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Citations et références (11)
Invitrogen™
7-Amino-4-Methylcoumarin, reference standard
7-amino-4-méthylcoumarine, étalon de référence
| Référence | Quantité |
|---|---|
| A191 | 100 mg |
Référence A191
Prix (EUR)
246,00
Each
Quantité:
100 mg
Prix (EUR)
246,00
Each
Le 7-amino-4-méthylcoumarine (AMC) peut être utilisé comme étalon de référence de fluorescence pour les substrats enzymatiques à base d’AMC, y compris les substrats de caspase à base d’AMC. Les spectres de fluorescence de l’AMC ne sont pas soumis à une variabilité due à la protonation / déprotonation dépendante du pH lorsqu’ils sont dosés près ou au-dessus du pH physiologique.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Émission430 nm
Excitation351 nm
Marqueur ou colorantMéthylcoumarine
Poids moléculaire175.2
Type de produit7-Amino-4-méthylcoumarine
Quantité100 mg
Conditions d’expéditionTempérature ambiante
Type d’étiquetteColorants coumarine
Unit SizeEach
Contenu et stockage
Stocker à température ambiante et à l’abri de la lumière.
Foire aux questions (FAQ)
What solvent should I use to reconstitute 7-Amino-4-Methylcoumarin, reference standard (Cat. No. A191)? Once reconstituted, can the product be aliquoted and stored at -20 degrees C without damaging the molecule?
Citations et références (11)
Citations et références
Abstract
High-performance liquid affinity chromatography and in situ fluorescent labeling on thin-layer chromatography of glycosphingolipids.
Journal:Anal Biochem
PubMed ID:2327579
'A method combining high-performance liquid affinity chromatography and in situ fluorescent labeling on thin-layer chromatography is introduced for determination of glycosphingolipids. Glycolipids in crude extract from rat liver were separated quantitatively from neutral lipids and phospholipids with a phenylboronic acid-derivatized silica gel column. Glycolipids were eluted quantitatively with approximately 98%
Visual detection of peptidase activity using fluorogenic substrates in a microtiter plate assay.
Journal:Anal Biochem
PubMed ID:2339787
'A simple, inexpensive, and sensitive assay for peptidase activity has been devised. The assay was performed in a microtiter plate and was based on fluorogenic peptide substrates, many of which are commercially available. 7-Amino-4-methyl coumarin the fluorescent product liberated during an incubation period of between 1 and 16 h, was
New fluorogenic substrates for subtilisin.
Journal:Chem Pharm Bull (Tokyo)
PubMed ID:3899387
A new fluorescent tag for labeling of saccharides.
Journal:Anal Biochem
PubMed ID:6846798
Potential aldehyde groups of N-acetylglucosamine and its di- and tri-saccharides were coupled with 7-amino-4-methylcoumarin (AMC) by reductive amination in the presence of sodium cyanoborohydride. The products were purified by silica gel chromatography. Acid hydrolysis and digestion with glycosidases gave the expected products. One picomole of the sugar-AMC complexes could be
A new fluorogenic substrate for plasmin.
Journal:Biochem J
PubMed ID:161507
A new fluorogenic peptide substrate for plasmin, 7-(N-succinoylalanylphenylalanyl-lysylamido)-4-methylcoumarin trifluoroacetate salt, was prepared that can be used in a simple and direct assay. The results obtained by the assay method are linear over a wide range of enzyme concentrations and sensitive enough to detect as little as 10(-5) CTA units of