées.

Induction rapide des cellules souches neurales
Le milieu d’induction neurale des cellules souches pluripotentes simplifie le flux de travail de génération de CSN en éliminant la nécessité de former des EB ou des rosettes. En éliminant cette étape, le milieu d’induction neurale des cellules souches pluripotentes améliore la reproductibilité avec une induction neurale hautement efficace en à peine 7 jours.

Production évolutive de cellules souches neurales
Le milieu d’induction neurale des cellules souches pluripotentes est robuste et peut générer plus de 20 millions de cellules souches neurales à partir de 1 million de cellules souches pluripotentes. Ce milieu a été testé avec plusieurs lignées hPSC, y compris des SPIh dérivées de l’utilisation des vecteurs de reprogrammation des SPIh épisomiques et du kit de reprogrammation CytoTune™-iPS Sendai.

Génération de cellules souches neurales de haute qualité
Les cellules souches neurales générées par un milieu d’induction neurale de cellules souches pluripotentes ont une expression de >80 % de Sox1, Sox2 et de nestin (marqueurs NSC) et une expression de <1 % d’Oct4 (marqueur de pluripotence). En outre, les cellules souches neurales développées ont maintenu le phénotype, présenté un caryotype normal et ont pu être différenciées dans les neurones, astrocytes et oligodendrocytes.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Type de celluleCellules souches embryonnaires (CSE), cellules souches pluripotentes induites (CSPi), cellules souches humaines
Type de produitMilieu d’induction neurale de CSP
Quantité500 ml
Serum LevelSans sérum
Unit SizeEach
Contenu et stockage
• Milieu de base de 500 ml (stocker entre 2 et 8°C et à l’abri de la lumière)
• Supplément de 10 ml (stocker entre -5 et -20°C et à l’abri de la lumière)

Foire aux questions (FAQ)

Can PSC Neural Induction Medium be used to differentiate hPSCs into neurospheres or neurons?

PSC Neural Induction Medium is a medium to convert hPSCs into NSCs. At day 7 of neural induction, dissociate P0 NSCs with Accutase. The following recommendations can be use for differentiation of neurons or neurospheres: For differentiation of neurons: Use PSC Neural Induction Medium to convert hPSCs into NSCs. At day 7 of neural induction, dissociate P0 NSCs with Accutase. If dissociated NSCs are plated on a Geltrex-coated culture vessel in neural expansion medium, NSCs will grow as a mono-layer. For NSC expansion, it is necessary to treat NSCs with 5 µM ROCK inhibitor Y27632 at the time of plating to prevent cell death if NSCs are under P4. For differentiation of neurospheres: Use PSC Neural Induction Medium to convert hPSCs into NSCs. At day 7 of neural induction, dissociate P0 NSCs with Accutase. If dissociated NSCs are plated into a non-coated flask in neural expansion medium, dissociated NSCs will re-aggregate into small spheres. NSCs in each sphere will proliferate and form big neurospheres. We have not expanded NSCs in neurosphere format in-house. You can test expanding NSCs in neurospheres with and without ROCK inhibitor Y27632 to determine which is optimal for your workflow. Neural stem cells in adherent or neurosphere conditions can be differentiated into neurons using the appropriate protocol.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Have you performed flow cytometry or qPCR studies to determine the expression of NKX2.1 in NSCs generated from iPSC using PSC Neural Induction Medium?

Studies have not been performed using flow cytometry or qPCR to detect NKX2.1 expression in NSCs induced by Neural Induction Medium.

Is it normal to see loss of pluripotency markers (i.e. SSEA-4, Oct-4, and Tra1-60) and still test positive for SOX2 expression when NSCs are cultured in neural expansion medium?

Yes, this is normal. SOX2 is expressed by both hPSCs and hPSC-derived NSCs, so you will see its expression in the induced NSCs from iPSCs.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can NSCs induced by PSC Neural Induction Medium be differentiated into neurons on glass slides/coverslips coated with laminin and poly-L-ornithine?

NSCs induced using the PSC Neural Induction Medium can be differentiated on glass slides/coverslips, but the glass should be cleaned well before coating. We recommend the following cleaning protocol:
1. Treat glass cover slips with1M HCl at RT for 2-3 h on a shaker.
2. Rinse with tap water 5 times, and then double distilled water for 2 times
3. Store cleaned cover slips in 70% ethanol.
4. Dry cover slips by transferring cover slips into culture plate with sterile forceps before poly-L-ornithine and laminin coating.

Can I maintain differentiated neurospheres in Neurobasal+B27+N2+bFGF+EGF?

Neurospheres can be plated on laminin coated culture plates for neuron differentiation. The issue is that it is difficult to control the plating density of neurospheres. Alternatively, neurospheres can be dissociated into single cells and plate single cell suspension at a certain density such as 1-5 x 10^4 cells/cm2 onto laminin coated plates for neuron differentiation. For general neuron differentiation, Neurobasal+B27+N2 can be used. Growth factors such as BDNF and/or GDNF can be added into medium for improving survival of differentiating NSCs.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Milieu d’induction neurale de CSP FAQs

Citations et références (18)

Citations et références
Abstract
A comprehensive protocol for efficient differentiation of human NPCs into electrically competent neurons.
Authors:Romito E,Battistella I,Plakhova V,Paplekaj A,Forastieri C,Toffolo E,Musio C,Conti L,Battaglioli E,Rusconi F
Journal:Journal of neuroscience methods
PubMed ID:39053772
Efficient and rapid derivation of primitive neural stem cells and generation of brain subtype neurons from human pluripotent stem cells.
Authors:Yan Y, Shin S, Jha BS, Liu Q, Sheng J, Li F, Zhan M, Davis J, Bharti K, Zeng X, Rao M, Malik N, Vemuri MC,
Journal:
PubMed ID:24113065
'Human pluripotent stem cells (hPSCs), including human embryonic stem cells and human induced pluripotent stem cells, are unique cell sources for disease modeling, drug discovery screens, and cell therapy applications. The first step in producing neural lineages from hPSCs is the generation of neural stem cells (NSCs). Current methods of ... More
New type of Sendai virus vector provides transgene-free iPS cells derived from chimpanzee blood.
Authors:Fujie Y, Fusaki N, Katayama T, Hamasaki M, Soejima Y, Soga M, Ban H, Hasegawa M, Yamashita S, Kimura S, Suzuki S, Matsuzawa T, Akari H, Era T,
Journal:
PubMed ID:25479600
'Induced pluripotent stem cells (iPSCs) are potentially valuable cell sources for disease models and future therapeutic applications; however, inefficient generation and the presence of integrated transgenes remain as problems limiting their current use. Here, we developed a new Sendai virus vector, TS12KOS, which has improved efficiency, does not integrate into ... More
Differentiation of Human Neural Stem Cells into Motor Neurons Stimulates Mitochondrial Biogenesis and Decreases Glycolytic Flux.
Authors:O'Brien LC, Keeney PM, Bennett JP,
Journal:
PubMed ID:25892363
Differentiation of human pluripotent stem cells (hPSCs) in vitro offers a way to study cell types that are not accessible in living patients. Previous research suggests that hPSCs generate ATP through anaerobic glycolysis, in contrast to mitochondrial oxidative phosphorylation (OXPHOS) in somatic cells; however, specialized cell types have not been ... More
Induced pluripotent stem cell - derived neurons for the study of spinocerebellar ataxia type 3.
Authors:Hansen SK, Stummann TC, Borland H, Hasholt LF, Tümer Z, Nielsen JE, Rasmussen MA, Nielsen TT, Daechsel JC, Fog K, Hyttel P
Journal:Stem Cell Res
PubMed ID:27596958
'The neurodegenerative disease spinocerebellar ataxia type 3 (SCA3) is caused by a CAG-repeat expansion in the ATXN3 gene. In this study, induced pluripotent stem cell (iPSC) lines were established from two SCA3 patients. Dermal fibroblasts were reprogrammed using an integration-free method and the resulting SCA3 iPSCs were differentiated into neurons. ... More
View all Milieu d’induction neurale de CSP citations