PSC Cardiomyocyte Differentiation Kit
Gibco™

PSC Cardiomyocyte Differentiation Kit

Le kit de différenciation des cardiomyocytes Gibco™ CSP se compose d’un ensemble de milieux de culture exempts de sérum etAfficher plus
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RéférenceQuantité
A29212011 kit
Référence A2921201
Prix (EUR)
652,00
Each
Quantité:
1 kit
Prix (EUR)
652,00
Each
Le kit de différenciation des cardiomyocytes Gibco™ CSP se compose d’un ensemble de milieux de culture exempts de sérum et de xénon qui permettent de différencier efficacement les cellules souches pluripotentes humaines (CSP) des cardiomyocytes en contraction en à peine 8 jours. Contrairement à d’autres méthodes nécessitant plusieurs composants et des tests biologiques d’une durée plus importante, le kit de différenciation des cardiomyocytes CSP génère des cardiomyocytes à partir de cellules souches pluripotentes dans un format de milieu de culture prêt à l’emploi et en moins de temps.

Composé de trois milieux 1X qui ne nécessitent ni décongélation ni mélange, chaque milieu est utilisé consécutivement sur une période totale de 14 jours, donnant des cardiomyocytes fonctionnels qui expriment des marqueurs physiologiques correspondants, se contractent en culture et peuvent ensuite être maintenus en culture pendant > 15 jours (le milieu de maintenance est également vendu séparément (réf. de catalogue A2920801)).

Aucun dégel ou mélange requis
Le kit de différenciation de cardiomyocytes CSP est composé d’un milieu 1X qui ne nécessite pas d’être conservé au congélateur. Aucun dégel ou mélange des réactifs n’est donc utile. Réchauffez simplement le milieu et ajoutez-le à la culture de CSP.

Production rapide de cardiomyocytes
Le kit de différenciation des cardiomyocytes CSP est capable de produire des cardiomyocytes en contraction en seulement 8 jours. Les cardiomyocytes différenciés peuvent ensuite être maintenus en culture pendant > 15 jours.

Production de cardiomyocytes de haute qualité
Les cardiomyocytes produits à l’aide du kit de différenciation des cardiomyocytes CSP sont pertinents sur le plan fonctionnel, exprimant des marqueurs clés tels que TNNT2, Nkx2.5, MYH6 et α-actinine, et se contractant en culture.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Type de celluleCardiomyocytes
Quantité1 kit
Conditions d’expéditionTempérature ambiante
Type de produitKit de différenciation de cardiomyocytes CSP
Unit SizeEach
Contenu et stockage
• Milieu de maintenance des cardiomyocytes, 500 ml
• Milieu A de différenciation des cardiomyocytes, 100 ml
• Milieu B de différenciation des cardiomyocytes, 100 ml

Conserver à une température entre 2 et 8°C, à l’abri de la lumière.

Foire aux questions (FAQ)

What substrate should I use for cardiomyocyte differentiation?

We recommend using Gibco Geltrex Flex LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix (Cat. No. A4000046801, A4000046802, or A4000046803) or Gibco Vitronectin (VTN-N) Recombinant Human Protein, Truncated (Cat. No. A400457 or A400458) for xeno-free applications.

Is StemFlex Medium compatible with downstream differentiation kits available from Thermo Fisher Scientific?

Yes. We have seen compatibility with the following differentiation kits provided by Thermo Fisher Scientific: PSC Cardiomyocyte Differentiation Kit (Cat. No. A2921201), PSC Definitive Endoderm Induction Kit (Cat. No. A3062601), PSC Neural Induction Medium (Cat. No. A1647801), and PSC Dopaminergic Neuron Differentiation Kit (Cat. No. A3147701).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I used the Gibco PSC Cardiomyocyte Differentiation Kit and am not seeing good differentiation with my iPSC line. What should I do?

We recommend always using H9 or H7 ESC line as a control in your experiments. We recommend adjusting the cell density or extending the induction time for difficult-to-differentiate iPSC lines.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I am planning to use the PSC Cardiomyocyte Differentiation Kit. Which markers can I expected my differentiated cells to express?

Cardiomyocytes generated using PSC Cardiomyocyte Differentiation Kit have been tested for key markers such as TNNT2, Nkx2.5, MYH6, and Alpha-Actinin. The Cardiomyocyte Immunocytochemistry Kit (Cat. No. A25973) contains validated antibodies to measure TNNT2 and Nkx2.5 in cultures generated using PSC Cardiomyocyte Differentiation Kit.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I maintain differentiated cells in culture using the Gibco PSC Cardiomyocyte Differentiation Kit?

Differentiated cells can be maintained for a month or longer for long-term studies. We recommend the use of Gibco Geltrex Matrix for long-term cultures.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations et références (10)

Citations et références
Abstract
Cardiac disease modeling using induced pluripotent stem cell-derived human cardiomyocytes.
Authors:Dell'Era P, Benzoni P, Crescini E, Valle M, Xia E, Consiglio A, Memo M,
Journal:
PubMed ID:25815118
Causative mutations and variants associated with cardiac diseases have been found in genes encoding cardiac ion channels, accessory proteins, cytoskeletal components, junctional proteins, and signaling molecules. In most cases the functional evaluation of the genetic alteration has been carried out by expressing the mutated proteins in in-vitro heterologous systems. While ... More
Generation of the human induced pluripotent stem cell (hiPSC) line PSMi004-A from a carrier of the KCNQ1-R594Q mutation.
Authors:Mura M, Lee YK, Pisano F, Ginevrino M, Boni M, Calabrò F, Crotti L, Valente EM, Schwartz PJ, Tse HF, Gnecchi M
Journal:Stem Cell Res
PubMed ID:30974404
'We generated human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts of a male carrier of the heterozygous mutation c.1781?G?>?A p.R594Q on the KCNQ1 gene. hiPSCs, generated using four retroviruses each encoding for OCT4, SOX2, KLF4 and cMYC, display pluripotent stem cell characteristics, and can be differentiated into spontaneously beating ... More
Reprogramming of Urine-Derived Renal Epithelial Cells into iPSCs Using srRNA and Consecutive Differentiation into Beating Cardiomyocytes.
Authors:Steinle H, Weber M, Behring A, Mau-Holzmann U, von Ohle C, Popov AF, Schlensak C, Wendel HP, Avci-Adali M
Journal:Mol Ther Nucleic Acids
PubMed ID:31476669
'The generation of induced pluripotent stem cells (iPSCs) from patient''s somatic cells and the subsequent differentiation into desired cell types opens up numerous possibilities in regenerative medicine and tissue engineering. Adult cardiomyocytes have limited self-renewal capacity; thus, the efficient, safe, and clinically applicable generation of autologous cardiomyocytes is of great ... More
Generation of human iPS cell line CBTCi001-A from dermal fibroblasts obtained from a healthy donor.
Authors:Martins GLS, Paredes BD, Sampaio GLA, Nonaka CKV, da Silva KN, Allahdadi KJ, França LSA, Soares MBP, Dos Santos RR, Souza BSF
Journal:Stem Cell Res
PubMed ID:31706097
Human-induced pluripotent stem cell (hiPSC) CBTCi001-A line was generated from a healthy 30-year old male dermal fibroblasts using non-integrative reprogramming method using episomal-based plasmids expressing OCT4, SOX2, KLF4, and MYCL. Characterization of CBTCi001-A was confirmed by the expression of typical markers of pluripotency and differentiation potential in vitro. ... More
Generation of the human induced pluripotent stem cell (hiPSC) line PSMi007-A from a Long QT Syndrome type 1 patient carrier of two common variants in the NOS1AP gene.
Authors:Mura M, Pisano F, Stefanello M, Ginevrino M, Boni M, Calabrò F, Crotti L, Valente EM, Schwartz PJ, Brink PA, Gnecchi M
Journal:Stem Cell Res
PubMed ID:30878014
We generated human induced pluripotent stem cells (hiPSCs) from a symptomatic Long QT Syndrome (LQTS) type 1 patient, belonging to a South African (SA) founder population segregating the heterozygous mutation c.1022C?>?T p.A341V on the KCNQ1 gene. The patient is also homozygous for the two minor variants rs4657139 and rs16847548 on ... More